Detection and characterization of bacteriocin-producing Lactococcus lactis strains

Detalhes bibliográficos
Autor(a) principal: Moreno,Izildinha
Data de Publicação: 1999
Outros Autores: Lerayer,Alda L.S., Leitão,Mauro F. de Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista de Microbiologia
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200008
Resumo: One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac+) and proteinase-negative (Prt-). The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. Four strains (ITAL 435, ITAL 436, ITAL 437 and ITAL 438) showed identical profiles and the other were quite distinct.
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spelling Detection and characterization of bacteriocin-producing Lactococcus lactis strainsL. lactisbacteriocinsfermentationplasmidsOne hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac+) and proteinase-negative (Prt-). The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. Four strains (ITAL 435, ITAL 436, ITAL 437 and ITAL 438) showed identical profiles and the other were quite distinct.Sociedade Brasileira de Microbiologia1999-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200008Revista de Microbiologia v.30 n.2 1999reponame:Revista de Microbiologiainstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S0001-37141999000200008info:eu-repo/semantics/openAccessMoreno,IzildinhaLerayer,Alda L.S.Leitão,Mauro F. de Freitaseng2000-01-06T00:00:00Zoai:scielo:S0001-37141999000200008Revistahttps://www.scielo.br/j/rm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||revmicro@icb.usp.br0001-37140001-3714opendoar:2000-01-06T00:00Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Detection and characterization of bacteriocin-producing Lactococcus lactis strains
title Detection and characterization of bacteriocin-producing Lactococcus lactis strains
spellingShingle Detection and characterization of bacteriocin-producing Lactococcus lactis strains
Moreno,Izildinha
L. lactis
bacteriocins
fermentation
plasmids
title_short Detection and characterization of bacteriocin-producing Lactococcus lactis strains
title_full Detection and characterization of bacteriocin-producing Lactococcus lactis strains
title_fullStr Detection and characterization of bacteriocin-producing Lactococcus lactis strains
title_full_unstemmed Detection and characterization of bacteriocin-producing Lactococcus lactis strains
title_sort Detection and characterization of bacteriocin-producing Lactococcus lactis strains
author Moreno,Izildinha
author_facet Moreno,Izildinha
Lerayer,Alda L.S.
Leitão,Mauro F. de Freitas
author_role author
author2 Lerayer,Alda L.S.
Leitão,Mauro F. de Freitas
author2_role author
author
dc.contributor.author.fl_str_mv Moreno,Izildinha
Lerayer,Alda L.S.
Leitão,Mauro F. de Freitas
dc.subject.por.fl_str_mv L. lactis
bacteriocins
fermentation
plasmids
topic L. lactis
bacteriocins
fermentation
plasmids
description One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin-producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac+) and proteinase-negative (Prt-). The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. Four strains (ITAL 435, ITAL 436, ITAL 437 and ITAL 438) showed identical profiles and the other were quite distinct.
publishDate 1999
dc.date.none.fl_str_mv 1999-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0001-37141999000200008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Revista de Microbiologia v.30 n.2 1999
reponame:Revista de Microbiologia
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Revista de Microbiologia
collection Revista de Microbiologia
repository.name.fl_str_mv Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||revmicro@icb.usp.br
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