Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68

Detalhes bibliográficos
Autor(a) principal: Samadlouie,Hamid-Reza
Data de Publicação: 2014
Outros Autores: Hamidi-Esfahani,Zohreh, Alavi,Seyed-Mehdi, Varastegani,Boshra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200010
Resumo: The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.
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spelling Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68Mortierella alpinaarachidonic acidlipidgene expressionreal time PCRThe time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.Sociedade Brasileira de Microbiologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200010Brazilian Journal of Microbiology v.45 n.2 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000200010info:eu-repo/semantics/openAccessSamadlouie,Hamid-RezaHamidi-Esfahani,ZohrehAlavi,Seyed-MehdiVarastegani,Boshraeng2014-09-30T00:00:00Zoai:scielo:S1517-83822014000200010Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-09-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
title Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
spellingShingle Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
Samadlouie,Hamid-Reza
Mortierella alpina
arachidonic acid
lipid
gene expression
real time PCR
title_short Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
title_full Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
title_fullStr Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
title_full_unstemmed Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
title_sort Expression analysis for genes involved in arachidonic acid biosynthesis in Mortierella alpina CBS 754.68
author Samadlouie,Hamid-Reza
author_facet Samadlouie,Hamid-Reza
Hamidi-Esfahani,Zohreh
Alavi,Seyed-Mehdi
Varastegani,Boshra
author_role author
author2 Hamidi-Esfahani,Zohreh
Alavi,Seyed-Mehdi
Varastegani,Boshra
author2_role author
author
author
dc.contributor.author.fl_str_mv Samadlouie,Hamid-Reza
Hamidi-Esfahani,Zohreh
Alavi,Seyed-Mehdi
Varastegani,Boshra
dc.subject.por.fl_str_mv Mortierella alpina
arachidonic acid
lipid
gene expression
real time PCR
topic Mortierella alpina
arachidonic acid
lipid
gene expression
real time PCR
description The time courses for production of fungal biomass, lipid, phenolic and arachidonic acid (ARA) as well as expression of the genes involved in biosynthesis of ARA and lipid were examined in Mortierella alpina CBS 754.68. A significant increase in the arachidonic acid content in lipids that coincided with reduced levels of lipid was obtained. Reduced gene expression occurred presumably due to the steady reduction of carbon and nitrogen resources. However, these energy resources were inefficiently compensated by the breakdown of the accumulated lipids that in turn, induced up-regulated expression of the candidate genes. The results further indicated that the expression of the GLELO encoding gene is a rate-limiting step in the biosynthesis of ARA in the early growth phase.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000200010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.2 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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