Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1163 |
Resumo: | This study aimed to select Metarhizium spp. isolates to control Rhipicephalus microplus ticks by analyzing their in vitro virulence against R. microplus eggs, larvae, and engorged females as well as their ability to produce aerial conidia on potato dextrose agar (PDA). After the treatment of R. microplus eggs with the highest fungal concentration (108 conidia.ml-1), most of the eleven tested isolates reduced the larval hatching compared to the control group, except M. anisopliae s.l. ARSEF 2211 and ARSEF 3641. M. anisopliae s.l. isolates ARSEF 729, ARSEF 760, ARSEF 929, and ARSEF 3643 exhibited the best results in the egg bioassay. In the bioassay with larvae, the entomopathogenic fungal isolates yielded average larval mortality ranging from 0.1% to 98.9% and from 23.9% to 99.9% five and fifteen days after the treatment, respectively. ARSEF 552, ARSEF 729, ARSEF 929, and ARSEF 3643 yielded the highest larval mortality. Analysis of the bioassay with R. microplus engorged females found that the different isolates negatively impacted the egg mass weight, larval hatching percent, egg production index, and nutritional index. The percent of tick control ranged from 5.32% to 70.83%, and the best tick control rates were caused by M. anisopliae s.l. ARSEF 3643 (70.83%), ARSEF 3641 (62.87%), and ARSEF 729 (64.27%). The highest conidiogenesis on PDA was observed for M. anisopliae s.l. ARSEF 3641 and M. pingshaense ARSEF 552. The isolates ARSEF 729 and ARSEF 3643 are considered promising candidates for field applications against R. microplus ticks. |
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Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesisSeleção de isolados Brasileiros de Metarhizium spp. para controle do carrapato Rhipicephalus microplus: testes de virulência in vitro e conidiogênesecontrole biológico, fungos entomopatogênicos, carrapato de bovinos.biological control, entomopathogenic fungi, cattle tick.This study aimed to select Metarhizium spp. isolates to control Rhipicephalus microplus ticks by analyzing their in vitro virulence against R. microplus eggs, larvae, and engorged females as well as their ability to produce aerial conidia on potato dextrose agar (PDA). After the treatment of R. microplus eggs with the highest fungal concentration (108 conidia.ml-1), most of the eleven tested isolates reduced the larval hatching compared to the control group, except M. anisopliae s.l. ARSEF 2211 and ARSEF 3641. M. anisopliae s.l. isolates ARSEF 729, ARSEF 760, ARSEF 929, and ARSEF 3643 exhibited the best results in the egg bioassay. In the bioassay with larvae, the entomopathogenic fungal isolates yielded average larval mortality ranging from 0.1% to 98.9% and from 23.9% to 99.9% five and fifteen days after the treatment, respectively. ARSEF 552, ARSEF 729, ARSEF 929, and ARSEF 3643 yielded the highest larval mortality. Analysis of the bioassay with R. microplus engorged females found that the different isolates negatively impacted the egg mass weight, larval hatching percent, egg production index, and nutritional index. The percent of tick control ranged from 5.32% to 70.83%, and the best tick control rates were caused by M. anisopliae s.l. ARSEF 3643 (70.83%), ARSEF 3641 (62.87%), and ARSEF 729 (64.27%). The highest conidiogenesis on PDA was observed for M. anisopliae s.l. ARSEF 3641 and M. pingshaense ARSEF 552. The isolates ARSEF 729 and ARSEF 3643 are considered promising candidates for field applications against R. microplus ticks.Este estudo teve como objetivo selecionar isolados de Metarhizium spp. para controlar carrapatos Rhipicephalus microplus por meio da análise de sua virulência in vitro contra ovos, larvas e fêmeas ingurgitadas, bem como sua capacidade de produzir conídios aéreos em ágar dextrose de batata (PDA). Após o tratamento dos ovos de R. microplus com maior concentração fúngica (108 conídios.ml-1), a maioria dos onze isolados testados reduziu a eclosão larval em relação ao grupo controle, exceto M. anisopliae s.l. ARSEF 2211 e ARSEF 3641. M. anisopliae s.l. os isolados ARSEF 729, ARSEF 760, ARSEF 929 e ARSEF 3643 exibiram os melhores resultados no bioensaio com ovo. No bioensaio com larvas, os isolados fúngicos entomopatogênicos apresentaram mortalidade larval média variando de 0,1% a 98,9% e de 23,9% a 99,9% cinco e quinze dias após o tratamento, respectivamente. ARSEF 552, ARSEF 729, ARSEF 929 e ARSEF 3643 produziram a maior mortalidade larval. A análise do bioensaio com fêmeas ingurgitadas de