Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening

Detalhes bibliográficos
Autor(a) principal: Mata, Gardênia Márcia Silva Campos
Data de Publicação: 2016
Outros Autores: Martins, Evandro, Machado, Solimar Gonçalves, Vanetti, Maria Cristina Dantas, Pinto, Maximiliano Soares, Carvalho, Antônio Fernandes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.bjm.2016.04.006
http://www.locus.ufv.br/handle/123456789/12421
Resumo: The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical–chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.
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spelling Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripeningListeriaRaw milk cheeseAlternative methodsThe ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical–chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.Brazilian Journal of Microbiology2017-10-26T11:16:07Z2017-10-26T11:16:07Z2016-04-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15178382https://doi.org/10.1016/j.bjm.2016.04.006http://www.locus.ufv.br/handle/123456789/12421eng(47)3, p.749-756, July–September 2016Mata, Gardênia Márcia Silva CamposMartins, EvandroMachado, Solimar GonçalvesVanetti, Maria Cristina DantasPinto, Maximiliano SoaresCarvalho, Antônio Fernandes deinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:21:16Zoai:locus.ufv.br:123456789/12421Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:21:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
spellingShingle Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
Mata, Gardênia Márcia Silva Campos
Listeria
Raw milk cheese
Alternative methods
title_short Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_full Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_fullStr Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_full_unstemmed Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
title_sort Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
author Mata, Gardênia Márcia Silva Campos
author_facet Mata, Gardênia Márcia Silva Campos
Martins, Evandro
Machado, Solimar Gonçalves
Vanetti, Maria Cristina Dantas
Pinto, Maximiliano Soares
Carvalho, Antônio Fernandes de
author_role author
author2 Martins, Evandro
Machado, Solimar Gonçalves
Vanetti, Maria Cristina Dantas
Pinto, Maximiliano Soares
Carvalho, Antônio Fernandes de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mata, Gardênia Márcia Silva Campos
Martins, Evandro
Machado, Solimar Gonçalves
Vanetti, Maria Cristina Dantas
Pinto, Maximiliano Soares
Carvalho, Antônio Fernandes de
dc.subject.por.fl_str_mv Listeria
Raw milk cheese
Alternative methods
topic Listeria
Raw milk cheese
Alternative methods
description The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical–chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-22
2017-10-26T11:16:07Z
2017-10-26T11:16:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 15178382
https://doi.org/10.1016/j.bjm.2016.04.006
http://www.locus.ufv.br/handle/123456789/12421
identifier_str_mv 15178382
url https://doi.org/10.1016/j.bjm.2016.04.006
http://www.locus.ufv.br/handle/123456789/12421
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv (47)3, p.749-756, July–September 2016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian Journal of Microbiology
publisher.none.fl_str_mv Brazilian Journal of Microbiology
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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