Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae

Detalhes bibliográficos
Autor(a) principal: Guerrero, Jorge [UNIFESP]
Data de Publicação: 1973
Outros Autores: Silverman, Paul H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0037-86821973000500005
http://repositorio.unifesp.br/handle/11600/154
Resumo: Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.
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spelling Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvaeAscaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.Os antígenos metabólicos de Ascaris suum foram obtidos de larvas de segundo e de terceiro estágio (precoce) cultivadas in vitro em meio mínimo essencial de Eeagle com suplemento proteico. Os antígenos metabólicos coletados depois de 12 a 16 dias das culturas in vitro foram eluidos em Bio-Gel Al 5. Três picos de eluição foram identificados, dializados, liofilizados e injetados em camundongos, com alginato de sódio a 4%. O pico II da eluição de duas preparações dos antígenos metabólicos protegeram os camundongos da infecção com 10.000 ovos infectivos de A. suumEscola Paulista de Medicina Centro de Pesquisas em Fisiologia e Imunologia de Helmintos Departamento de Microbiologia e ParasitologiaUniversity of New Mexico Department of BiologyUNIFESP, EPM, Centro de Pesquisas em Fisiologia e Imunologia de Helmintos Depto. de Microbiologia e ParasitologiaSciELOSociedade Brasileira de Medicina Tropical - SBMTUniversidade Federal de São Paulo (UNIFESP)University of New Mexico Department of BiologyGuerrero, Jorge [UNIFESP]Silverman, Paul H.2015-06-14T13:24:12Z2015-06-14T13:24:12Z1973-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion319-327application/pdfhttp://dx.doi.org/10.1590/S0037-86821973000500005Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 7, n. 5, p. 319-327, 1973.10.1590/S0037-86821973000500005S0037-86821973000500005.pdf0037-8682S0037-86821973000500005http://repositorio.unifesp.br/handle/11600/154engRevista da Sociedade Brasileira de Medicina Tropicalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T03:51:59Zoai:repositorio.unifesp.br/:11600/154Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T03:51:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
title Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
spellingShingle Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
Guerrero, Jorge [UNIFESP]
title_short Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
title_full Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
title_fullStr Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
title_full_unstemmed Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
title_sort Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae
author Guerrero, Jorge [UNIFESP]
author_facet Guerrero, Jorge [UNIFESP]
Silverman, Paul H.
author_role author
author2 Silverman, Paul H.
author2_role author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
University of New Mexico Department of Biology
dc.contributor.author.fl_str_mv Guerrero, Jorge [UNIFESP]
Silverman, Paul H.
description Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.
publishDate 1973
dc.date.none.fl_str_mv 1973-10-01
2015-06-14T13:24:12Z
2015-06-14T13:24:12Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0037-86821973000500005
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 7, n. 5, p. 319-327, 1973.
10.1590/S0037-86821973000500005
S0037-86821973000500005.pdf
0037-8682
S0037-86821973000500005
http://repositorio.unifesp.br/handle/11600/154
url http://dx.doi.org/10.1590/S0037-86821973000500005
http://repositorio.unifesp.br/handle/11600/154
identifier_str_mv Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 7, n. 5, p. 319-327, 1973.
10.1590/S0037-86821973000500005
S0037-86821973000500005.pdf
0037-8682
S0037-86821973000500005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 319-327
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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