Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil

Detalhes bibliográficos
Autor(a) principal: Gelatti,Luciane Cristina
Data de Publicação: 2013
Outros Autores: Bonamigo,Renan Rangel, Inoue,Fernanda Matsiko, Carmo,Mirian Silva do, Becker,Ana Paula, Castrucci,Fernanda Marques da Silva, Pignatari,Antônio Carlos Campos, d' Azevedo,Pedro Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034
Resumo: INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.
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spelling Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern BrazilStaphylococcus aureusMethicillin-resistantSkin infectionsSoft tissue infections INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.Sociedade Brasileira de Medicina Tropical - SBMT2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-868213022013info:eu-repo/semantics/openAccessGelatti,Luciane CristinaBonamigo,Renan RangelInoue,Fernanda MatsikoCarmo,Mirian Silva doBecker,Ana PaulaCastrucci,Fernanda Marques da SilvaPignatari,Antônio Carlos Camposd' Azevedo,Pedro Alveseng2021-03-18T00:00:00Zoai:scielo:S0037-86822013000100034Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2021-03-18T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
title Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
spellingShingle Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
Gelatti,Luciane Cristina
Staphylococcus aureus
Methicillin-resistant
Skin infections
Soft tissue infections
title_short Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
title_full Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
title_fullStr Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
title_full_unstemmed Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
title_sort Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil
author Gelatti,Luciane Cristina
author_facet Gelatti,Luciane Cristina
Bonamigo,Renan Rangel
Inoue,Fernanda Matsiko
Carmo,Mirian Silva do
Becker,Ana Paula
Castrucci,Fernanda Marques da Silva
Pignatari,Antônio Carlos Campos
d' Azevedo,Pedro Alves
author_role author
author2 Bonamigo,Renan Rangel
Inoue,Fernanda Matsiko
Carmo,Mirian Silva do
Becker,Ana Paula
Castrucci,Fernanda Marques da Silva
Pignatari,Antônio Carlos Campos
d' Azevedo,Pedro Alves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gelatti,Luciane Cristina
Bonamigo,Renan Rangel
Inoue,Fernanda Matsiko
Carmo,Mirian Silva do
Becker,Ana Paula
Castrucci,Fernanda Marques da Silva
Pignatari,Antônio Carlos Campos
d' Azevedo,Pedro Alves
dc.subject.por.fl_str_mv Staphylococcus aureus
Methicillin-resistant
Skin infections
Soft tissue infections
topic Staphylococcus aureus
Methicillin-resistant
Skin infections
Soft tissue infections
description INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-01
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-868213022013
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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