Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0037-86822012000300016 http://hdl.handle.net/11449/13870 |
Resumo: | Introduction: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. Methods: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5'-3'-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. Results: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95% I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. Conclusions: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil. |
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Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disordersDetecção e análise molecular de Toxoplasma gondii e Neospora caninum em cães com distúrbios neurológicosToxoplasma gondiiNeospora caninumGenotypesDogsRFLP-PCRZoonosisIntroduction: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. Methods: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5'-3'-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. Results: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95% I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. Conclusions: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.INTRODUÇÃO: Toxoplasma gondii e Neospora caninum são parasitas Apicomplexa responsáveis por doenças sistêmicas em muitas espécies de animais, incluindo cães, o que representa grande importância em animais de estimação. MÉTODOS: Este estudo teve como objetivo determinar a prevalência da infecção de T. gondii e N. caninum em 50 cães com sinais neurológicos internados no Hospital Veterinário da Universidade Estadual Paulista (UNESP) na Cidade de Botucatu, Brasil. Todos os animais foram examinados para detecção de anticorpos por IFAT para ambos os parasitas. Tecidos de animais positivos foram analisados por bioensaio em camundongos (T. gondii) e gerbilos (N. caninum) e o DNA foi pesquisado por PCR. Amostras positivas para T. gondii por PCR foram analisadas por meio de análise de restrição de fragmentos polimórficos (restriction fragment length polymorphism-polymerase chain reaction - RFLP-PCR), utilizando-se 11 marcadores: SAG1, SAG2 (5'-3'SAG2 e, alt.SAG2), SAG3, Btub, GRA6, L358, c22 -8, C29-6, PK1 e Apico e o marcador CS3 para análise de virulência. RESULTADOS: Os anticorpos específicos foram detectados em 11/50 animais (22%; IC95% 12,8-35,3%) para T. gondii, e 7/50 (14%; IC95% 7,0-26,3%) para N. caninum. No bioensaio e PCR, 7/11 (63,6%; IC95% 34,9-84,8%) das amostras foram positivas para T. gondii, e 3/7 (42,9%; IC95% 15,7-75,5%) para N. caninum. Três diferentes genótipos foram identificados. Apenas um foi único. CONCLUSÕES: Estes dados confirmam a presença de T. gondii e N. caninum em cães do Brasil, e demonstra a importância do T. gondii como sentinela para a infecção e a variação genotípica deste parasita no Brasil.Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saúde Publica, Botucatu, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saúde Publica, Botucatu, SP, BrazilSoc Brasileira Medicina TropicalUniversidade Estadual Paulista (Unesp)Langoni, Hélio [UNESP]Matteucci, Guilherme [UNESP]Medici, Bruno [UNESP]Camossi, Lucilene Granuzio [UNESP]Richini-Pereira, Virginia Bodelao [UNESP]da Silva, Rodrigo Costa [UNESP]2014-05-20T13:39:57Z2014-05-20T13:39:57Z2012-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article365-368application/pdfhttp://dx.doi.org/10.1590/S0037-86822012000300016Revista da Sociedade Brasileira de Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 45, n. 3, p. 365-368, 2012.0037-8682http://hdl.handle.net/11449/13870S0037-86822012000300016WOS:000305980500016S0037-86822012000300016-en.pdf5326072118518067Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropical1.3580,658info:eu-repo/semantics/openAccess2023-11-09T06:08:39Zoai:repositorio.unesp.br:11449/13870Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:12:45.062979Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders Detecção e análise molecular de Toxoplasma gondii e Neospora caninum em cães com distúrbios neurológicos |
title |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders |
spellingShingle |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders Langoni, Hélio [UNESP] Toxoplasma gondii Neospora caninum Genotypes Dogs RFLP-PCR Zoonosis |
title_short |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders |
title_full |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders |
title_fullStr |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders |
title_full_unstemmed |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders |
title_sort |
Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders |
author |
Langoni, Hélio [UNESP] |
author_facet |
Langoni, Hélio [UNESP] Matteucci, Guilherme [UNESP] Medici, Bruno [UNESP] Camossi, Lucilene Granuzio [UNESP] Richini-Pereira, Virginia Bodelao [UNESP] da Silva, Rodrigo Costa [UNESP] |
author_role |
author |
author2 |
Matteucci, Guilherme [UNESP] Medici, Bruno [UNESP] Camossi, Lucilene Granuzio [UNESP] Richini-Pereira, Virginia Bodelao [UNESP] da Silva, Rodrigo Costa [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Langoni, Hélio [UNESP] Matteucci, Guilherme [UNESP] Medici, Bruno [UNESP] Camossi, Lucilene Granuzio [UNESP] Richini-Pereira, Virginia Bodelao [UNESP] da Silva, Rodrigo Costa [UNESP] |
dc.subject.por.fl_str_mv |
Toxoplasma gondii Neospora caninum Genotypes Dogs RFLP-PCR Zoonosis |
topic |
Toxoplasma gondii Neospora caninum Genotypes Dogs RFLP-PCR Zoonosis |
description |
Introduction: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. Methods: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5'-3'-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. Results: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95% I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. Conclusions: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-01 2014-05-20T13:39:57Z 2014-05-20T13:39:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0037-86822012000300016 Revista da Sociedade Brasileira de Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 45, n. 3, p. 365-368, 2012. 0037-8682 http://hdl.handle.net/11449/13870 S0037-86822012000300016 WOS:000305980500016 S0037-86822012000300016-en.pdf 5326072118518067 |
url |
http://dx.doi.org/10.1590/S0037-86822012000300016 http://hdl.handle.net/11449/13870 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Brasilia: Soc Brasileira Medicina Tropical, v. 45, n. 3, p. 365-368, 2012. 0037-8682 S0037-86822012000300016 WOS:000305980500016 S0037-86822012000300016-en.pdf 5326072118518067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical 1.358 0,658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
365-368 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Medicina Tropical |
publisher.none.fl_str_mv |
Soc Brasileira Medicina Tropical |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128773255593984 |