Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005 |
Resumo: | The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs and the mean values were 87.2 AH50/ml. No significant differences were observed in relation to sex and age. The alternative pathway activation by P. brasiliensis was higher in serum samples from adult dogs when compared to puppies and aged dogs (p < 0.05). This is the first report of dog alternative complement pathway activation by P. brasiliensis and suggests that it may play a protective role in canine paracoccidioidomycosis. |
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Brazilian Journal of Microbiology |
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Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensisParacoccidioidomycosisdogcomplementThe dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs and the mean values were 87.2 AH50/ml. No significant differences were observed in relation to sex and age. The alternative pathway activation by P. brasiliensis was higher in serum samples from adult dogs when compared to puppies and aged dogs (p < 0.05). This is the first report of dog alternative complement pathway activation by P. brasiliensis and suggests that it may play a protective role in canine paracoccidioidomycosis.Sociedade Brasileira de Microbiologia2009-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005Brazilian Journal of Microbiology v.40 n.2 2009reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822009000200005info:eu-repo/semantics/openAccessBianchini,A.A.C.Petroni,T.F.Fedatto,P.F.Bianchini,R.R.Venancio,E.J.Itano,E.N.Ono,M.A.eng2009-07-24T00:00:00Zoai:scielo:S1517-83822009000200005Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-07-24T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
title |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
spellingShingle |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis Bianchini,A.A.C. Paracoccidioidomycosis dog complement |
title_short |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
title_full |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
title_fullStr |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
title_full_unstemmed |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
title_sort |
Activation of the alternative complement pathway in canine normal serum by Paracoccidioides brasiliensis |
author |
Bianchini,A.A.C. |
author_facet |
Bianchini,A.A.C. Petroni,T.F. Fedatto,P.F. Bianchini,R.R. Venancio,E.J. Itano,E.N. Ono,M.A. |
author_role |
author |
author2 |
Petroni,T.F. Fedatto,P.F. Bianchini,R.R. Venancio,E.J. Itano,E.N. Ono,M.A. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bianchini,A.A.C. Petroni,T.F. Fedatto,P.F. Bianchini,R.R. Venancio,E.J. Itano,E.N. Ono,M.A. |
dc.subject.por.fl_str_mv |
Paracoccidioidomycosis dog complement |
topic |
Paracoccidioidomycosis dog complement |
description |
The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs and the mean values were 87.2 AH50/ml. No significant differences were observed in relation to sex and age. The alternative pathway activation by P. brasiliensis was higher in serum samples from adult dogs when compared to puppies and aged dogs (p < 0.05). This is the first report of dog alternative complement pathway activation by P. brasiliensis and suggests that it may play a protective role in canine paracoccidioidomycosis. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822009000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.40 n.2 2009 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122202258407424 |