Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370 |
Resumo: | Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20 °C is not effective to keep mycelial viability of this fungus. Cryopreservation at -75 °C is effective when sucrose is used in substrates and/or cryotubes. Without sucrose, cryopreservation at -75 °C is effective only when wheat grains are used. Physiological characteristic as mycelial colony diameter is negatively affected when potato dextrose agar is used and unaffected when wheat grain is used after two-year cryopreservation at -75 °C. The fungus genome does not show alteration after two-year cryopreservation at -75 °C. |
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Brazilian Journal of Microbiology |
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Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrosePreservationFreezeCereal grainMonosaccharideAgaricus blazeiAbstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20 °C is not effective to keep mycelial viability of this fungus. Cryopreservation at -75 °C is effective when sucrose is used in substrates and/or cryotubes. Without sucrose, cryopreservation at -75 °C is effective only when wheat grains are used. Physiological characteristic as mycelial colony diameter is negatively affected when potato dextrose agar is used and unaffected when wheat grain is used after two-year cryopreservation at -75 °C. The fungus genome does not show alteration after two-year cryopreservation at -75 °C.Sociedade Brasileira de Microbiologia2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370Brazilian Journal of Microbiology v.49 n.2 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2017.08.003info:eu-repo/semantics/openAccessZaghi Júnior,Lienine LuizLopes,Ana DanielaCordeiro,Fábio AparecidoColla,Itaruã MachriBertéli,Míria Benetati DelgadoValle,Juliana Silveira doLinde,Giani AndreaColauto,Nelson Barroseng2018-04-17T00:00:00Zoai:scielo:S1517-83822018000200370Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-04-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
title |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
spellingShingle |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose Zaghi Júnior,Lienine Luiz Preservation Freeze Cereal grain Monosaccharide Agaricus blazei |
title_short |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
title_full |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
title_fullStr |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
title_full_unstemmed |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
title_sort |
Cryopreservation at -75 °C of Agaricus subrufescens on wheat grains with sucrose |
author |
Zaghi Júnior,Lienine Luiz |
author_facet |
Zaghi Júnior,Lienine Luiz Lopes,Ana Daniela Cordeiro,Fábio Aparecido Colla,Itaruã Machri Bertéli,Míria Benetati Delgado Valle,Juliana Silveira do Linde,Giani Andrea Colauto,Nelson Barros |
author_role |
author |
author2 |
Lopes,Ana Daniela Cordeiro,Fábio Aparecido Colla,Itaruã Machri Bertéli,Míria Benetati Delgado Valle,Juliana Silveira do Linde,Giani Andrea Colauto,Nelson Barros |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Zaghi Júnior,Lienine Luiz Lopes,Ana Daniela Cordeiro,Fábio Aparecido Colla,Itaruã Machri Bertéli,Míria Benetati Delgado Valle,Juliana Silveira do Linde,Giani Andrea Colauto,Nelson Barros |
dc.subject.por.fl_str_mv |
Preservation Freeze Cereal grain Monosaccharide Agaricus blazei |
topic |
Preservation Freeze Cereal grain Monosaccharide Agaricus blazei |
description |
Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20 °C is not effective to keep mycelial viability of this fungus. Cryopreservation at -75 °C is effective when sucrose is used in substrates and/or cryotubes. Without sucrose, cryopreservation at -75 °C is effective only when wheat grains are used. Physiological characteristic as mycelial colony diameter is negatively affected when potato dextrose agar is used and unaffected when wheat grain is used after two-year cryopreservation at -75 °C. The fungus genome does not show alteration after two-year cryopreservation at -75 °C. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2017.08.003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.49 n.2 2018 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122209628848128 |