Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008 |
Resumo: | To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests. |
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Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patientsHTLV-ICultureCell lineBrazilTo investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests.Sociedade Brasileira de Medicina Tropical - SBMT2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008Revista da Sociedade Brasileira de Medicina Tropical v.37 n.4 2004reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822004000400008info:eu-repo/semantics/openAccessPannuti,Carolina V.Jorge,Maria Lúcia S.G.Biasutti,CláudiaKallás,Esper G.Segurado,Aluisio A.C.eng2004-08-20T00:00:00Zoai:scielo:S0037-86822004000400008Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2004-08-20T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
title |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
spellingShingle |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients Pannuti,Carolina V. HTLV-I Culture Cell line Brazil |
title_short |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
title_full |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
title_fullStr |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
title_full_unstemmed |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
title_sort |
Establishment of HTLV-I-infected cell lines from peripheral blood mononuclear cells of Brazilian patients |
author |
Pannuti,Carolina V. |
author_facet |
Pannuti,Carolina V. Jorge,Maria Lúcia S.G. Biasutti,Cláudia Kallás,Esper G. Segurado,Aluisio A.C. |
author_role |
author |
author2 |
Jorge,Maria Lúcia S.G. Biasutti,Cláudia Kallás,Esper G. Segurado,Aluisio A.C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pannuti,Carolina V. Jorge,Maria Lúcia S.G. Biasutti,Cláudia Kallás,Esper G. Segurado,Aluisio A.C. |
dc.subject.por.fl_str_mv |
HTLV-I Culture Cell line Brazil |
topic |
HTLV-I Culture Cell line Brazil |
description |
To investigate epidemiological and pathogenetic features of HTLV-I infection, a cohort of carriers has been followed at the USP Teaching Hospital since 1991. This study describes the establishment of cell lines from peripheral blood mononuclear cells (PBMC) of infected subjects. Ex vivo PBMC were cultured with those from a seronegative donor and morphologic evidence of cell transformation was obtained after 90 days with detection of multinucleated cells exhibiting cerebriform nuclei. Integration of HTLV-I proviral DNA and expression of viral antigens was demonstrated in culture by PCR and immunofluorescence. Cell lines were maintained for 240 days, gradually weaned from exogenous IL-2. Immunophenotyping of cell lines on flow cytometry yielded evidence of cell activation. Establishment of HTLV-I-infected cell lines from ex vivo PBMC is feasible and may be useful for studies on lymphocyte phenotypic changes and on mechanisms of HTLV-induced cell proliferation. Moreover they may be used with diagnostic purposes in immunofluorescence tests. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000400008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822004000400008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.37 n.4 2004 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
_version_ |
1752122152802320384 |