The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012 |
Resumo: | An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7. Analysis, by two-dimensional electrophoresis, of the X. fastidiosa proteins (120 µg of total proteins) grown in the presence of Pêra callus extract revealed 414 differentially expressed protein spots when compared to the protein profile obtained in the absence of the extract. The system developed in this study improves the induction and analysis of differentially expressed proteins of X. fastidiosa, which may be involved in pathogenicity. |
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Brazilian Journal of Microbiology |
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The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract2DEcalluspathogenicityproteinXylella-citrus interactionAn in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7. Analysis, by two-dimensional electrophoresis, of the X. fastidiosa proteins (120 µg of total proteins) grown in the presence of Pêra callus extract revealed 414 differentially expressed protein spots when compared to the protein profile obtained in the absence of the extract. The system developed in this study improves the induction and analysis of differentially expressed proteins of X. fastidiosa, which may be involved in pathogenicity.Sociedade Brasileira de Microbiologia2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012Brazilian Journal of Microbiology v.35 n.3 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000200012info:eu-repo/semantics/openAccessBellato,Cláudia de M.Garcia,Andréia K.M.Mestrinelli,FabianaTsai,Siu M.Machado,Marcos A.Meinhardt,Lyndel W.eng2005-11-25T00:00:00Zoai:scielo:S1517-83822004000200012Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2005-11-25T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
title |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
spellingShingle |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract Bellato,Cláudia de M. 2DE callus pathogenicity protein Xylella-citrus interaction |
title_short |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
title_full |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
title_fullStr |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
title_full_unstemmed |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
title_sort |
The induction of differentially expressed proteins of Xylella fastidiosa with citrus extract |
author |
Bellato,Cláudia de M. |
author_facet |
Bellato,Cláudia de M. Garcia,Andréia K.M. Mestrinelli,Fabiana Tsai,Siu M. Machado,Marcos A. Meinhardt,Lyndel W. |
author_role |
author |
author2 |
Garcia,Andréia K.M. Mestrinelli,Fabiana Tsai,Siu M. Machado,Marcos A. Meinhardt,Lyndel W. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bellato,Cláudia de M. Garcia,Andréia K.M. Mestrinelli,Fabiana Tsai,Siu M. Machado,Marcos A. Meinhardt,Lyndel W. |
dc.subject.por.fl_str_mv |
2DE callus pathogenicity protein Xylella-citrus interaction |
topic |
2DE callus pathogenicity protein Xylella-citrus interaction |
description |
An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7. Analysis, by two-dimensional electrophoresis, of the X. fastidiosa proteins (120 µg of total proteins) grown in the presence of Pêra callus extract revealed 414 differentially expressed protein spots when compared to the protein profile obtained in the absence of the extract. The system developed in this study improves the induction and analysis of differentially expressed proteins of X. fastidiosa, which may be involved in pathogenicity. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822004000200012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.35 n.3 2004 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200122458112 |