Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals

Bibliographic Details
Main Author: Carvalho Filho, Edgar Marcelino de
Publication Date: 2011
Other Authors: Porto, Maria Aurélia da Fonseca, Jesus, Amélia Maria Ribeiro de, Luna, Tania, Santos, S. B., Nascimento, M., Muniz, André Luís Nunes
Format: Article
Language: eng
Source: Repositório Institucional da UFBA
Download full: http://www.repositorio.ufba.br/ri/handle/ri/5425
Summary: p. 1134-1140
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spelling Carvalho Filho, Edgar Marcelino dePorto, Maria Aurélia da FonsecaJesus, Amélia Maria Ribeiro deLuna, TaniaSantos, S. B.Nascimento, M.Muniz, André Luís NunesCarvalho Filho, Edgar Marcelino dePorto, Maria Aurélia da FonsecaJesus, Amélia Maria Ribeiro deLuna, TaniaSantos, S. B.Nascimento, M.Muniz, André Luís Nunes2012-02-23T18:01:58Z2012-02-23T18:01:58Z20110034-7310http://www.repositorio.ufba.br/ri/handle/ri/5425v. 44.p. 1134-1140Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of pro-inflammatory cytokines by T cells, and increasing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels are potentially involved in tissue damage in diseases related to HTLV-1. This exaggerated immune response is also due to an inability of the natural regulatory mechanisms to down-modulate the immune response in this group of patients. TNF-α inhibitors reduce inflammation and have been shown to improve chronic inflammatory diseases in clinical trials. The aim of this study was to evaluate the ability of pentoxifylline, forskolin, rolipram, and thalidomide to decrease in vitro production of TNF-α and IFN-γ in cells of HTLV-1-infected subjects. Participants of the study included 19 patients with HAM/TSP (mean age, 53 ± 11; male:female ratio, 1:1) and 18 HTLV-1 carriers (mean age, 47 ± 11; male:female ratio, 1:2.6). Cytokines were determined by ELISA in supernatants of mononuclear cell cultures. Pentoxifylline inhibited TNF-α and IFN-γ synthesis with the minimum dose used (50 μM). The results with forskolin were similar to those observed with pentoxifylline. The doses of rolipram used were 0.01-1 μM and the best inhibition of TNF-α production was achieved with 1 μM and for IFN-γ production it was 0.01 μM. The minimum dose of thalidomide used (1 μM) inhibited TNF-α production but thalidomide did not inhibit IFN-γ production even when the maximum dose (50 μM) was used. All drugs had an in vitro inhibitory effect on TNF-α production and, with the exception of thalidomide, all of them also decreased IFN-γ production.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-23T18:01:58Z No. of bitstreams: 1 Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals.pdf: 671396 bytes, checksum: 2ec0eb464a9aec702483c68c08e84917 (MD5)Made available in DSpace on 2012-02-23T18:01:58Z (GMT). No. of bitstreams: 1 Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals.pdf: 671396 bytes, checksum: 2ec0eb464a9aec702483c68c08e84917 (MD5) Previous issue date: 2011doi: 10.1590/S0100-879X2011007500140reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAHTLV-1TNF-α inhibitorsPentoxifyllineImmune responseEffect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individualsBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALEffect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals.pdfEffect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals.pdfapplication/pdf671396https://repositorio.ufba.br/bitstream/ri/5425/1/Effect%20of%20TNF-%ce%b1%20production%20inhibitors%20on%20the%20production%20of%20pro-inflammatory%20cytokines%20by%20peripheral%20blood%20mononuclear%20cells%20from%20HTLV-1-infected%20individuals.pdf2ec0eb464a9aec702483c68c08e84917MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5425/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTEffect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals.pdf.txtEffect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals.pdf.txtExtracted texttext/plain32381https://repositorio.ufba.br/bitstream/ri/5425/3/Effect%20of%20TNF-%ce%b1%20production%20inhibitors%20on%20the%20production%20of%20pro-inflammatory%20cytokines%20by%20peripheral%20blood%20mononuclear%20cells%20from%20HTLV-1-infected%20individuals.pdf.txt759eac05ccf7a7f2e4c5bf5036487a1aMD53ri/54252022-07-05 14:03:13.613oai:repositorio.ufba.br:ri/5425VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:13Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
dc.title.alternative.pt_BR.fl_str_mv Brazilian Journal of Medical and Biological Research
title Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
spellingShingle Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
Carvalho Filho, Edgar Marcelino de
HTLV-1
TNF-α inhibitors
Pentoxifylline
Immune response
title_short Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
title_full Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
title_fullStr Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
title_full_unstemmed Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
title_sort Effect of TNF-alfa production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1 infected individuals
author Carvalho Filho, Edgar Marcelino de
author_facet Carvalho Filho, Edgar Marcelino de
Porto, Maria Aurélia da Fonseca
Jesus, Amélia Maria Ribeiro de
Luna, Tania
Santos, S. B.
Nascimento, M.
Muniz, André Luís Nunes
author_role author
author2 Porto, Maria Aurélia da Fonseca
Jesus, Amélia Maria Ribeiro de
Luna, Tania
Santos, S. B.
Nascimento, M.
Muniz, André Luís Nunes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho Filho, Edgar Marcelino de
Porto, Maria Aurélia da Fonseca
Jesus, Amélia Maria Ribeiro de
Luna, Tania
Santos, S. B.
Nascimento, M.
Muniz, André Luís Nunes
Carvalho Filho, Edgar Marcelino de
Porto, Maria Aurélia da Fonseca
Jesus, Amélia Maria Ribeiro de
Luna, Tania
Santos, S. B.
Nascimento, M.
Muniz, André Luís Nunes
dc.subject.por.fl_str_mv HTLV-1
TNF-α inhibitors
Pentoxifylline
Immune response
topic HTLV-1
TNF-α inhibitors
Pentoxifylline
Immune response
description p. 1134-1140
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-23T18:01:58Z
dc.date.available.fl_str_mv 2012-02-23T18:01:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5425
dc.identifier.issn.none.fl_str_mv 0034-7310
dc.identifier.number.pt_BR.fl_str_mv v. 44.
identifier_str_mv 0034-7310
v. 44.
url http://www.repositorio.ufba.br/ri/handle/ri/5425
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv doi: 10.1590/S0100-879X2011007500140
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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