Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000100001 |
Resumo: | INTRODUCTION: The present study investigated the association between mannose-binding lectin (MBL) gene polymorphism and serum levels with infection by HIV-1. METHODS: Blood samples (5mL) were collected from 97 HIV-1-infected individuals resident in Belém, State of Pará, Brazil, who attended the Special Outpatient Unit for Infections and Parasitic Diseases (URE-DIPE). CD4+ T-lymphocyte count and plasma viral load were quantified. A 349bp fragment of exon 1 of the MBL was amplified via PCR, using genomic DNA extracted from controls and HIV-1-infected individuals, following established protocols. MBL plasma levels of the patients were quantified using an enzyme immunoassay kit. RESULTS: Two alleles were observed: MBL*O, with a frequency of 26.3% in HIV-1-infected individuals; and the wild allele MBL*A (73.7%). Similar frequencies were observed in the control group (p > 0.05). Genotype frequencies were distributed according to the Hardy-Weinberg equilibrium in both groups. Mean MBL plasma levels varied by genotype, with statistically significant differences between the AA and AO (p < 0.0001), and AA and OO (p < 0.001) genotypes, but not AO and OO (p = 0.17). Additionally, CD4+ T-lymphocytes and plasma viral load levels did not differ significantly by genotype (p > 0.05). CONCLUSIONS: The results of this study do not support the hypothesis that MBL gene polymorphism or low plasma MBL concentrations might have a direct influence on HIV-1 infection, although a broader study involving a large number of patients is needed. |
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Revista da Sociedade Brasileira de Medicina Tropical |
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Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individualsHIV-1MBL gene polymorphismAmazon regionINTRODUCTION: The present study investigated the association between mannose-binding lectin (MBL) gene polymorphism and serum levels with infection by HIV-1. METHODS: Blood samples (5mL) were collected from 97 HIV-1-infected individuals resident in Belém, State of Pará, Brazil, who attended the Special Outpatient Unit for Infections and Parasitic Diseases (URE-DIPE). CD4+ T-lymphocyte count and plasma viral load were quantified. A 349bp fragment of exon 1 of the MBL was amplified via PCR, using genomic DNA extracted from controls and HIV-1-infected individuals, following established protocols. MBL plasma levels of the patients were quantified using an enzyme immunoassay kit. RESULTS: Two alleles were observed: MBL*O, with a frequency of 26.3% in HIV-1-infected individuals; and the wild allele MBL*A (73.7%). Similar frequencies were observed in the control group (p > 0.05). Genotype frequencies were distributed according to the Hardy-Weinberg equilibrium in both groups. Mean MBL plasma levels varied by genotype, with statistically significant differences between the AA and AO (p < 0.0001), and AA and OO (p < 0.001) genotypes, but not AO and OO (p = 0.17). Additionally, CD4+ T-lymphocytes and plasma viral load levels did not differ significantly by genotype (p > 0.05). CONCLUSIONS: The results of this study do not support the hypothesis that MBL gene polymorphism or low plasma MBL concentrations might have a direct influence on HIV-1 infection, although a broader study involving a large number of patients is needed.Sociedade Brasileira de Medicina Tropical - SBMT2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000100001Revista da Sociedade Brasileira de Medicina Tropical v.44 n.1 2011reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822011000100001info:eu-repo/semantics/openAccessVallinoto,Antonio Carlos RosárioFreitas,Felipe BonfimGuirelli,IsabellaMachado,Luiz Fernando AlmeidaAzevedo,Vânia NakauthCayres-Vallinoto,IzauraIshak,Marluísa Oliveira GuimarãesIshak,Ricardoeng2011-02-14T00:00:00Zoai:scielo:S0037-86822011000100001Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2011-02-14T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
title |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
spellingShingle |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals Vallinoto,Antonio Carlos Rosário HIV-1 MBL gene polymorphism Amazon region |
title_short |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
title_full |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
title_fullStr |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
title_full_unstemmed |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
title_sort |
Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals |
author |
Vallinoto,Antonio Carlos Rosário |
author_facet |
Vallinoto,Antonio Carlos Rosário Freitas,Felipe Bonfim Guirelli,Isabella Machado,Luiz Fernando Almeida Azevedo,Vânia Nakauth Cayres-Vallinoto,Izaura Ishak,Marluísa Oliveira Guimarães Ishak,Ricardo |
author_role |
author |
author2 |
Freitas,Felipe Bonfim Guirelli,Isabella Machado,Luiz Fernando Almeida Azevedo,Vânia Nakauth Cayres-Vallinoto,Izaura Ishak,Marluísa Oliveira Guimarães Ishak,Ricardo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vallinoto,Antonio Carlos Rosário Freitas,Felipe Bonfim Guirelli,Isabella Machado,Luiz Fernando Almeida Azevedo,Vânia Nakauth Cayres-Vallinoto,Izaura Ishak,Marluísa Oliveira Guimarães Ishak,Ricardo |
dc.subject.por.fl_str_mv |
HIV-1 MBL gene polymorphism Amazon region |
topic |
HIV-1 MBL gene polymorphism Amazon region |
description |
INTRODUCTION: The present study investigated the association between mannose-binding lectin (MBL) gene polymorphism and serum levels with infection by HIV-1. METHODS: Blood samples (5mL) were collected from 97 HIV-1-infected individuals resident in Belém, State of Pará, Brazil, who attended the Special Outpatient Unit for Infections and Parasitic Diseases (URE-DIPE). CD4+ T-lymphocyte count and plasma viral load were quantified. A 349bp fragment of exon 1 of the MBL was amplified via PCR, using genomic DNA extracted from controls and HIV-1-infected individuals, following established protocols. MBL plasma levels of the patients were quantified using an enzyme immunoassay kit. RESULTS: Two alleles were observed: MBL*O, with a frequency of 26.3% in HIV-1-infected individuals; and the wild allele MBL*A (73.7%). Similar frequencies were observed in the control group (p > 0.05). Genotype frequencies were distributed according to the Hardy-Weinberg equilibrium in both groups. Mean MBL plasma levels varied by genotype, with statistically significant differences between the AA and AO (p < 0.0001), and AA and OO (p < 0.001) genotypes, but not AO and OO (p = 0.17). Additionally, CD4+ T-lymphocytes and plasma viral load levels did not differ significantly by genotype (p > 0.05). CONCLUSIONS: The results of this study do not support the hypothesis that MBL gene polymorphism or low plasma MBL concentrations might have a direct influence on HIV-1 infection, although a broader study involving a large number of patients is needed. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000100001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822011000100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.44 n.1 2011 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122156258426880 |