Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100339 |
Resumo: | Abstract INTRODUCTION: Trypanosoma cruzi infection triggers an inflammatory process with exacerbated production of cytokines that stimulate inflammatory and anti-inflammatory signals, including the efferent anti-inflammatory signal known as the anti-inflammatory cholinergic pathway. Thus, the use of anticholinesterase drugs, such as galantamine, could minimize the inflammatory process caused by this disease. METHODS For the study at 30, 60, and 90 days, 120 Swiss mice were divided into three groups. Each group was subdivided into four subgroups: uninfected/untreated (CTRL), uninfected/treated (GAL), infected/untreated (INF), and infected/treated (GAL/INF). The infected groups were inoculated intraperitoneally with 0.1 ml of mouse blood containing 5 × 104 trypomastigote forms of the T. cruzi QM2 strain. The galantamine-treated groups received 5 mg/kg of galantamine orally, through pipetting. From each subgroup, the parameters of parasitemia, histopathological analysis, butyrylcholinesterase activity (BuChE), and functional study of the colon were evaluated. RESULTS: BuChE performance was observed when AChE was suppressed, with increased activity in the GAL/INF group similar to the INF group on the 30th day post infection, thus corroborating the absence of a significant difference in parasitic curves and histopathological analysis. CONCLUSIONS: The presence of an inflammatory process and nests of amastigotes, as well as evidence of reactivity to ACh and NOR, suggest that galantamine did not interfere with the colonic inflammatory response or even in colonic tissue parasitism at this stage of Chagas disease. |
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Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strainTrypanosoma cruziAcethylcholinesteraseButyrylcholinesteraseInflammatory processColonChagas diseaseAbstract INTRODUCTION: Trypanosoma cruzi infection triggers an inflammatory process with exacerbated production of cytokines that stimulate inflammatory and anti-inflammatory signals, including the efferent anti-inflammatory signal known as the anti-inflammatory cholinergic pathway. Thus, the use of anticholinesterase drugs, such as galantamine, could minimize the inflammatory process caused by this disease. METHODS For the study at 30, 60, and 90 days, 120 Swiss mice were divided into three groups. Each group was subdivided into four subgroups: uninfected/untreated (CTRL), uninfected/treated (GAL), infected/untreated (INF), and infected/treated (GAL/INF). The infected groups were inoculated intraperitoneally with 0.1 ml of mouse blood containing 5 × 104 trypomastigote forms of the T. cruzi QM2 strain. The galantamine-treated groups received 5 mg/kg of galantamine orally, through pipetting. From each subgroup, the parameters of parasitemia, histopathological analysis, butyrylcholinesterase activity (BuChE), and functional study of the colon were evaluated. RESULTS: BuChE performance was observed when AChE was suppressed, with increased activity in the GAL/INF group similar to the INF group on the 30th day post infection, thus corroborating the absence of a significant difference in parasitic curves and histopathological analysis. CONCLUSIONS: The presence of an inflammatory process and nests of amastigotes, as well as evidence of reactivity to ACh and NOR, suggest that galantamine did not interfere with the colonic inflammatory response or even in colonic tissue parasitism at this stage of Chagas disease.Sociedade Brasileira de Medicina Tropical - SBMT2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100339Revista da Sociedade Brasileira de Medicina Tropical v.54 2021reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0201-2021info:eu-repo/semantics/openAccessCamargo,Lucas FadelPinheiro,Guilherme DonzaliskyOliveira,Priscilla Bianca deLosada,Daniele MoraesChagas,Eduardo Federighi BaisiSperança,Márcia AparecidaChies,Agnaldo BrunoSpadella,Maria AngélicaMartins,Luciamáre Perinetti Alveseng2021-11-23T00:00:00Zoai:scielo:S0037-86822021000100339Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2021-11-23T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
title |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
spellingShingle |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain Camargo,Lucas Fadel Trypanosoma cruzi Acethylcholinesterase Butyrylcholinesterase Inflammatory process Colon Chagas disease |
title_short |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
title_full |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
title_fullStr |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
title_full_unstemmed |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
title_sort |
Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
author |
Camargo,Lucas Fadel |
author_facet |
Camargo,Lucas Fadel Pinheiro,Guilherme Donzalisky Oliveira,Priscilla Bianca de Losada,Daniele Moraes Chagas,Eduardo Federighi Baisi Sperança,Márcia Aparecida Chies,Agnaldo Bruno Spadella,Maria Angélica Martins,Luciamáre Perinetti Alves |
author_role |
author |
author2 |
Pinheiro,Guilherme Donzalisky Oliveira,Priscilla Bianca de Losada,Daniele Moraes Chagas,Eduardo Federighi Baisi Sperança,Márcia Aparecida Chies,Agnaldo Bruno Spadella,Maria Angélica Martins,Luciamáre Perinetti Alves |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Camargo,Lucas Fadel Pinheiro,Guilherme Donzalisky Oliveira,Priscilla Bianca de Losada,Daniele Moraes Chagas,Eduardo Federighi Baisi Sperança,Márcia Aparecida Chies,Agnaldo Bruno Spadella,Maria Angélica Martins,Luciamáre Perinetti Alves |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Acethylcholinesterase Butyrylcholinesterase Inflammatory process Colon Chagas disease |
topic |
Trypanosoma cruzi Acethylcholinesterase Butyrylcholinesterase Inflammatory process Colon Chagas disease |
description |
Abstract INTRODUCTION: Trypanosoma cruzi infection triggers an inflammatory process with exacerbated production of cytokines that stimulate inflammatory and anti-inflammatory signals, including the efferent anti-inflammatory signal known as the anti-inflammatory cholinergic pathway. Thus, the use of anticholinesterase drugs, such as galantamine, could minimize the inflammatory process caused by this disease. METHODS For the study at 30, 60, and 90 days, 120 Swiss mice were divided into three groups. Each group was subdivided into four subgroups: uninfected/untreated (CTRL), uninfected/treated (GAL), infected/untreated (INF), and infected/treated (GAL/INF). The infected groups were inoculated intraperitoneally with 0.1 ml of mouse blood containing 5 × 104 trypomastigote forms of the T. cruzi QM2 strain. The galantamine-treated groups received 5 mg/kg of galantamine orally, through pipetting. From each subgroup, the parameters of parasitemia, histopathological analysis, butyrylcholinesterase activity (BuChE), and functional study of the colon were evaluated. RESULTS: BuChE performance was observed when AChE was suppressed, with increased activity in the GAL/INF group similar to the INF group on the 30th day post infection, thus corroborating the absence of a significant difference in parasitic curves and histopathological analysis. CONCLUSIONS: The presence of an inflammatory process and nests of amastigotes, as well as evidence of reactivity to ACh and NOR, suggest that galantamine did not interfere with the colonic inflammatory response or even in colonic tissue parasitism at this stage of Chagas disease. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100339 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100339 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0037-8682-0201-2021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.54 2021 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122162912690176 |