Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders

Detalhes bibliográficos
Autor(a) principal: Langoni,Helio
Data de Publicação: 2012
Outros Autores: Matteucci,Guilherme, Medici,Bruno, Camossi,Lucilene Granuzio, Richini-Pereira,Virgínia Bodelão, Silva,Rodrigo Costa da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000300016
Resumo: INTRODUCTION: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. METHODS: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5′-3′-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. RESULTS: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95%I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. CONCLUSIONS: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.
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spelling Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disordersToxoplasma gondiiNeospora caninumGenotypesDogsRFLP-PCRZoonosisINTRODUCTION: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. METHODS: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5′-3′-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. RESULTS: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95%I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. CONCLUSIONS: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.Sociedade Brasileira de Medicina Tropical - SBMT2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000300016Revista da Sociedade Brasileira de Medicina Tropical v.45 n.3 2012reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822012000300016info:eu-repo/semantics/openAccessLangoni,HelioMatteucci,GuilhermeMedici,BrunoCamossi,Lucilene GranuzioRichini-Pereira,Virgínia BodelãoSilva,Rodrigo Costa daeng2021-04-07T00:00:00Zoai:scielo:S0037-86822012000300016Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2021-04-07T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
spellingShingle Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
Langoni,Helio
Toxoplasma gondii
Neospora caninum
Genotypes
Dogs
RFLP-PCR
Zoonosis
title_short Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_full Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_fullStr Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_full_unstemmed Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_sort Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
author Langoni,Helio
author_facet Langoni,Helio
Matteucci,Guilherme
Medici,Bruno
Camossi,Lucilene Granuzio
Richini-Pereira,Virgínia Bodelão
Silva,Rodrigo Costa da
author_role author
author2 Matteucci,Guilherme
Medici,Bruno
Camossi,Lucilene Granuzio
Richini-Pereira,Virgínia Bodelão
Silva,Rodrigo Costa da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Langoni,Helio
Matteucci,Guilherme
Medici,Bruno
Camossi,Lucilene Granuzio
Richini-Pereira,Virgínia Bodelão
Silva,Rodrigo Costa da
dc.subject.por.fl_str_mv Toxoplasma gondii
Neospora caninum
Genotypes
Dogs
RFLP-PCR
Zoonosis
topic Toxoplasma gondii
Neospora caninum
Genotypes
Dogs
RFLP-PCR
Zoonosis
description INTRODUCTION: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. METHODS: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5′-3′-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. RESULTS: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95%I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. CONCLUSIONS: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/S0037-86822012000300016
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.45 n.3 2012
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
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