Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders

Detalhes bibliográficos
Autor(a) principal: Langoni, Hélio [UNESP]
Data de Publicação: 2012
Outros Autores: Matteucci, Guilherme [UNESP], Medici, Bruno [UNESP], Camossi, Lucilene Granuzio [UNESP], Richini-Pereira, Virginia Bodelao [UNESP], da Silva, Rodrigo Costa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0037-86822012000300016
http://hdl.handle.net/11449/13870
Resumo: Introduction: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. Methods: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5'-3'-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. Results: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95% I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. Conclusions: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.
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spelling Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disordersDetecção e análise molecular de Toxoplasma gondii e Neospora caninum em cães com distúrbios neurológicosToxoplasma gondiiNeospora caninumGenotypesDogsRFLP-PCRZoonosisIntroduction: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. Methods: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5'-3'-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. Results: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95% I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. Conclusions: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.INTRODUÇÃO: Toxoplasma gondii e Neospora caninum são parasitas Apicomplexa responsáveis por doenças sistêmicas em muitas espécies de animais, incluindo cães, o que representa grande importância em animais de estimação. MÉTODOS: Este estudo teve como objetivo determinar a prevalência da infecção de T. gondii e N. caninum em 50 cães com sinais neurológicos internados no Hospital Veterinário da Universidade Estadual Paulista (UNESP) na Cidade de Botucatu, Brasil. Todos os animais foram examinados para detecção de anticorpos por IFAT para ambos os parasitas. Tecidos de animais positivos foram analisados por bioensaio em camundongos (T. gondii) e gerbilos (N. caninum) e o DNA foi pesquisado por PCR. Amostras positivas para T. gondii por PCR foram analisadas por meio de análise de restrição de fragmentos polimórficos (restriction fragment length polymorphism-polymerase chain reaction - RFLP-PCR), utilizando-se 11 marcadores: SAG1, SAG2 (5'-3'SAG2 e, alt.SAG2), SAG3, Btub, GRA6, L358, c22 -8, C29-6, PK1 e Apico e o marcador CS3 para análise de virulência. RESULTADOS: Os anticorpos específicos foram detectados em 11/50 animais (22%; IC95% 12,8-35,3%) para T. gondii, e 7/50 (14%; IC95% 7,0-26,3%) para N. caninum. No bioensaio e PCR, 7/11 (63,6%; IC95% 34,9-84,8%) das amostras foram positivas para T. gondii, e 3/7 (42,9%; IC95% 15,7-75,5%) para N. caninum. Três diferentes genótipos foram identificados. Apenas um foi único. CONCLUSÕES: Estes dados confirmam a presença de T. gondii e N. caninum em cães do Brasil, e demonstra a importância do T. gondii como sentinela para a infecção e a variação genotípica deste parasita no Brasil.Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saúde Publica, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saúde Publica, Botucatu, SP, BrazilSoc Brasileira Medicina TropicalUniversidade Estadual Paulista (Unesp)Langoni, Hélio [UNESP]Matteucci, Guilherme [UNESP]Medici, Bruno [UNESP]Camossi, Lucilene Granuzio [UNESP]Richini-Pereira, Virginia Bodelao [UNESP]da Silva, Rodrigo Costa [UNESP]2014-05-20T13:39:57Z2014-05-20T13:39:57Z2012-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article365-368application/pdfhttp://dx.doi.org/10.1590/S0037-86822012000300016Revista da Sociedade Brasileira de Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 45, n. 3, p. 365-368, 2012.0037-8682http://hdl.handle.net/11449/13870S0037-86822012000300016WOS:000305980500016S0037-86822012000300016-en.pdf5326072118518067Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropical1.3580,658info:eu-repo/semantics/openAccess2023-11-09T06:08:39Zoai:repositorio.unesp.br:11449/13870Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:12:45.062979Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
Detecção e análise molecular de Toxoplasma gondii e Neospora caninum em cães com distúrbios neurológicos
title Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
spellingShingle Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
Langoni, Hélio [UNESP]
Toxoplasma gondii
Neospora caninum
Genotypes
Dogs
RFLP-PCR
Zoonosis
title_short Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_full Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_fullStr Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_full_unstemmed Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
title_sort Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
author Langoni, Hélio [UNESP]
author_facet Langoni, Hélio [UNESP]
Matteucci, Guilherme [UNESP]
Medici, Bruno [UNESP]
Camossi, Lucilene Granuzio [UNESP]
Richini-Pereira, Virginia Bodelao [UNESP]
da Silva, Rodrigo Costa [UNESP]
author_role author
author2 Matteucci, Guilherme [UNESP]
Medici, Bruno [UNESP]
Camossi, Lucilene Granuzio [UNESP]
Richini-Pereira, Virginia Bodelao [UNESP]
da Silva, Rodrigo Costa [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Langoni, Hélio [UNESP]
Matteucci, Guilherme [UNESP]
Medici, Bruno [UNESP]
Camossi, Lucilene Granuzio [UNESP]
Richini-Pereira, Virginia Bodelao [UNESP]
da Silva, Rodrigo Costa [UNESP]
dc.subject.por.fl_str_mv Toxoplasma gondii
Neospora caninum
Genotypes
Dogs
RFLP-PCR
Zoonosis
topic Toxoplasma gondii
Neospora caninum
Genotypes
Dogs
RFLP-PCR
Zoonosis
description Introduction: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. Methods: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5'-3'-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. Results: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95% I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. Conclusions: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-01
2014-05-20T13:39:57Z
2014-05-20T13:39:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0037-86822012000300016
Revista da Sociedade Brasileira de Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 45, n. 3, p. 365-368, 2012.
0037-8682
http://hdl.handle.net/11449/13870
S0037-86822012000300016
WOS:000305980500016
S0037-86822012000300016-en.pdf
5326072118518067
url http://dx.doi.org/10.1590/S0037-86822012000300016
http://hdl.handle.net/11449/13870
identifier_str_mv Revista da Sociedade Brasileira de Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 45, n. 3, p. 365-368, 2012.
0037-8682
S0037-86822012000300016
WOS:000305980500016
S0037-86822012000300016-en.pdf
5326072118518067
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
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0,658
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dc.format.none.fl_str_mv 365-368
application/pdf
dc.publisher.none.fl_str_mv Soc Brasileira Medicina Tropical
publisher.none.fl_str_mv Soc Brasileira Medicina Tropical
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
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reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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