Numerical analysis of impedance spectra of yeast suspensions
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Microwaves. Optoelectronics and Electromagnetic Applications |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032 |
Resumo: | Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m. |
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Journal of Microwaves. Optoelectronics and Electromagnetic Applications |
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Numerical analysis of impedance spectra of yeast suspensionsElectroporationGenetic Algorithmimpedance spectrumyeast cell suspensionAnalysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m.Sociedade Brasileira de Microondas e Optoeletrônica e Sociedade Brasileira de Eletromagnetismo2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032Journal of Microwaves, Optoelectronics and Electromagnetic Applications v.12 n.2 2013reponame:Journal of Microwaves. Optoelectronics and Electromagnetic Applicationsinstname:Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)instacron:SBMO10.1590/S2179-10742013000200032info:eu-repo/semantics/openAccessRamos,AirtonFarias D.,Hericeng2014-02-10T00:00:00Zoai:scielo:S2179-10742013000200032Revistahttp://www.jmoe.org/index.php/jmoe/indexONGhttps://old.scielo.br/oai/scielo-oai.php||editor_jmoe@sbmo.org.br2179-10742179-1074opendoar:2014-02-10T00:00Journal of Microwaves. Optoelectronics and Electromagnetic Applications - Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)false |
dc.title.none.fl_str_mv |
Numerical analysis of impedance spectra of yeast suspensions |
title |
Numerical analysis of impedance spectra of yeast suspensions |
spellingShingle |
Numerical analysis of impedance spectra of yeast suspensions Ramos,Airton Electroporation Genetic Algorithm impedance spectrum yeast cell suspension |
title_short |
Numerical analysis of impedance spectra of yeast suspensions |
title_full |
Numerical analysis of impedance spectra of yeast suspensions |
title_fullStr |
Numerical analysis of impedance spectra of yeast suspensions |
title_full_unstemmed |
Numerical analysis of impedance spectra of yeast suspensions |
title_sort |
Numerical analysis of impedance spectra of yeast suspensions |
author |
Ramos,Airton |
author_facet |
Ramos,Airton Farias D.,Heric |
author_role |
author |
author2 |
Farias D.,Heric |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Ramos,Airton Farias D.,Heric |
dc.subject.por.fl_str_mv |
Electroporation Genetic Algorithm impedance spectrum yeast cell suspension |
topic |
Electroporation Genetic Algorithm impedance spectrum yeast cell suspension |
description |
Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S2179-10742013000200032 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microondas e Optoeletrônica e Sociedade Brasileira de Eletromagnetismo |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microondas e Optoeletrônica e Sociedade Brasileira de Eletromagnetismo |
dc.source.none.fl_str_mv |
Journal of Microwaves, Optoelectronics and Electromagnetic Applications v.12 n.2 2013 reponame:Journal of Microwaves. Optoelectronics and Electromagnetic Applications instname:Sociedade Brasileira de Microondas e Optoeletrônica (SBMO) instacron:SBMO |
instname_str |
Sociedade Brasileira de Microondas e Optoeletrônica (SBMO) |
instacron_str |
SBMO |
institution |
SBMO |
reponame_str |
Journal of Microwaves. Optoelectronics and Electromagnetic Applications |
collection |
Journal of Microwaves. Optoelectronics and Electromagnetic Applications |
repository.name.fl_str_mv |
Journal of Microwaves. Optoelectronics and Electromagnetic Applications - Sociedade Brasileira de Microondas e Optoeletrônica (SBMO) |
repository.mail.fl_str_mv |
||editor_jmoe@sbmo.org.br |
_version_ |
1752122125742768128 |