Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk

Detalhes bibliográficos
Autor(a) principal: Beloti,Vanerli
Data de Publicação: 2002
Outros Autores: Barros,Márcia de Aguiar Ferreira, Nunes,Maurício Pinto, Santana,Elsa Helena Walter de, Nero,Luís Augusto, Souza,Juliana Aparecida de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010
Resumo: Readycult™ - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult™ - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult™ - LMX. The Readycult™ - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for total coliforms (r: 0.8224) and for E. coli (r: 0.8603). The two methods yielded similar results, but Readycult™ - LMX was easier to use. In addition, results were available as early as 24h.
id SBM-1_cdf89fcffa37aadfb1a2d5569e277030
oai_identifier_str oai:scielo:S1517-83822002000100010
network_acronym_str SBM-1
network_name_str Brazilian Journal of Microbiology
repository_id_str
spelling Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milkReadycult™ - LMXX-GALMUGmilkEscherichia colitotal coliformsReadycult™ - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult™ - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult™ - LMX. The Readycult™ - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for total coliforms (r: 0.8224) and for E. coli (r: 0.8603). The two methods yielded similar results, but Readycult™ - LMX was easier to use. In addition, results were available as early as 24h.Sociedade Brasileira de Microbiologia2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010Brazilian Journal of Microbiology v.33 n.1 2002reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822002000100010info:eu-repo/semantics/openAccessBeloti,VanerliBarros,Márcia de Aguiar FerreiraNunes,Maurício PintoSantana,Elsa Helena Walter deNero,Luís AugustoSouza,Juliana Aparecida deeng2002-09-16T00:00:00Zoai:scielo:S1517-83822002000100010Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-09-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
title Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
spellingShingle Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
Beloti,Vanerli
Readycult™ - LMX
X-GAL
MUG
milk
Escherichia coli
total coliforms
title_short Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
title_full Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
title_fullStr Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
title_full_unstemmed Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
title_sort Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk
author Beloti,Vanerli
author_facet Beloti,Vanerli
Barros,Márcia de Aguiar Ferreira
Nunes,Maurício Pinto
Santana,Elsa Helena Walter de
Nero,Luís Augusto
Souza,Juliana Aparecida de
author_role author
author2 Barros,Márcia de Aguiar Ferreira
Nunes,Maurício Pinto
Santana,Elsa Helena Walter de
Nero,Luís Augusto
Souza,Juliana Aparecida de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Beloti,Vanerli
Barros,Márcia de Aguiar Ferreira
Nunes,Maurício Pinto
Santana,Elsa Helena Walter de
Nero,Luís Augusto
Souza,Juliana Aparecida de
dc.subject.por.fl_str_mv Readycult™ - LMX
X-GAL
MUG
milk
Escherichia coli
total coliforms
topic Readycult™ - LMX
X-GAL
MUG
milk
Escherichia coli
total coliforms
description Readycult™ - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult™ - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult™ - LMX. The Readycult™ - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for total coliforms (r: 0.8224) and for E. coli (r: 0.8603). The two methods yielded similar results, but Readycult™ - LMX was easier to use. In addition, results were available as early as 24h.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822002000100010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.33 n.1 2002
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122199244800000

Registros relacionados