Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen

Detalhes bibliográficos
Autor(a) principal: Vitral,Claudia Lamarca
Data de Publicação: 1991
Outros Autores: Gaspar,Ana Maria Coimbra, Yoshida,Clara Fumiko Tachibana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003
Resumo: Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.
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spelling Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigenEnzyme immunoassayAnti-HAVHepatitis ATwo competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.Sociedade Brasileira de Medicina Tropical - SBMT1991-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003Revista da Sociedade Brasileira de Medicina Tropical v.24 n.2 1991reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86821991000200003info:eu-repo/semantics/openAccessVitral,Claudia LamarcaGaspar,Ana Maria CoimbraYoshida,Clara Fumiko Tachibanaeng2013-05-15T00:00:00Zoai:scielo:S0037-86821991000200003Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2013-05-15T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
title Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
spellingShingle Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
Vitral,Claudia Lamarca
Enzyme immunoassay
Anti-HAV
Hepatitis A
title_short Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
title_full Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
title_fullStr Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
title_full_unstemmed Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
title_sort Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
author Vitral,Claudia Lamarca
author_facet Vitral,Claudia Lamarca
Gaspar,Ana Maria Coimbra
Yoshida,Clara Fumiko Tachibana
author_role author
author2 Gaspar,Ana Maria Coimbra
Yoshida,Clara Fumiko Tachibana
author2_role author
author
dc.contributor.author.fl_str_mv Vitral,Claudia Lamarca
Gaspar,Ana Maria Coimbra
Yoshida,Clara Fumiko Tachibana
dc.subject.por.fl_str_mv Enzyme immunoassay
Anti-HAV
Hepatitis A
topic Enzyme immunoassay
Anti-HAV
Hepatitis A
description Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; in the second (COMP-2), test sera and anti-HA VHRP conjugate competed for HAV epitopes previously adsorbed to anti-HA V IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. Both competitive tests were shown to be reproducible and suitable for routine diagnosis and research purposes.
publishDate 1991
dc.date.none.fl_str_mv 1991-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821991000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86821991000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.24 n.2 1991
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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