Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/100119 |
Resumo: | Salmonella enterica serovar Enteritidis is one of the main pathogens responsible for foodborne illness in Brazil. Probiotic bacteria can play a role in defense and recovery from enteropathogenic infections. In this study, the ability of Lactobacillus acidophilus LA10 to colonise and exert antagonistic effects in the gastrointestinal tract was tested before and during experimental infection in conventional mice contaminated with S. Enteritidis (SE86). A dose of 0.1 mL containing 108 viable cells of SE86 and L. acidophilus LA10 was orally administered by gavage to mice. The experiment was divided into groups. As a negative control, Group 1 was administered only sterile saline solution. As a positive control, Group 2 was administered only SE86. Group 3 was first administered SE86, and after 10 days, treated with L. acidophilus LA10. Group 4 was first administered L. acidophilus LA10, and after 10 days, challenged with SE86. The results demonstrated that a significant number of SE86 cells were able to colonize the gastrointestinal tract of mice, specifically in the colon and ileum. L. acidophilus LA10 demonstrated an antagonistic effect against SE86, with better results observed for Group 3 over Group 4. Thus, L. acidophilus LA10 shows potential antagonistic effects against S. Enteritidis SE86, especially if administered after infection. |
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Scapin, DianeGrando, Williani FabíolaRossi, Eliandra MirleiPerez, Karla JoseaneMalheiros, Patricia da SilvaTondo, Eduardo Cesar2014-08-12T02:10:40Z20131517-8382http://hdl.handle.net/10183/100119000921675Salmonella enterica serovar Enteritidis is one of the main pathogens responsible for foodborne illness in Brazil. Probiotic bacteria can play a role in defense and recovery from enteropathogenic infections. In this study, the ability of Lactobacillus acidophilus LA10 to colonise and exert antagonistic effects in the gastrointestinal tract was tested before and during experimental infection in conventional mice contaminated with S. Enteritidis (SE86). A dose of 0.1 mL containing 108 viable cells of SE86 and L. acidophilus LA10 was orally administered by gavage to mice. The experiment was divided into groups. As a negative control, Group 1 was administered only sterile saline solution. As a positive control, Group 2 was administered only SE86. Group 3 was first administered SE86, and after 10 days, treated with L. acidophilus LA10. Group 4 was first administered L. acidophilus LA10, and after 10 days, challenged with SE86. The results demonstrated that a significant number of SE86 cells were able to colonize the gastrointestinal tract of mice, specifically in the colon and ileum. L. acidophilus LA10 demonstrated an antagonistic effect against SE86, with better results observed for Group 3 over Group 4. Thus, L. acidophilus LA10 shows potential antagonistic effects against S. Enteritidis SE86, especially if administered after infection.application/pdfengBrazilian journal of microbiology. São Paulo. Vol. 44, no. 1 (Jan./Mar. 2013), p. 57-61Salmonella enteritidisProbióticoLactobacillus acidophilusS. Enteritidis SE86ProbioticsMiceLactobacillus acidophilus LA10Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in miceinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000921675.pdf000921675.pdfTexto completo (inglês)application/pdf503137http://www.lume.ufrgs.br/bitstream/10183/100119/1/000921675.pdfa064e253fe8e929a397170fd1b2f3f6bMD51TEXT000921675.pdf.txt000921675.pdf.txtExtracted Texttext/plain24348http://www.lume.ufrgs.br/bitstream/10183/100119/2/000921675.pdf.txtabd0b39d6ad2872be95ec376357b66fcMD52THUMBNAIL000921675.pdf.jpg000921675.pdf.jpgGenerated Thumbnailimage/jpeg1696http://www.lume.ufrgs.br/bitstream/10183/100119/3/000921675.pdf.jpgd5335be146d9b3a1d41b75ae5d27e2deMD5310183/1001192024-01-31 06:01:03.379836oai:www.lume.ufrgs.br:10183/100119Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-01-31T08:01:03Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
title |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
spellingShingle |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice Scapin, Diane Salmonella enteritidis Probiótico Lactobacillus acidophilus S. Enteritidis SE86 Probiotics Mice Lactobacillus acidophilus LA10 |
title_short |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
title_full |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
title_fullStr |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
title_full_unstemmed |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
title_sort |
Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice |
author |
Scapin, Diane |
author_facet |
Scapin, Diane Grando, Williani Fabíola Rossi, Eliandra Mirlei Perez, Karla Joseane Malheiros, Patricia da Silva Tondo, Eduardo Cesar |
author_role |
author |
author2 |
Grando, Williani Fabíola Rossi, Eliandra Mirlei Perez, Karla Joseane Malheiros, Patricia da Silva Tondo, Eduardo Cesar |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Scapin, Diane Grando, Williani Fabíola Rossi, Eliandra Mirlei Perez, Karla Joseane Malheiros, Patricia da Silva Tondo, Eduardo Cesar |
dc.subject.por.fl_str_mv |
Salmonella enteritidis Probiótico Lactobacillus acidophilus |
topic |
Salmonella enteritidis Probiótico Lactobacillus acidophilus S. Enteritidis SE86 Probiotics Mice Lactobacillus acidophilus LA10 |
dc.subject.eng.fl_str_mv |
S. Enteritidis SE86 Probiotics Mice Lactobacillus acidophilus LA10 |
description |
Salmonella enterica serovar Enteritidis is one of the main pathogens responsible for foodborne illness in Brazil. Probiotic bacteria can play a role in defense and recovery from enteropathogenic infections. In this study, the ability of Lactobacillus acidophilus LA10 to colonise and exert antagonistic effects in the gastrointestinal tract was tested before and during experimental infection in conventional mice contaminated with S. Enteritidis (SE86). A dose of 0.1 mL containing 108 viable cells of SE86 and L. acidophilus LA10 was orally administered by gavage to mice. The experiment was divided into groups. As a negative control, Group 1 was administered only sterile saline solution. As a positive control, Group 2 was administered only SE86. Group 3 was first administered SE86, and after 10 days, treated with L. acidophilus LA10. Group 4 was first administered L. acidophilus LA10, and after 10 days, challenged with SE86. The results demonstrated that a significant number of SE86 cells were able to colonize the gastrointestinal tract of mice, specifically in the colon and ileum. L. acidophilus LA10 demonstrated an antagonistic effect against SE86, with better results observed for Group 3 over Group 4. Thus, L. acidophilus LA10 shows potential antagonistic effects against S. Enteritidis SE86, especially if administered after infection. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-08-12T02:10:40Z |
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1517-8382 |
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000921675 |
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http://hdl.handle.net/10183/100119 |
dc.language.iso.fl_str_mv |
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language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo. Vol. 44, no. 1 (Jan./Mar. 2013), p. 57-61 |
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