Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients

Detalhes bibliográficos
Autor(a) principal: Pannuti,Carolina V.
Data de Publicação: 2004
Outros Autores: Jorge,Maria Lúcia S.G., Biasutti,Cláudia, Kallás,Esper G., Segurado,Aluisio A.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008
Resumo: To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests.
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spelling Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patientsHTLV-ICultureCell lineBrazilTo investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests.Sociedade Brasileira de Medicina Tropical - SBMT2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008Revista da Sociedade Brasileira de Medicina Tropical v.37 n.4 2004reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822004000400008info:eu-repo/semantics/openAccessPannuti,Carolina V.Jorge,Maria Lúcia S.G.Biasutti,CláudiaKallás,Esper G.Segurado,Aluisio A.C.eng2004-08-20T00:00:00Zoai:scielo:S0037-86822004000400008Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2004-08-20T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
title Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
spellingShingle Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
Pannuti,Carolina V.
HTLV-I
Culture
Cell line
Brazil
title_short Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
title_full Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
title_fullStr Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
title_full_unstemmed Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
title_sort Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
author Pannuti,Carolina V.
author_facet Pannuti,Carolina V.
Jorge,Maria Lúcia S.G.
Biasutti,Cláudia
Kallás,Esper G.
Segurado,Aluisio A.C.
author_role author
author2 Jorge,Maria Lúcia S.G.
Biasutti,Cláudia
Kallás,Esper G.
Segurado,Aluisio A.C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pannuti,Carolina V.
Jorge,Maria Lúcia S.G.
Biasutti,Cláudia
Kallás,Esper G.
Segurado,Aluisio A.C.
dc.subject.por.fl_str_mv HTLV-I
Culture
Cell line
Brazil
topic HTLV-I
Culture
Cell line
Brazil
description To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests.
publishDate 2004
dc.date.none.fl_str_mv 2004-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86822004000400008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.37 n.4 2004
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
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