Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods

• Autor(a) principal: Mota,Crystiane Rodrigues Araújo
• Data de Publicação: 2009
• Outros Autores: Miranda,Karla Carvalho, Lemos,Janine de Aquino, Costa,Carolina Rodrigues, Souza,Lúcia Kioko Hasimoto e, Passos,Xisto Sena, Silva,Hildene Meneses e, Silva,Maria do Rosário Rodrigues
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822009000300003
• topic: In vitro susceptibility; Agar dilution; Broth microdilution; Dermatophytes

The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.