QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000600013 |
Resumo: | "Quantitative Buffy Coat" (QBC®) is a direct and fast fluorescent method used for the identification of blood parasites. Since Leishmania chagasi circulates in blood, we decided to test it in American visceral leishmaniasis (AVL). Bone marrow (BM) and peripheral blood (PB) of 49 persons and PB of 31 dogs were analyzed. QBC® was positive in BM of 11/11 patients with AVL and in 1/6 patients with other diseases. Amastigotes were identified in PB of 18/22 patients with AVL and in none without AVL. The test was positive in 30 out of the 31 seropositive dogs and in 28/28 dogs with Leishmania identified in other tissues. QBC® is a promising method for diagnosis of human AVL, and possibly for the exam of PB of patients with AVL/AIDS, for the control of the cure and for the identification of asymptomatic carriers. Because it is fast and easy to collect and execute, QBC® should be evaluated for programs of reservoir control. |
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QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary dataKala-azarVisceral leishmaniasisLeishmania chagasiDogQBC®"Quantitative Buffy Coat" (QBC®) is a direct and fast fluorescent method used for the identification of blood parasites. Since Leishmania chagasi circulates in blood, we decided to test it in American visceral leishmaniasis (AVL). Bone marrow (BM) and peripheral blood (PB) of 49 persons and PB of 31 dogs were analyzed. QBC® was positive in BM of 11/11 patients with AVL and in 1/6 patients with other diseases. Amastigotes were identified in PB of 18/22 patients with AVL and in none without AVL. The test was positive in 30 out of the 31 seropositive dogs and in 28/28 dogs with Leishmania identified in other tissues. QBC® is a promising method for diagnosis of human AVL, and possibly for the exam of PB of patients with AVL/AIDS, for the control of the cure and for the identification of asymptomatic carriers. Because it is fast and easy to collect and execute, QBC® should be evaluated for programs of reservoir control.Sociedade Brasileira de Medicina Tropical - SBMT2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000600013Revista da Sociedade Brasileira de Medicina Tropical v.34 n.6 2001reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822001000600013info:eu-repo/semantics/openAccessLiarte,Daniel B.Mendonça,Ivete L.Luz,Francisco C.O.Abreu,Elza A.S. deMello,Gustavo W.S.Farias,Teresinha J.C.Ferreira,Afonso F.B.Millington,Maria A.Costa,Carlos H.N.eng2002-01-23T00:00:00Zoai:scielo:S0037-86822001000600013Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2002-01-23T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
title |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
spellingShingle |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data Liarte,Daniel B. Kala-azar Visceral leishmaniasis Leishmania chagasi Dog QBC® |
title_short |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
title_full |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
title_fullStr |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
title_full_unstemmed |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
title_sort |
QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data |
author |
Liarte,Daniel B. |
author_facet |
Liarte,Daniel B. Mendonça,Ivete L. Luz,Francisco C.O. Abreu,Elza A.S. de Mello,Gustavo W.S. Farias,Teresinha J.C. Ferreira,Afonso F.B. Millington,Maria A. Costa,Carlos H.N. |
author_role |
author |
author2 |
Mendonça,Ivete L. Luz,Francisco C.O. Abreu,Elza A.S. de Mello,Gustavo W.S. Farias,Teresinha J.C. Ferreira,Afonso F.B. Millington,Maria A. Costa,Carlos H.N. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Liarte,Daniel B. Mendonça,Ivete L. Luz,Francisco C.O. Abreu,Elza A.S. de Mello,Gustavo W.S. Farias,Teresinha J.C. Ferreira,Afonso F.B. Millington,Maria A. Costa,Carlos H.N. |
dc.subject.por.fl_str_mv |
Kala-azar Visceral leishmaniasis Leishmania chagasi Dog QBC® |
topic |
Kala-azar Visceral leishmaniasis Leishmania chagasi Dog QBC® |
description |
"Quantitative Buffy Coat" (QBC®) is a direct and fast fluorescent method used for the identification of blood parasites. Since Leishmania chagasi circulates in blood, we decided to test it in American visceral leishmaniasis (AVL). Bone marrow (BM) and peripheral blood (PB) of 49 persons and PB of 31 dogs were analyzed. QBC® was positive in BM of 11/11 patients with AVL and in 1/6 patients with other diseases. Amastigotes were identified in PB of 18/22 patients with AVL and in none without AVL. The test was positive in 30 out of the 31 seropositive dogs and in 28/28 dogs with Leishmania identified in other tissues. QBC® is a promising method for diagnosis of human AVL, and possibly for the exam of PB of patients with AVL/AIDS, for the control of the cure and for the identification of asymptomatic carriers. Because it is fast and easy to collect and execute, QBC® should be evaluated for programs of reservoir control. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000600013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000600013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822001000600013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.34 n.6 2001 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122151554514944 |