Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300006 |
Resumo: | Microbiological and chemical changes were evaluated in freshwater prawn stored under refrigeration at 0°C and 5°C during 10 days, with special emphasis on indole production as a chemical spoilage indicator. The total psychrotrophic and indole positive microflora were mainly mesophilic, with indole positive microorganisms being less than 10% of the total microflora after 10 days storage under refrigeration. Bacteria from Enterobacteriaceae and Vibrionaceae families prevailed among the isolated indole positive strains. The use of the Most Probable Number-MPN method, using tryptone broth as culture medium, was the most reliable approach for the quantitative evaluation of the indole positive microflora. The stored samples showed increases in pH, L-tryptophan and total volatile bases (TVB), which were more intensive at 5°C. The psychrotrophic counts and TVB values of samples stored at 0°C were lower than the recommended limits (107 CFU/g and 30 mg N/100g, respectively), even after 10 days storage. However, in samples stored at 5°C, these values were reached after 10 and 5 days, respectively. The presence of indole in levels above the limit recommended by FDA/USA (25 mug/100g) was confirmed in only one sample, suggesting that this substance, alone, wouldnt be a good indicator of freshwater prawn quality stored under refrigeration. |
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Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigerationfreshwater prawnshelf lifeindoleMicrobiological and chemical changes were evaluated in freshwater prawn stored under refrigeration at 0°C and 5°C during 10 days, with special emphasis on indole production as a chemical spoilage indicator. The total psychrotrophic and indole positive microflora were mainly mesophilic, with indole positive microorganisms being less than 10% of the total microflora after 10 days storage under refrigeration. Bacteria from Enterobacteriaceae and Vibrionaceae families prevailed among the isolated indole positive strains. The use of the Most Probable Number-MPN method, using tryptone broth as culture medium, was the most reliable approach for the quantitative evaluation of the indole positive microflora. The stored samples showed increases in pH, L-tryptophan and total volatile bases (TVB), which were more intensive at 5°C. The psychrotrophic counts and TVB values of samples stored at 0°C were lower than the recommended limits (107 CFU/g and 30 mg N/100g, respectively), even after 10 days storage. However, in samples stored at 5°C, these values were reached after 10 and 5 days, respectively. The presence of indole in levels above the limit recommended by FDA/USA (25 mug/100g) was confirmed in only one sample, suggesting that this substance, alone, wouldnt be a good indicator of freshwater prawn quality stored under refrigeration.Sociedade Brasileira de Microbiologia2000-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300006Brazilian Journal of Microbiology v.31 n.3 2000reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822000000300006info:eu-repo/semantics/openAccessLeitão,Mauro Faber de FreitasRios,Daniel de Pinho Astacioeng2001-03-16T00:00:00Zoai:scielo:S1517-83822000000300006Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2001-03-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
title |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
spellingShingle |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration Leitão,Mauro Faber de Freitas freshwater prawn shelf life indole |
title_short |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
title_full |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
title_fullStr |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
title_full_unstemmed |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
title_sort |
Microbiological and chemical changes in freshwater prawn (Macrobrachium rosembergii) stored under refrigeration |
author |
Leitão,Mauro Faber de Freitas |
author_facet |
Leitão,Mauro Faber de Freitas Rios,Daniel de Pinho Astacio |
author_role |
author |
author2 |
Rios,Daniel de Pinho Astacio |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Leitão,Mauro Faber de Freitas Rios,Daniel de Pinho Astacio |
dc.subject.por.fl_str_mv |
freshwater prawn shelf life indole |
topic |
freshwater prawn shelf life indole |
description |
Microbiological and chemical changes were evaluated in freshwater prawn stored under refrigeration at 0°C and 5°C during 10 days, with special emphasis on indole production as a chemical spoilage indicator. The total psychrotrophic and indole positive microflora were mainly mesophilic, with indole positive microorganisms being less than 10% of the total microflora after 10 days storage under refrigeration. Bacteria from Enterobacteriaceae and Vibrionaceae families prevailed among the isolated indole positive strains. The use of the Most Probable Number-MPN method, using tryptone broth as culture medium, was the most reliable approach for the quantitative evaluation of the indole positive microflora. The stored samples showed increases in pH, L-tryptophan and total volatile bases (TVB), which were more intensive at 5°C. The psychrotrophic counts and TVB values of samples stored at 0°C were lower than the recommended limits (107 CFU/g and 30 mg N/100g, respectively), even after 10 days storage. However, in samples stored at 5°C, these values were reached after 10 and 5 days, respectively. The presence of indole in levels above the limit recommended by FDA/USA (25 mug/100g) was confirmed in only one sample, suggesting that this substance, alone, wouldnt be a good indicator of freshwater prawn quality stored under refrigeration. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822000000300006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.31 n.3 2000 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122198807543808 |