Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system

Detalhes bibliográficos
Autor(a) principal: Santos,Juliana A.
Data de Publicação: 2007
Outros Autores: Amaral,Marcel C.O., Araújo,Thiago A.S., Fernandes,Késsia G.C., Chaves,Adilson C., Morais,Márcia M.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100013
Resumo: A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.
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spelling Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous systemextracellular lipaseTrichosporonaqueous two phase systemPEGA lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.Sociedade Brasileira de Microbiologia2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100013Brazilian Journal of Microbiology v.38 n.1 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000100013info:eu-repo/semantics/openAccessSantos,Juliana A.Amaral,Marcel C.O.Araújo,Thiago A.S.Fernandes,Késsia G.C.Chaves,Adilson C.Morais,Márcia M.C.eng2007-04-17T00:00:00Zoai:scielo:S1517-83822007000100013Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-04-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
title Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
spellingShingle Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
Santos,Juliana A.
extracellular lipase
Trichosporon
aqueous two phase system
PEG
title_short Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
title_full Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
title_fullStr Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
title_full_unstemmed Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
title_sort Isolation of a lipase-producing Trichosporon spp and enzyme extraction by two-phase aqueous system
author Santos,Juliana A.
author_facet Santos,Juliana A.
Amaral,Marcel C.O.
Araújo,Thiago A.S.
Fernandes,Késsia G.C.
Chaves,Adilson C.
Morais,Márcia M.C.
author_role author
author2 Amaral,Marcel C.O.
Araújo,Thiago A.S.
Fernandes,Késsia G.C.
Chaves,Adilson C.
Morais,Márcia M.C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos,Juliana A.
Amaral,Marcel C.O.
Araújo,Thiago A.S.
Fernandes,Késsia G.C.
Chaves,Adilson C.
Morais,Márcia M.C.
dc.subject.por.fl_str_mv extracellular lipase
Trichosporon
aqueous two phase system
PEG
topic extracellular lipase
Trichosporon
aqueous two phase system
PEG
description A lipase-producing yeast strain isolated from crude cheese and identified as Trichosporon spp produced 7.3 U/mL (59.3 U/µg) after 72h of cultivation. Lipase showed optimum activity at pH 7.0-8.0 and 45-50ºC. Extraction by the two-phase aqueous system (PEG-phosphate salts) showed an elevated recuperation (99.8%) of enzymatic activity in the PEG phase.
publishDate 2007
dc.date.none.fl_str_mv 2007-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000100013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.1 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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