Characterization of bacteriocins produced by Lactococcus lactis strains

Detalhes bibliográficos
Autor(a) principal: Moreno,Izildinha
Data de Publicação: 2000
Outros Autores: Lerayer,Alda L.S., Baldini,Vera L.S., Leitão,Mauro F. de F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300007
Resumo: Bacteriocins produced by fifteen strains of Lactococcus lactis (14 L. lactis subsp. lactis and one L. lactis subsp. cremoris) were heat resistant, sensitive to several proteolytic enzymes and active over a wide range of pH. Their resistance to the heating was greatly influenced by the pH. Only the strain L. lactis subsp. lactis ITAL 383 produced a bacteriocin with a wide activity spectrum, similar to nisin of L. lactis subsp. lactis ATCC 11454. This bacteriocin inhibited closely related species and other Gram-positive microorganisms including Listeria monocytogenes and Staphylococcus aureus, but it was not active against the Gram-negative bacteria tested. The identification of partially purified antimicrobial compounds by SDS-PAGE showed that bacteriocin produced by strain ITAL 383 had the same molecular weight of nisin produced by L. lactis subsp. lactis ATCC 11454.
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spelling Characterization of bacteriocins produced by Lactococcus lactis strainsbacteriocinsL. lactisactivity spectrumphysical-chemical characteristicBacteriocins produced by fifteen strains of Lactococcus lactis (14 L. lactis subsp. lactis and one L. lactis subsp. cremoris) were heat resistant, sensitive to several proteolytic enzymes and active over a wide range of pH. Their resistance to the heating was greatly influenced by the pH. Only the strain L. lactis subsp. lactis ITAL 383 produced a bacteriocin with a wide activity spectrum, similar to nisin of L. lactis subsp. lactis ATCC 11454. This bacteriocin inhibited closely related species and other Gram-positive microorganisms including Listeria monocytogenes and Staphylococcus aureus, but it was not active against the Gram-negative bacteria tested. The identification of partially purified antimicrobial compounds by SDS-PAGE showed that bacteriocin produced by strain ITAL 383 had the same molecular weight of nisin produced by L. lactis subsp. lactis ATCC 11454.Sociedade Brasileira de Microbiologia2000-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300007Brazilian Journal of Microbiology v.31 n.3 2000reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822000000300007info:eu-repo/semantics/openAccessMoreno,IzildinhaLerayer,Alda L.S.Baldini,Vera L.S.Leitão,Mauro F. de F.eng2001-03-16T00:00:00Zoai:scielo:S1517-83822000000300007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2001-03-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Characterization of bacteriocins produced by Lactococcus lactis strains
title Characterization of bacteriocins produced by Lactococcus lactis strains
spellingShingle Characterization of bacteriocins produced by Lactococcus lactis strains
Moreno,Izildinha
bacteriocins
L. lactis
activity spectrum
physical-chemical characteristic
title_short Characterization of bacteriocins produced by Lactococcus lactis strains
title_full Characterization of bacteriocins produced by Lactococcus lactis strains
title_fullStr Characterization of bacteriocins produced by Lactococcus lactis strains
title_full_unstemmed Characterization of bacteriocins produced by Lactococcus lactis strains
title_sort Characterization of bacteriocins produced by Lactococcus lactis strains
author Moreno,Izildinha
author_facet Moreno,Izildinha
Lerayer,Alda L.S.
Baldini,Vera L.S.
Leitão,Mauro F. de F.
author_role author
author2 Lerayer,Alda L.S.
Baldini,Vera L.S.
Leitão,Mauro F. de F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Moreno,Izildinha
Lerayer,Alda L.S.
Baldini,Vera L.S.
Leitão,Mauro F. de F.
dc.subject.por.fl_str_mv bacteriocins
L. lactis
activity spectrum
physical-chemical characteristic
topic bacteriocins
L. lactis
activity spectrum
physical-chemical characteristic
description Bacteriocins produced by fifteen strains of Lactococcus lactis (14 L. lactis subsp. lactis and one L. lactis subsp. cremoris) were heat resistant, sensitive to several proteolytic enzymes and active over a wide range of pH. Their resistance to the heating was greatly influenced by the pH. Only the strain L. lactis subsp. lactis ITAL 383 produced a bacteriocin with a wide activity spectrum, similar to nisin of L. lactis subsp. lactis ATCC 11454. This bacteriocin inhibited closely related species and other Gram-positive microorganisms including Listeria monocytogenes and Staphylococcus aureus, but it was not active against the Gram-negative bacteria tested. The identification of partially purified antimicrobial compounds by SDS-PAGE showed that bacteriocin produced by strain ITAL 383 had the same molecular weight of nisin produced by L. lactis subsp. lactis ATCC 11454.
publishDate 2000
dc.date.none.fl_str_mv 2000-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822000000300007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.31 n.3 2000
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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