Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae

Detalhes bibliográficos
Autor(a) principal: Guerrero,Jorge
Data de Publicação: 1973
Outros Autores: Silverman,Paul H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821973000500005
Resumo: Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.
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spelling Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvaeAscaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.Sociedade Brasileira de Medicina Tropical - SBMT1973-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821973000500005Revista da Sociedade Brasileira de Medicina Tropical v.7 n.5 1973reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86821973000500005info:eu-repo/semantics/openAccessGuerrero,JorgeSilverman,Paul H.eng2013-06-21T00:00:00Zoai:scielo:S0037-86821973000500005Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2013-06-21T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
title Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
spellingShingle Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
Guerrero,Jorge
title_short Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
title_full Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
title_fullStr Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
title_full_unstemmed Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
title_sort Ascaris suum: partial fractionation of metabolic antigens from "in vitro" cultured larvae
author Guerrero,Jorge
author_facet Guerrero,Jorge
Silverman,Paul H.
author_role author
author2 Silverman,Paul H.
author2_role author
dc.contributor.author.fl_str_mv Guerrero,Jorge
Silverman,Paul H.
description Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.
publishDate 1973
dc.date.none.fl_str_mv 1973-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821973000500005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821973000500005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86821973000500005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.7 n.5 1973
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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