In vitro methods for antifungal susceptibility testing of Trichophyton spp.
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Publication Date: | 2006 |
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Language: | eng |
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Download full: | http://www.repositorio.ufop.br/handle/123456789/4015 https://doi.org/10.1016/j.mycres.2006.08.006 |
Summary: | In general, methods to test the susceptibility of fungi to antifungal drugs require standardized techniques, but so far there is no methodology that is widely applicable to dermatophytes. Here we introduced modifications to the protocols from documents of the National Committee for Clinical Laboratory Standards (CLSI) M38-A and the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) that are usually applied to moulds and fermentative yeasts, in order to adjust the conditions for the growth of dermatophytes. The modifications included: growth on potato dextrose agar supplemented with 2 % in-house rice flour to encourage sporulation, the addition of 2 % glucose to the culture media (RPMI-1640), and an incubation temperature of 28 _C. In addition, the incubation period was 7 d, the minimum inhibitory concentration (MIC) was defined as 80 % growth inhibition endpoints for azole agents, and the inocula only contained microconidia. Results obtained by both tested methodologies were very similar to the ones reported by other researchers. MIC90 (MIC at which 90% of isolates tested were inhibited) values were identical for four out of five antifungal drugs tested and there was only a difference of one or two dilutions when MIC50 values were compared. Although the modifications introduced did not interfere with the results, more studies are necessary to establish a standard technique to test susceptibility of dermatophytes to antifungal drugs. |
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Barros, Maria Elisabete da SilvaSantos, Daniel de AssisHamdan, Júnia Soares2014-11-20T16:55:38Z2014-11-20T16:55:38Z2006BARROS, M. E. da S.; SANTOS, D. de A.; HAMDAN, J. S. In vitro methods for antifungal susceptibility testing of Trichophyton spp. Mycological Research, v. 110, n. 11, p. 1355-1360, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0953756206002589>. Acesso em: 20 ago. 2014.0953-7562http://www.repositorio.ufop.br/handle/123456789/4015https://doi.org/10.1016/j.mycres.2006.08.006In general, methods to test the susceptibility of fungi to antifungal drugs require standardized techniques, but so far there is no methodology that is widely applicable to dermatophytes. Here we introduced modifications to the protocols from documents of the National Committee for Clinical Laboratory Standards (CLSI) M38-A and the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) that are usually applied to moulds and fermentative yeasts, in order to adjust the conditions for the growth of dermatophytes. The modifications included: growth on potato dextrose agar supplemented with 2 % in-house rice flour to encourage sporulation, the addition of 2 % glucose to the culture media (RPMI-1640), and an incubation temperature of 28 _C. In addition, the incubation period was 7 d, the minimum inhibitory concentration (MIC) was defined as 80 % growth inhibition endpoints for azole agents, and the inocula only contained microconidia. Results obtained by both tested methodologies were very similar to the ones reported by other researchers. MIC90 (MIC at which 90% of isolates tested were inhibited) values were identical for four out of five antifungal drugs tested and there was only a difference of one or two dilutions when MIC50 values were compared. Although the modifications introduced did not interfere with the results, more studies are necessary to establish a standard technique to test susceptibility of dermatophytes to antifungal drugs.Antifungal drugsDermatophytesMicrodilutionSusceptibility testingIn vitro methods for antifungal susceptibility testing of Trichophyton spp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Mycological Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/4015/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_VitroMethodsAntifungal.pdfARTIGO_VitroMethodsAntifungal.pdfapplication/pdf134938http://www.repositorio.ufop.br/bitstream/123456789/4015/1/ARTIGO_VitroMethodsAntifungal.pdf82b081381fe9bf7d835e4011bcc08d84MD51123456789/40152019-05-13 17:35:54.443oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-05-13T21:35:54Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
title |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
spellingShingle |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. Barros, Maria Elisabete da Silva Antifungal drugs Dermatophytes Microdilution Susceptibility testing |
title_short |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
title_full |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
title_fullStr |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
title_full_unstemmed |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
title_sort |
In vitro methods for antifungal susceptibility testing of Trichophyton spp. |
author |
Barros, Maria Elisabete da Silva |
author_facet |
Barros, Maria Elisabete da Silva Santos, Daniel de Assis Hamdan, Júnia Soares |
author_role |
author |
author2 |
Santos, Daniel de Assis Hamdan, Júnia Soares |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Barros, Maria Elisabete da Silva Santos, Daniel de Assis Hamdan, Júnia Soares |
dc.subject.por.fl_str_mv |
Antifungal drugs Dermatophytes Microdilution Susceptibility testing |
topic |
Antifungal drugs Dermatophytes Microdilution Susceptibility testing |
description |
In general, methods to test the susceptibility of fungi to antifungal drugs require standardized techniques, but so far there is no methodology that is widely applicable to dermatophytes. Here we introduced modifications to the protocols from documents of the National Committee for Clinical Laboratory Standards (CLSI) M38-A and the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) that are usually applied to moulds and fermentative yeasts, in order to adjust the conditions for the growth of dermatophytes. The modifications included: growth on potato dextrose agar supplemented with 2 % in-house rice flour to encourage sporulation, the addition of 2 % glucose to the culture media (RPMI-1640), and an incubation temperature of 28 _C. In addition, the incubation period was 7 d, the minimum inhibitory concentration (MIC) was defined as 80 % growth inhibition endpoints for azole agents, and the inocula only contained microconidia. Results obtained by both tested methodologies were very similar to the ones reported by other researchers. MIC90 (MIC at which 90% of isolates tested were inhibited) values were identical for four out of five antifungal drugs tested and there was only a difference of one or two dilutions when MIC50 values were compared. Although the modifications introduced did not interfere with the results, more studies are necessary to establish a standard technique to test susceptibility of dermatophytes to antifungal drugs. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2014-11-20T16:55:38Z |
dc.date.available.fl_str_mv |
2014-11-20T16:55:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARROS, M. E. da S.; SANTOS, D. de A.; HAMDAN, J. S. In vitro methods for antifungal susceptibility testing of Trichophyton spp. Mycological Research, v. 110, n. 11, p. 1355-1360, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0953756206002589>. Acesso em: 20 ago. 2014. |
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http://www.repositorio.ufop.br/handle/123456789/4015 |
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0953-7562 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.mycres.2006.08.006 |
identifier_str_mv |
BARROS, M. E. da S.; SANTOS, D. de A.; HAMDAN, J. S. In vitro methods for antifungal susceptibility testing of Trichophyton spp. Mycological Research, v. 110, n. 11, p. 1355-1360, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0953756206002589>. Acesso em: 20 ago. 2014. 0953-7562 |
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http://www.repositorio.ufop.br/handle/123456789/4015 https://doi.org/10.1016/j.mycres.2006.08.006 |
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