Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files

Detalhes bibliográficos
Autor(a) principal: Silva,Raquel Assed Bezerra da
Data de Publicação: 2007
Outros Autores: Leonardo,Mário Roberto, Faccioli,Lúcia Helena, Medeiros,Alexandra Ivo, Nelson-Filho,Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200015
Resumo: This study evaluated the presence of lipopolysaccharide (LPS) in endodontic files after in vitro contamination with E. coli LPS and sterilization in dry heat or wet heat, with or without previous immersion in a Ca(OH)2 suspension. LPS was quantified using the Kinetic QCL TM test and data were analyzed statistically by the t-test. LPS was quantified only in the contaminated group, not submitted to any immersion or sterilization procedure (0.262±0.1296 endotoxin units/mL) (p=0.0003). In conclusion, both wet heat and dry heat sterilization were effective in inactivating LPS without the need of previous immersion of the files in a Ca(OH)2 suspension.
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spelling Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic filesendotoxinLPSEndodonticsdry heatwet heatThis study evaluated the presence of lipopolysaccharide (LPS) in endodontic files after in vitro contamination with E. coli LPS and sterilization in dry heat or wet heat, with or without previous immersion in a Ca(OH)2 suspension. LPS was quantified using the Kinetic QCL TM test and data were analyzed statistically by the t-test. LPS was quantified only in the contaminated group, not submitted to any immersion or sterilization procedure (0.262±0.1296 endotoxin units/mL) (p=0.0003). In conclusion, both wet heat and dry heat sterilization were effective in inactivating LPS without the need of previous immersion of the files in a Ca(OH)2 suspension.Sociedade Brasileira de Microbiologia2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200015Brazilian Journal of Microbiology v.38 n.2 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000200015info:eu-repo/semantics/openAccessSilva,Raquel Assed Bezerra daLeonardo,Mário RobertoFaccioli,Lúcia HelenaMedeiros,Alexandra IvoNelson-Filho,Pauloeng2007-06-29T00:00:00Zoai:scielo:S1517-83822007000200015Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-06-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
title Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
spellingShingle Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
Silva,Raquel Assed Bezerra da
endotoxin
LPS
Endodontics
dry heat
wet heat
title_short Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
title_full Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
title_fullStr Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
title_full_unstemmed Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
title_sort Effect of different methods of sterilization on the inactivation of bacterial endotoxin (LPS) in endodontic files
author Silva,Raquel Assed Bezerra da
author_facet Silva,Raquel Assed Bezerra da
Leonardo,Mário Roberto
Faccioli,Lúcia Helena
Medeiros,Alexandra Ivo
Nelson-Filho,Paulo
author_role author
author2 Leonardo,Mário Roberto
Faccioli,Lúcia Helena
Medeiros,Alexandra Ivo
Nelson-Filho,Paulo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva,Raquel Assed Bezerra da
Leonardo,Mário Roberto
Faccioli,Lúcia Helena
Medeiros,Alexandra Ivo
Nelson-Filho,Paulo
dc.subject.por.fl_str_mv endotoxin
LPS
Endodontics
dry heat
wet heat
topic endotoxin
LPS
Endodontics
dry heat
wet heat
description This study evaluated the presence of lipopolysaccharide (LPS) in endodontic files after in vitro contamination with E. coli LPS and sterilization in dry heat or wet heat, with or without previous immersion in a Ca(OH)2 suspension. LPS was quantified using the Kinetic QCL TM test and data were analyzed statistically by the t-test. LPS was quantified only in the contaminated group, not submitted to any immersion or sterilization procedure (0.262±0.1296 endotoxin units/mL) (p=0.0003). In conclusion, both wet heat and dry heat sterilization were effective in inactivating LPS without the need of previous immersion of the files in a Ca(OH)2 suspension.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.2 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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