Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019 |
Resumo: | Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates showed resistance to the antibiotics tested; however, seven of them presented increased values for sulfamethoxazole MICs. Ten isolates belonged to serotype 4b, two to serotype 1/2a and only one to serotype 1/2b. After digestion with ApaI and AscI, the strains were distributed in 3 different groups according to their profile. It was observed that the spatial or temporally unrelated strains exhibited similar PFGE profiles, indicating a possible clonal relationship among them. |
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Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, BrazilListeria monocytogenesserotypesantibiotic resistancegenotypic characterizationlisteriosisConsidering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates showed resistance to the antibiotics tested; however, seven of them presented increased values for sulfamethoxazole MICs. Ten isolates belonged to serotype 4b, two to serotype 1/2a and only one to serotype 1/2b. After digestion with ApaI and AscI, the strains were distributed in 3 different groups according to their profile. It was observed that the spatial or temporally unrelated strains exhibited similar PFGE profiles, indicating a possible clonal relationship among them.Sociedade Brasileira de Microbiologia2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019Brazilian Journal of Microbiology v.38 n.2 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000200019info:eu-repo/semantics/openAccessLemes-Marques,Eneida GonçalvesCruz,Cristina DuranteDestro,Maria Teresaeng2007-06-29T00:00:00Zoai:scielo:S1517-83822007000200019Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-06-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
title |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
spellingShingle |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil Lemes-Marques,Eneida Gonçalves Listeria monocytogenes serotypes antibiotic resistance genotypic characterization listeriosis |
title_short |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
title_full |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
title_fullStr |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
title_full_unstemmed |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
title_sort |
Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil |
author |
Lemes-Marques,Eneida Gonçalves |
author_facet |
Lemes-Marques,Eneida Gonçalves Cruz,Cristina Durante Destro,Maria Teresa |
author_role |
author |
author2 |
Cruz,Cristina Durante Destro,Maria Teresa |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Lemes-Marques,Eneida Gonçalves Cruz,Cristina Durante Destro,Maria Teresa |
dc.subject.por.fl_str_mv |
Listeria monocytogenes serotypes antibiotic resistance genotypic characterization listeriosis |
topic |
Listeria monocytogenes serotypes antibiotic resistance genotypic characterization listeriosis |
description |
Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates showed resistance to the antibiotics tested; however, seven of them presented increased values for sulfamethoxazole MICs. Ten isolates belonged to serotype 4b, two to serotype 1/2a and only one to serotype 1/2b. After digestion with ApaI and AscI, the strains were distributed in 3 different groups according to their profile. It was observed that the spatial or temporally unrelated strains exhibited similar PFGE profiles, indicating a possible clonal relationship among them. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822007000200019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.38 n.2 2007 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201048350720 |