Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034 |
Resumo: | INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil. |
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Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern BrazilStaphylococcus aureusMethicillin-resistantSkin infectionsSoft tissue infections INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.Sociedade Brasileira de Medicina Tropical - SBMT2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-868213022013info:eu-repo/semantics/openAccessGelatti,Luciane CristinaBonamigo,Renan RangelInoue,Fernanda MatsikoCarmo,Mirian Silva doBecker,Ana PaulaCastrucci,Fernanda Marques da SilvaPignatari,Antônio Carlos Camposd' Azevedo,Pedro Alveseng2021-03-18T00:00:00Zoai:scielo:S0037-86822013000100034Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2021-03-18T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
title |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
spellingShingle |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil Gelatti,Luciane Cristina Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections |
title_short |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
title_full |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
title_fullStr |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
title_full_unstemmed |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
title_sort |
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil |
author |
Gelatti,Luciane Cristina |
author_facet |
Gelatti,Luciane Cristina Bonamigo,Renan Rangel Inoue,Fernanda Matsiko Carmo,Mirian Silva do Becker,Ana Paula Castrucci,Fernanda Marques da Silva Pignatari,Antônio Carlos Campos d' Azevedo,Pedro Alves |
author_role |
author |
author2 |
Bonamigo,Renan Rangel Inoue,Fernanda Matsiko Carmo,Mirian Silva do Becker,Ana Paula Castrucci,Fernanda Marques da Silva Pignatari,Antônio Carlos Campos d' Azevedo,Pedro Alves |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Gelatti,Luciane Cristina Bonamigo,Renan Rangel Inoue,Fernanda Matsiko Carmo,Mirian Silva do Becker,Ana Paula Castrucci,Fernanda Marques da Silva Pignatari,Antônio Carlos Campos d' Azevedo,Pedro Alves |
dc.subject.por.fl_str_mv |
Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections |
topic |
Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections |
description |
INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0037-868213022013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122158257012736 |