Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening

Detalhes bibliográficos
Autor(a) principal: Mata,Gardênia Márcia Silva Campos
Data de Publicação: 2016
Outros Autores: Martins,Evandro, Machado,Solimar Gonçalves, Pinto,Maximiliano Soares, Carvalho,Antônio Fernandes de, Vanetti,Maria Cristina Dantas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749
Resumo: ABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.
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spelling Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripeningListeriaRaw milk cheeseAlternative methodsABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.Sociedade Brasileira de Microbiologia2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749Brazilian Journal of Microbiology v.47 n.3 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.04.006info:eu-repo/semantics/openAccessMata,Gardênia Márcia Silva CamposMartins,EvandroMachado,Solimar GonçalvesPinto,Maximiliano SoaresCarvalho,Antônio Fernandes deVanetti,Maria Cristina Dantaseng2016-08-02T00:00:00Zoai:scielo:S1517-83822016000300749Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-08-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
spellingShingle Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
Mata,Gardênia Márcia Silva Campos
Listeria
Raw milk cheese
Alternative methods
title_short Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_full Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_fullStr Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_full_unstemmed Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_sort Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
author Mata,Gardênia Márcia Silva Campos
author_facet Mata,Gardênia Márcia Silva Campos
Martins,Evandro
Machado,Solimar Gonçalves
Pinto,Maximiliano Soares
Carvalho,Antônio Fernandes de
Vanetti,Maria Cristina Dantas
author_role author
author2 Martins,Evandro
Machado,Solimar Gonçalves
Pinto,Maximiliano Soares
Carvalho,Antônio Fernandes de
Vanetti,Maria Cristina Dantas
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mata,Gardênia Márcia Silva Campos
Martins,Evandro
Machado,Solimar Gonçalves
Pinto,Maximiliano Soares
Carvalho,Antônio Fernandes de
Vanetti,Maria Cristina Dantas
dc.subject.por.fl_str_mv Listeria
Raw milk cheese
Alternative methods
topic Listeria
Raw milk cheese
Alternative methods
description ABSTRACT The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical-chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300749
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2016.04.006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.47 n.3 2016
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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