Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 http://hdl.handle.net/11449/1081 |
Resumo: | Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. |
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Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysisCitrusMicroarrayXylella fastidiosaXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.Ministry of Culture of Uruguay through the Program for Technical Development (PDT)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)National Institute of Agronomic Research of Uruguay (INIA)INIA, Estn Expt Wilson Ferreira Aldunate, Unidad Biotecnol, Rincon Del Colorado, UruguayUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Lab Bioquim Microrganismos & Plantas, Jaboticabal, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Mandioca & Fruticultura, Nucl Tecnol Recursos Gen & Desenvolvimento Varien, Cruz Das Almas, BA, BrazilEstacao Expt Citricultura, São Paulo, BrazilUniv São Paulo, Escola Super Agr Luiz de Queiroz, São Paulo, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Fac Ciencias Vet, Dept Engn Florestal, Diamantina, MG, BrazilCtr Citricultura Sylvio Moreira APTA, Cordeiropolis, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agropecuaria, Jaboticabal, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Lab Bioquim Microrganismos & Plantas, Jaboticabal, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agropecuaria, Jaboticabal, BrazilUniversidade Católica de ValparaísoINIAUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Estacao Expt CitriculturaUniversidade de São Paulo (USP)Univ Fed Vales Jequitinhonha & MucuriAgência Paulista de Tecnologia dos Agronegócios (APTA)Teresa Federici, Maria [UNESP]Marcondes, Jackson A. [UNESP]Picchi, Simone Cristina [UNESP]Stuchi, Eduardo S.Fadel, Andre LuizLaia, Marcelo L.Lemos, Manoel Victor F. [UNESP]Macedo Lemos, Eliana Gertrudes [UNESP]2014-05-20T13:13:13Z2014-05-20T13:13:13Z2012-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article33http://dx.doi.org/10.2225/vol15-issue3-fulltext-4Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 3, p. 33, 2012.0717-3458http://hdl.handle.net/11449/108110.2225/vol15-issue3-fulltext-4WOS:000307004200004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengElectronic Journal of Biotechnology1.8810,537info:eu-repo/semantics/openAccess2021-10-23T01:35:36Zoai:repositorio.unesp.br:11449/1081Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T01:35:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
title |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
spellingShingle |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis Teresa Federici, Maria [UNESP] Citrus Microarray Xylella fastidiosa |
title_short |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
title_full |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
title_fullStr |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
title_full_unstemmed |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
title_sort |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis |
author |
Teresa Federici, Maria [UNESP] |
author_facet |
Teresa Federici, Maria [UNESP] Marcondes, Jackson A. [UNESP] Picchi, Simone Cristina [UNESP] Stuchi, Eduardo S. Fadel, Andre Luiz Laia, Marcelo L. Lemos, Manoel Victor F. [UNESP] Macedo Lemos, Eliana Gertrudes [UNESP] |
author_role |
author |
author2 |
Marcondes, Jackson A. [UNESP] Picchi, Simone Cristina [UNESP] Stuchi, Eduardo S. Fadel, Andre Luiz Laia, Marcelo L. Lemos, Manoel Victor F. [UNESP] Macedo Lemos, Eliana Gertrudes [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
INIA Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Estacao Expt Citricultura Universidade de São Paulo (USP) Univ Fed Vales Jequitinhonha & Mucuri Agência Paulista de Tecnologia dos Agronegócios (APTA) |
dc.contributor.author.fl_str_mv |
Teresa Federici, Maria [UNESP] Marcondes, Jackson A. [UNESP] Picchi, Simone Cristina [UNESP] Stuchi, Eduardo S. Fadel, Andre Luiz Laia, Marcelo L. Lemos, Manoel Victor F. [UNESP] Macedo Lemos, Eliana Gertrudes [UNESP] |
dc.subject.por.fl_str_mv |
Citrus Microarray Xylella fastidiosa |
topic |
Citrus Microarray Xylella fastidiosa |
description |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-15 2014-05-20T13:13:13Z 2014-05-20T13:13:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 3, p. 33, 2012. 0717-3458 http://hdl.handle.net/11449/1081 10.2225/vol15-issue3-fulltext-4 WOS:000307004200004 |
url |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 http://hdl.handle.net/11449/1081 |
identifier_str_mv |
Electronic Journal of Biotechnology. Valparaiso: Univ Catolica de Valparaiso, v. 15, n. 3, p. 33, 2012. 0717-3458 10.2225/vol15-issue3-fulltext-4 WOS:000307004200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Electronic Journal of Biotechnology 1.881 0,537 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
33 |
dc.publisher.none.fl_str_mv |
Universidade Católica de Valparaíso |
publisher.none.fl_str_mv |
Universidade Católica de Valparaíso |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964384506675200 |