Numerical analysis of impedance spectra of yeast suspensions

Detalhes bibliográficos
Autor(a) principal: Ramos,Airton
Data de Publicação: 2013
Outros Autores: Farias D.,Heric
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of Microwaves. Optoelectronics and Electromagnetic Applications
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032
Resumo: Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m.
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spelling Numerical analysis of impedance spectra of yeast suspensionsElectroporationGenetic Algorithmimpedance spectrumyeast cell suspensionAnalysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m.Sociedade Brasileira de Microondas e Optoeletrônica e Sociedade Brasileira de Eletromagnetismo2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032Journal of Microwaves, Optoelectronics and Electromagnetic Applications v.12 n.2 2013reponame:Journal of Microwaves. Optoelectronics and Electromagnetic Applicationsinstname:Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)instacron:SBMO10.1590/S2179-10742013000200032info:eu-repo/semantics/openAccessRamos,AirtonFarias D.,Hericeng2014-02-10T00:00:00Zoai:scielo:S2179-10742013000200032Revistahttp://www.jmoe.org/index.php/jmoe/indexONGhttps://old.scielo.br/oai/scielo-oai.php||editor_jmoe@sbmo.org.br2179-10742179-1074opendoar:2014-02-10T00:00Journal of Microwaves. Optoelectronics and Electromagnetic Applications - Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)false
dc.title.none.fl_str_mv Numerical analysis of impedance spectra of yeast suspensions
title Numerical analysis of impedance spectra of yeast suspensions
spellingShingle Numerical analysis of impedance spectra of yeast suspensions
Ramos,Airton
Electroporation
Genetic Algorithm
impedance spectrum
yeast cell suspension
title_short Numerical analysis of impedance spectra of yeast suspensions
title_full Numerical analysis of impedance spectra of yeast suspensions
title_fullStr Numerical analysis of impedance spectra of yeast suspensions
title_full_unstemmed Numerical analysis of impedance spectra of yeast suspensions
title_sort Numerical analysis of impedance spectra of yeast suspensions
author Ramos,Airton
author_facet Ramos,Airton
Farias D.,Heric
author_role author
author2 Farias D.,Heric
author2_role author
dc.contributor.author.fl_str_mv Ramos,Airton
Farias D.,Heric
dc.subject.por.fl_str_mv Electroporation
Genetic Algorithm
impedance spectrum
yeast cell suspension
topic Electroporation
Genetic Algorithm
impedance spectrum
yeast cell suspension
description Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to experimental spectra of Kluyveromyces marxianus CBS 6556 yeasts suspended in distilled water. It was possible to determine the variation of conductivity of suspension avoiding the influence of electrode polarization and dielectric dispersion due to interfacial and diffusion polarization on the cell surface. It was observed that the conductivity in the range of 1 to 100 KHz increases 2.5 times with pulses of 400 KV/m.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-10742013000200032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S2179-10742013000200032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microondas e Optoeletrônica e Sociedade Brasileira de Eletromagnetismo
publisher.none.fl_str_mv Sociedade Brasileira de Microondas e Optoeletrônica e Sociedade Brasileira de Eletromagnetismo
dc.source.none.fl_str_mv Journal of Microwaves, Optoelectronics and Electromagnetic Applications v.12 n.2 2013
reponame:Journal of Microwaves. Optoelectronics and Electromagnetic Applications
instname:Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)
instacron:SBMO
instname_str Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)
instacron_str SBMO
institution SBMO
reponame_str Journal of Microwaves. Optoelectronics and Electromagnetic Applications
collection Journal of Microwaves. Optoelectronics and Electromagnetic Applications
repository.name.fl_str_mv Journal of Microwaves. Optoelectronics and Electromagnetic Applications - Sociedade Brasileira de Microondas e Optoeletrônica (SBMO)
repository.mail.fl_str_mv ||editor_jmoe@sbmo.org.br
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