Template preparation for rapid PCR in Colletotrichum lindemuthianum

Detalhes bibliográficos
Autor(a) principal: Roca,M. Gabriela
Data de Publicação: 2003
Outros Autores: Davide,Lisete C., Wheals,Alan E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
Resumo: Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
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spelling Template preparation for rapid PCR in Colletotrichum lindemuthianumanthracnosehygromycin resistanceinternal transcribed spacerrapid screeningPCR methodologyIsolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).Sociedade Brasileira de Microbiologia2003-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003Brazilian Journal of Microbiology v.34 n.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000100003info:eu-repo/semantics/openAccessRoca,M. GabrielaDavide,Lisete C.Wheals,Alan E.eng2003-06-30T00:00:00Zoai:scielo:S1517-83822003000100003Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2003-06-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Template preparation for rapid PCR in Colletotrichum lindemuthianum
title Template preparation for rapid PCR in Colletotrichum lindemuthianum
spellingShingle Template preparation for rapid PCR in Colletotrichum lindemuthianum
Roca,M. Gabriela
anthracnose
hygromycin resistance
internal transcribed spacer
rapid screening
PCR methodology
title_short Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_full Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_fullStr Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_full_unstemmed Template preparation for rapid PCR in Colletotrichum lindemuthianum
title_sort Template preparation for rapid PCR in Colletotrichum lindemuthianum
author Roca,M. Gabriela
author_facet Roca,M. Gabriela
Davide,Lisete C.
Wheals,Alan E.
author_role author
author2 Davide,Lisete C.
Wheals,Alan E.
author2_role author
author
dc.contributor.author.fl_str_mv Roca,M. Gabriela
Davide,Lisete C.
Wheals,Alan E.
dc.subject.por.fl_str_mv anthracnose
hygromycin resistance
internal transcribed spacer
rapid screening
PCR methodology
topic anthracnose
hygromycin resistance
internal transcribed spacer
rapid screening
PCR methodology
description Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp).
publishDate 2003
dc.date.none.fl_str_mv 2003-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 n.1 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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