Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200003 |
Resumo: | A study of the kinetics of denitrification was carried out in the laboratory based on the quantification of N2O, the final product of the activity of denitrifying microorganisms, when the enzymatic reduction of N2O to N2 was blocked by acetylene. Concentrated mixed liquor (sludge from a reactor with intermittent aeration used for sewage treatment) was used as the inoculum, while methanol, acetic acid, glucose, effluent sewage from an anaerobic fluidized bed reactor and synthetic substrate simulating domestic sewage were used as carbon sources. The mean concentration of nitrate was 20 mg/L. Maxima of N2O production and NO3- consumption occurred between 0.5h and 2.0h of incubation using all the carbon sources, which characterized the denitrification process. Acetic acid and methanol were responsible for the highest rates of N2O production. The estimated number of denitrifying microorganisms in the reactor with intermittent aeration, using the MPN technique, varied from 10(9) to 10(10) MPN/g VSS, indicating a high potential for the occurrence of denitrification. |
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Brazilian Journal of Microbiology |
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Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbondenitrifying activitydenitrifying bacteriadenitrificationtertiary treatment of domestic sewageA study of the kinetics of denitrification was carried out in the laboratory based on the quantification of N2O, the final product of the activity of denitrifying microorganisms, when the enzymatic reduction of N2O to N2 was blocked by acetylene. Concentrated mixed liquor (sludge from a reactor with intermittent aeration used for sewage treatment) was used as the inoculum, while methanol, acetic acid, glucose, effluent sewage from an anaerobic fluidized bed reactor and synthetic substrate simulating domestic sewage were used as carbon sources. The mean concentration of nitrate was 20 mg/L. Maxima of N2O production and NO3- consumption occurred between 0.5h and 2.0h of incubation using all the carbon sources, which characterized the denitrification process. Acetic acid and methanol were responsible for the highest rates of N2O production. The estimated number of denitrifying microorganisms in the reactor with intermittent aeration, using the MPN technique, varied from 10(9) to 10(10) MPN/g VSS, indicating a high potential for the occurrence of denitrification.Sociedade Brasileira de Microbiologia2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200003Brazilian Journal of Microbiology v.34 n.2 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000200003info:eu-repo/semantics/openAccessMarchetto,MargaridaGianotti,Eloisa PozziCampos,José RobertoPires,Roberto CletoMoraes,Elizabeth de Mattoseng2004-01-12T00:00:00Zoai:scielo:S1517-83822003000200003Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-01-12T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
title |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
spellingShingle |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon Marchetto,Margarida denitrifying activity denitrifying bacteria denitrification tertiary treatment of domestic sewage |
title_short |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
title_full |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
title_fullStr |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
title_full_unstemmed |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
title_sort |
Estimate of denitrifying microbiota in tertiary sewage treatment and kinetics of the denitrification process using different sources of carbon |
author |
Marchetto,Margarida |
author_facet |
Marchetto,Margarida Gianotti,Eloisa Pozzi Campos,José Roberto Pires,Roberto Cleto Moraes,Elizabeth de Mattos |
author_role |
author |
author2 |
Gianotti,Eloisa Pozzi Campos,José Roberto Pires,Roberto Cleto Moraes,Elizabeth de Mattos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Marchetto,Margarida Gianotti,Eloisa Pozzi Campos,José Roberto Pires,Roberto Cleto Moraes,Elizabeth de Mattos |
dc.subject.por.fl_str_mv |
denitrifying activity denitrifying bacteria denitrification tertiary treatment of domestic sewage |
topic |
denitrifying activity denitrifying bacteria denitrification tertiary treatment of domestic sewage |
description |
A study of the kinetics of denitrification was carried out in the laboratory based on the quantification of N2O, the final product of the activity of denitrifying microorganisms, when the enzymatic reduction of N2O to N2 was blocked by acetylene. Concentrated mixed liquor (sludge from a reactor with intermittent aeration used for sewage treatment) was used as the inoculum, while methanol, acetic acid, glucose, effluent sewage from an anaerobic fluidized bed reactor and synthetic substrate simulating domestic sewage were used as carbon sources. The mean concentration of nitrate was 20 mg/L. Maxima of N2O production and NO3- consumption occurred between 0.5h and 2.0h of incubation using all the carbon sources, which characterized the denitrification process. Acetic acid and methanol were responsible for the highest rates of N2O production. The estimated number of denitrifying microorganisms in the reactor with intermittent aeration, using the MPN technique, varied from 10(9) to 10(10) MPN/g VSS, indicating a high potential for the occurrence of denitrification. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822003000200003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.34 n.2 2003 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199615995904 |