Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033 |
Resumo: | Trichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole, 5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found. Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The dendogram analysis indicated that almost all strains showed similarity >0.9 among them and all strains were multidrug-resistant. This study brings new results on the identification and genotyping of T. asahii isolated from Brazilian ICU patients and information about their antifungal drugs susceptibility. |
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Brazilian Journal of Microbiology |
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Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patientsTrichosporonPCRRAPDsusceptibilityantifungal drugsTrichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole, 5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found. Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The dendogram analysis indicated that almost all strains showed similarity >0.9 among them and all strains were multidrug-resistant. This study brings new results on the identification and genotyping of T. asahii isolated from Brazilian ICU patients and information about their antifungal drugs susceptibility.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300033info:eu-repo/semantics/openAccessSilva,Rosana Bellan de OliveiraFusco-Almeida,Ana MarisaMatsumoto,Marcelo TeruyukiBaeza,Lilian CristianeBenaducci,TatianeMendes-Giannini,Maria José Soareseng2008-10-02T00:00:00Zoai:scielo:S1517-83822008000300033Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
title |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
spellingShingle |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients Silva,Rosana Bellan de Oliveira Trichosporon PCR RAPD susceptibility antifungal drugs |
title_short |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
title_full |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
title_fullStr |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
title_full_unstemmed |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
title_sort |
Genetic diversity and antifungal susceptibility testing of Trichosporon asahii isolated of Intensive Care Units patients |
author |
Silva,Rosana Bellan de Oliveira |
author_facet |
Silva,Rosana Bellan de Oliveira Fusco-Almeida,Ana Marisa Matsumoto,Marcelo Teruyuki Baeza,Lilian Cristiane Benaducci,Tatiane Mendes-Giannini,Maria José Soares |
author_role |
author |
author2 |
Fusco-Almeida,Ana Marisa Matsumoto,Marcelo Teruyuki Baeza,Lilian Cristiane Benaducci,Tatiane Mendes-Giannini,Maria José Soares |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Rosana Bellan de Oliveira Fusco-Almeida,Ana Marisa Matsumoto,Marcelo Teruyuki Baeza,Lilian Cristiane Benaducci,Tatiane Mendes-Giannini,Maria José Soares |
dc.subject.por.fl_str_mv |
Trichosporon PCR RAPD susceptibility antifungal drugs |
topic |
Trichosporon PCR RAPD susceptibility antifungal drugs |
description |
Trichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole, 5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found. Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The dendogram analysis indicated that almost all strains showed similarity >0.9 among them and all strains were multidrug-resistant. This study brings new results on the identification and genotyping of T. asahii isolated from Brazilian ICU patients and information about their antifungal drugs susceptibility. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000300033 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.3 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201881968640 |