Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Ramos,Cíntia Lacerda
Data de Publicação: 2013
Outros Autores: Duarte,Whasley Ferreira, Freire,Ana Luiza, Dias,Disney Ribeiro, Eleutherio,Elis Cristina Araújo, Schwan,Rosane Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300044
Resumo: Sixty six indigenous Saccharomyces cerevisiae strains were evaluated in stressful conditions (temperature, osmolarity, sulphite and ethanol tolerance) and also ability to flocculate. Eighteen strains showed tolerant characteristics to these stressful conditions, growing at 42 ºC, in 0.04% sulphite, 1 mol L-1 NaCl and 12% ethanol. No flocculent characteristics were observed. These strains were evaluated according to their fermentative performance in sugar cane juice. The conversion factors of substrates into ethanol (Yp/s), glycerol (Yg/s) and acetic acid (Yac/s), were calculated. The highest values of Yp/s in sugar cane juice fermentation were obtained by four strains, one isolated from fruit (0.46) and the others from sugar cane (0.45, 0.44 and 0.43). These values were higher than the value obtained using traditional yeast (0.38) currently employed in the Brazilian bioethanol industry. The parameters Yg/s and Yac/s were low for all strains. The UFLA FW221 presented the higher values for parameter related to bioethanol production. Thus, it was tested in co-culture with Lactobacillus fermentum. Besides this, a 20-L vessel for five consecutive batches of fermentation was performed. This strain was genetically stable and remained viable during all batches, producing high amounts of ethanol. The UFLA FW221 isolated from fruit was suitable to produce bioethanol in sugar cane juice. Therefore, the study of the biodiversity of yeasts from different environmental can reveal strains with desired characteristics to industrial applications.
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spelling Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiaealcoholic fermentationbiofuelfermentation kineticsUFLA FW221Saccharomyces cerevisiaeSixty six indigenous Saccharomyces cerevisiae strains were evaluated in stressful conditions (temperature, osmolarity, sulphite and ethanol tolerance) and also ability to flocculate. Eighteen strains showed tolerant characteristics to these stressful conditions, growing at 42 ºC, in 0.04% sulphite, 1 mol L-1 NaCl and 12% ethanol. No flocculent characteristics were observed. These strains were evaluated according to their fermentative performance in sugar cane juice. The conversion factors of substrates into ethanol (Yp/s), glycerol (Yg/s) and acetic acid (Yac/s), were calculated. The highest values of Yp/s in sugar cane juice fermentation were obtained by four strains, one isolated from fruit (0.46) and the others from sugar cane (0.45, 0.44 and 0.43). These values were higher than the value obtained using traditional yeast (0.38) currently employed in the Brazilian bioethanol industry. The parameters Yg/s and Yac/s were low for all strains. The UFLA FW221 presented the higher values for parameter related to bioethanol production. Thus, it was tested in co-culture with Lactobacillus fermentum. Besides this, a 20-L vessel for five consecutive batches of fermentation was performed. This strain was genetically stable and remained viable during all batches, producing high amounts of ethanol. The UFLA FW221 isolated from fruit was suitable to produce bioethanol in sugar cane juice. Therefore, the study of the biodiversity of yeasts from different environmental can reveal strains with desired characteristics to industrial applications.Sociedade Brasileira de Microbiologia2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300044Brazilian Journal of Microbiology v.44 n.3 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013005000051info:eu-repo/semantics/openAccessRamos,Cíntia LacerdaDuarte,Whasley FerreiraFreire,Ana LuizaDias,Disney RibeiroEleutherio,Elis Cristina AraújoSchwan,Rosane Freitaseng2014-02-03T00:00:00Zoai:scielo:S1517-83822013000300044Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-02-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
title Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
spellingShingle Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
Ramos,Cíntia Lacerda
alcoholic fermentation
biofuel
fermentation kinetics
UFLA FW221
Saccharomyces cerevisiae
title_short Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
title_full Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
title_fullStr Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
title_full_unstemmed Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
title_sort Evaluation of stress tolerance and fermentative behavior of indigenous Saccharomyces cerevisiae
author Ramos,Cíntia Lacerda
author_facet Ramos,Cíntia Lacerda
Duarte,Whasley Ferreira
Freire,Ana Luiza
Dias,Disney Ribeiro
Eleutherio,Elis Cristina Araújo
Schwan,Rosane Freitas
author_role author
author2 Duarte,Whasley Ferreira
Freire,Ana Luiza
Dias,Disney Ribeiro
Eleutherio,Elis Cristina Araújo
Schwan,Rosane Freitas
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos,Cíntia Lacerda
Duarte,Whasley Ferreira
Freire,Ana Luiza
Dias,Disney Ribeiro
Eleutherio,Elis Cristina Araújo
Schwan,Rosane Freitas
dc.subject.por.fl_str_mv alcoholic fermentation
biofuel
fermentation kinetics
UFLA FW221
Saccharomyces cerevisiae
topic alcoholic fermentation
biofuel
fermentation kinetics
UFLA FW221
Saccharomyces cerevisiae
description Sixty six indigenous Saccharomyces cerevisiae strains were evaluated in stressful conditions (temperature, osmolarity, sulphite and ethanol tolerance) and also ability to flocculate. Eighteen strains showed tolerant characteristics to these stressful conditions, growing at 42 ºC, in 0.04% sulphite, 1 mol L-1 NaCl and 12% ethanol. No flocculent characteristics were observed. These strains were evaluated according to their fermentative performance in sugar cane juice. The conversion factors of substrates into ethanol (Yp/s), glycerol (Yg/s) and acetic acid (Yac/s), were calculated. The highest values of Yp/s in sugar cane juice fermentation were obtained by four strains, one isolated from fruit (0.46) and the others from sugar cane (0.45, 0.44 and 0.43). These values were higher than the value obtained using traditional yeast (0.38) currently employed in the Brazilian bioethanol industry. The parameters Yg/s and Yac/s were low for all strains. The UFLA FW221 presented the higher values for parameter related to bioethanol production. Thus, it was tested in co-culture with Lactobacillus fermentum. Besides this, a 20-L vessel for five consecutive batches of fermentation was performed. This strain was genetically stable and remained viable during all batches, producing high amounts of ethanol. The UFLA FW221 isolated from fruit was suitable to produce bioethanol in sugar cane juice. Therefore, the study of the biodiversity of yeasts from different environmental can reveal strains with desired characteristics to industrial applications.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300044
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300044
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822013005000051
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.44 n.3 2013
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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