Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice

Detalhes bibliográficos
Autor(a) principal: Vasconcelos,Ana Luiza Sampaio
Data de Publicação: 2003
Outros Autores: Nicoli,Jacques Robert, Nardi,Regina Maria Drummond
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007
Resumo: In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following proportionally the L. murinus population levels in the respective intestinal portions. Concluding, the present study showed that L. murinus association in gnotobiotic mice delayed the death after an oral challenge with S. Typhimurium and that inhibitory diffusible compounds obtained in in vitro assays were also produced in vivo and may be responsible for this effect.
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spelling Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic miceantagonismSalmonellaLactobacillusgnotobiotic miceIn the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following proportionally the L. murinus population levels in the respective intestinal portions. Concluding, the present study showed that L. murinus association in gnotobiotic mice delayed the death after an oral challenge with S. Typhimurium and that inhibitory diffusible compounds obtained in in vitro assays were also produced in vivo and may be responsible for this effect.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500007info:eu-repo/semantics/openAccessVasconcelos,Ana Luiza SampaioNicoli,Jacques RobertNardi,Regina Maria Drummondeng2004-11-30T00:00:00Zoai:scielo:S1517-83822003000500007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
title Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
spellingShingle Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
Vasconcelos,Ana Luiza Sampaio
antagonism
Salmonella
Lactobacillus
gnotobiotic mice
title_short Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
title_full Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
title_fullStr Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
title_full_unstemmed Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
title_sort Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice
author Vasconcelos,Ana Luiza Sampaio
author_facet Vasconcelos,Ana Luiza Sampaio
Nicoli,Jacques Robert
Nardi,Regina Maria Drummond
author_role author
author2 Nicoli,Jacques Robert
Nardi,Regina Maria Drummond
author2_role author
author
dc.contributor.author.fl_str_mv Vasconcelos,Ana Luiza Sampaio
Nicoli,Jacques Robert
Nardi,Regina Maria Drummond
dc.subject.por.fl_str_mv antagonism
Salmonella
Lactobacillus
gnotobiotic mice
topic antagonism
Salmonella
Lactobacillus
gnotobiotic mice
description In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following proportionally the L. murinus population levels in the respective intestinal portions. Concluding, the present study showed that L. murinus association in gnotobiotic mice delayed the death after an oral challenge with S. Typhimurium and that inhibitory diffusible compounds obtained in in vitro assays were also produced in vivo and may be responsible for this effect.
publishDate 2003
dc.date.none.fl_str_mv 2003-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000500007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 suppl.1 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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