Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | https://doi.org/10.1016/j.bjm.2016.04.006 http://www.locus.ufv.br/handle/123456789/12421 |
Resumo: | The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical–chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation. |
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Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripeningListeriaRaw milk cheeseAlternative methodsThe ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical–chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation.Brazilian Journal of Microbiology2017-10-26T11:16:07Z2017-10-26T11:16:07Z2016-04-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf15178382https://doi.org/10.1016/j.bjm.2016.04.006http://www.locus.ufv.br/handle/123456789/12421eng(47)3, p.749-756, July–September 2016Mata, Gardênia Márcia Silva CamposMartins, EvandroMachado, Solimar GonçalvesVanetti, Maria Cristina DantasPinto, Maximiliano SoaresCarvalho, Antônio Fernandes deinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:21:16Zoai:locus.ufv.br:123456789/12421Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:21:16LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
spellingShingle |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening Mata, Gardênia Márcia Silva Campos Listeria Raw milk cheese Alternative methods |
title_short |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_full |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_fullStr |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_full_unstemmed |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
title_sort |
Performance of two alternative methods for Listeria detection throughout Serro Minas cheese ripening |
author |
Mata, Gardênia Márcia Silva Campos |
author_facet |
Mata, Gardênia Márcia Silva Campos Martins, Evandro Machado, Solimar Gonçalves Vanetti, Maria Cristina Dantas Pinto, Maximiliano Soares Carvalho, Antônio Fernandes de |
author_role |
author |
author2 |
Martins, Evandro Machado, Solimar Gonçalves Vanetti, Maria Cristina Dantas Pinto, Maximiliano Soares Carvalho, Antônio Fernandes de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mata, Gardênia Márcia Silva Campos Martins, Evandro Machado, Solimar Gonçalves Vanetti, Maria Cristina Dantas Pinto, Maximiliano Soares Carvalho, Antônio Fernandes de |
dc.subject.por.fl_str_mv |
Listeria Raw milk cheese Alternative methods |
topic |
Listeria Raw milk cheese Alternative methods |
description |
The ability of pathogens to survive cheese ripening is a food-security concern. Therefore, this study aimed to evaluate the performance of two alternative methods of analysis of Listeria during the ripening of artisanal Minas cheese. These methods were tested and compared with the conventional method: Lateral Flow System™, in cheeses produced on laboratory scale using raw milk collected from different farms and inoculated with Listeria innocua; and VIDAS®-LMO, in cheese samples collected from different manufacturers in Serro, Minas Gerais, Brazil. These samples were also characterized in terms of lactic acid bacteria, coliforms and physical–chemical analysis. In the inoculated samples, L. innocua was detected by Lateral Flow System™ method with 33% false-negative and 68% accuracy results. L. innocua was only detected in the inoculated samples by the conventional method at 60-days of cheese ripening. L. monocytogenes was not detected by the conventional and the VIDAS®-LMO methods in cheese samples collected from different manufacturers, which impairs evaluating the performance of this alternative method. We concluded that the conventional method provided a better recovery of L. innocua throughout cheese ripening, being able to detect L. innocua at 60-day, aging period which is required by the current legislation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-22 2017-10-26T11:16:07Z 2017-10-26T11:16:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
15178382 https://doi.org/10.1016/j.bjm.2016.04.006 http://www.locus.ufv.br/handle/123456789/12421 |
identifier_str_mv |
15178382 |
url |
https://doi.org/10.1016/j.bjm.2016.04.006 http://www.locus.ufv.br/handle/123456789/12421 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
(47)3, p.749-756, July–September 2016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journal of Microbiology |
publisher.none.fl_str_mv |
Brazilian Journal of Microbiology |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
_version_ |
1817559922239340544 |