Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting

Detalhes bibliográficos
Autor(a) principal: Peixoto,Aline dos Santos
Data de Publicação: 2020
Outros Autores: Montenegro,Lílian Maria Lapa, Lima,Andrea Santos, Melo,Fábio Lopes, Barbosa Júnior,Walter Lins, Neves,Maria Madileuza Carneiro, Ramos,Jesus Pais, Schindler,Haiana Charifker, Medeiros,Zulma Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100372
Resumo: Abstract INTRODUCTION: Nontuberculous mycobacteria (NTM) species, as human pathogens, are increasing in the world, as is the difficulty of accurately identifying them. Differential diagnosis, especially between the M. tuberculosis complex and NTM species, and the characterization of NTM species is important. This study aimed to evaluate the performance of a molecular system based on multiplex real-time PCR with high-resolution melting (HRM) for the identification and differentiation of NTM species of clinical importance of an endemic area for tuberculosis in northeastern Brazil. METHODS: The technical protocol of the molecular system was based on multiplex real-time PCR-HRM, and evaluated the sensitivity and specificity of the detection of NTM species in mycobacterial clinical isolates from the studied region. The gold standard method was specific gene sequencing. RESULTS: The sensitivity and specificity of multiplex real-time PCR-HRM modified for differentiation between NTM and M. tuberculosis were 90% and 100%, respectively. The PCR-HRM sensitivities for the characterization of NTM species (M. kansasii, M. abscesses, M. avium, and M. fortuitum) were 94.59%, 80%, 57.14%, and 54%, respectively. CONCLUSIONS The multiplex real-time PCR-HRM modified assay has the potential to rapidly and efficiently identify nontuberculous mycobacteria of clinical importance, which is crucial for immediate implementation of the appropriate therapy and thus avoiding complications and sequelae in patients.
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spelling Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution meltingIdentificationNon-tuberculous mycobacteriaPCR multiplex real-timeDiagnosisBrazilAbstract INTRODUCTION: Nontuberculous mycobacteria (NTM) species, as human pathogens, are increasing in the world, as is the difficulty of accurately identifying them. Differential diagnosis, especially between the M. tuberculosis complex and NTM species, and the characterization of NTM species is important. This study aimed to evaluate the performance of a molecular system based on multiplex real-time PCR with high-resolution melting (HRM) for the identification and differentiation of NTM species of clinical importance of an endemic area for tuberculosis in northeastern Brazil. METHODS: The technical protocol of the molecular system was based on multiplex real-time PCR-HRM, and evaluated the sensitivity and specificity of the detection of NTM species in mycobacterial clinical isolates from the studied region. The gold standard method was specific gene sequencing. RESULTS: The sensitivity and specificity of multiplex real-time PCR-HRM modified for differentiation between NTM and M. tuberculosis were 90% and 100%, respectively. The PCR-HRM sensitivities for the characterization of NTM species (M. kansasii, M. abscesses, M. avium, and M. fortuitum) were 94.59%, 80%, 57.14%, and 54%, respectively. CONCLUSIONS The multiplex real-time PCR-HRM modified assay has the potential to rapidly and efficiently identify nontuberculous mycobacteria of clinical importance, which is crucial for immediate implementation of the appropriate therapy and thus avoiding complications and sequelae in patients.Sociedade Brasileira de Medicina Tropical - SBMT2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100372Revista da Sociedade Brasileira de Medicina Tropical v.53 2020reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0211-2020info:eu-repo/semantics/openAccessPeixoto,Aline dos SantosMontenegro,Lílian Maria LapaLima,Andrea SantosMelo,Fábio LopesBarbosa Júnior,Walter LinsNeves,Maria Madileuza CarneiroRamos,Jesus PaisSchindler,Haiana CharifkerMedeiros,Zulma Mariaeng2020-12-11T00:00:00Zoai:scielo:S0037-86822020000100372Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2020-12-11T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
title Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
spellingShingle Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
Peixoto,Aline dos Santos
Identification
Non-tuberculous mycobacteria
PCR multiplex real-time
Diagnosis
Brazil
title_short Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
title_full Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
title_fullStr Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
title_full_unstemmed Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
title_sort Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
author Peixoto,Aline dos Santos
author_facet Peixoto,Aline dos Santos
Montenegro,Lílian Maria Lapa
Lima,Andrea Santos
Melo,Fábio Lopes
Barbosa Júnior,Walter Lins
Neves,Maria Madileuza Carneiro
Ramos,Jesus Pais
Schindler,Haiana Charifker
Medeiros,Zulma Maria
author_role author
author2 Montenegro,Lílian Maria Lapa
Lima,Andrea Santos
Melo,Fábio Lopes
Barbosa Júnior,Walter Lins
Neves,Maria Madileuza Carneiro
Ramos,Jesus Pais
Schindler,Haiana Charifker
Medeiros,Zulma Maria
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Peixoto,Aline dos Santos
Montenegro,Lílian Maria Lapa
Lima,Andrea Santos
Melo,Fábio Lopes
Barbosa Júnior,Walter Lins
Neves,Maria Madileuza Carneiro
Ramos,Jesus Pais
Schindler,Haiana Charifker
Medeiros,Zulma Maria
dc.subject.por.fl_str_mv Identification
Non-tuberculous mycobacteria
PCR multiplex real-time
Diagnosis
Brazil
topic Identification
Non-tuberculous mycobacteria
PCR multiplex real-time
Diagnosis
Brazil
description Abstract INTRODUCTION: Nontuberculous mycobacteria (NTM) species, as human pathogens, are increasing in the world, as is the difficulty of accurately identifying them. Differential diagnosis, especially between the M. tuberculosis complex and NTM species, and the characterization of NTM species is important. This study aimed to evaluate the performance of a molecular system based on multiplex real-time PCR with high-resolution melting (HRM) for the identification and differentiation of NTM species of clinical importance of an endemic area for tuberculosis in northeastern Brazil. METHODS: The technical protocol of the molecular system was based on multiplex real-time PCR-HRM, and evaluated the sensitivity and specificity of the detection of NTM species in mycobacterial clinical isolates from the studied region. The gold standard method was specific gene sequencing. RESULTS: The sensitivity and specificity of multiplex real-time PCR-HRM modified for differentiation between NTM and M. tuberculosis were 90% and 100%, respectively. The PCR-HRM sensitivities for the characterization of NTM species (M. kansasii, M. abscesses, M. avium, and M. fortuitum) were 94.59%, 80%, 57.14%, and 54%, respectively. CONCLUSIONS The multiplex real-time PCR-HRM modified assay has the potential to rapidly and efficiently identify nontuberculous mycobacteria of clinical importance, which is crucial for immediate implementation of the appropriate therapy and thus avoiding complications and sequelae in patients.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100372
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100372
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0211-2020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.53 2020
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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