R. microplus mostrou que os diferentes isolados impactaram negativamente o peso da massa de ovos, a porcentagem de incubação larval, o índice de produção de ovos e o índice nutricional. A porcentagem de controle de carrapatos variou de 5,32% a 70,83%, e as melhores taxas de controle de carrapatos foram causadas por M. anisopliae s.l. ARSEF 3643 (70,83%), ARSEF 3641 (62,87%) e ARSEF 729 (64,27%). A maior conidiogênese em PDA foi observada para M. anisopliae s.l. ARSEF 3641 e M. pingshaense ARSEF 552. Os isolados ARSEF 729 e ARSEF 3643Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-03-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdftext/xmlhttps://rbmv.org/BJVM/article/view/116310.29374/2527-2179.bjvm002020Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e002020Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e0020202527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1163/1066https://rbmv.org/BJVM/article/view/1163/1205Copyright (c) 2021 Giselle Arieiro Jones, Wendell Marcelo de Souza Perinotto, Mariana Guedes Camargo, Patrícia Silva Golo, Vania Rita Elias Pinheiro Bittencourthttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessArieiro Jones, Giselle de Souza Perinotto, Wendell Marcelo Guedes Camargo, Mariana Silva Golo, Patrícia Elias Pinheiro Bittencourt, Vania Rita 2022-06-26T21:50:40Zoai:ojs.rbmv.org:article/1163Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2022-06-26T21:50:40Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis Seleção de isolados Brasileiros de Metarhizium spp. para controle do carrapato Rhipicephalus microplus: testes de virulência in vitro e conidiogênese |
title |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis |
spellingShingle |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis Arieiro Jones, Giselle controle biológico, fungos entomopatogênicos, carrapato de bovinos. biological control, entomopathogenic fungi, cattle tick. |
title_short |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis |
title_full |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis |
title_fullStr |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis |
title_full_unstemmed |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis |
title_sort |
Selection of Metarhizium spp. Brazilian isolates to control Rhipicephalus microplus ticks: in vitro virulence tests and conidiogenesis |
author |
Arieiro Jones, Giselle |
author_facet |
Arieiro Jones, Giselle de Souza Perinotto, Wendell Marcelo Guedes Camargo, Mariana Silva Golo, Patrícia Elias Pinheiro Bittencourt, Vania Rita |
author_role |
author |
author2 |
de Souza Perinotto, Wendell Marcelo Guedes Camargo, Mariana Silva Golo, Patrícia Elias Pinheiro Bittencourt, Vania Rita |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Arieiro Jones, Giselle de Souza Perinotto, Wendell Marcelo Guedes Camargo, Mariana Silva Golo, Patrícia Elias Pinheiro Bittencourt, Vania Rita |
dc.subject.por.fl_str_mv |
controle biológico, fungos entomopatogênicos, carrapato de bovinos. biological control, entomopathogenic fungi, cattle tick. |
topic |
controle biológico, fungos entomopatogênicos, carrapato de bovinos. biological control, entomopathogenic fungi, cattle tick. |
description |
This study aimed to select Metarhizium spp. isolates to control Rhipicephalus microplus ticks by analyzing their in vitro virulence against R. microplus eggs, larvae, and engorged females as well as their ability to produce aerial conidia on potato dextrose agar (PDA). After the treatment of R. microplus eggs with the highest fungal concentration (108 conidia.ml-1), most of the eleven tested isolates reduced the larval hatching compared to the control group, except M. anisopliae s.l. ARSEF 2211 and ARSEF 3641. M. anisopliae s.l. isolates ARSEF 729, ARSEF 760, ARSEF 929, and ARSEF 3643 exhibited the best results in the egg bioassay. In the bioassay with larvae, the entomopathogenic fungal isolates yielded average larval mortality ranging from 0.1% to 98.9% and from 23.9% to 99.9% five and fifteen days after the treatment, respectively. ARSEF 552, ARSEF 729, ARSEF 929, and ARSEF 3643 yielded the highest larval mortality. Analysis of the bioassay with R. microplus engorged females found that the different isolates negatively impacted the egg mass weight, larval hatching percent, egg production index, and nutritional index. The percent of tick control ranged from 5.32% to 70.83%, and the best tick control rates were caused by M. anisopliae s.l. ARSEF 3643 (70.83%), ARSEF 3641 (62.87%), and ARSEF 729 (64.27%). The highest conidiogenesis on PDA was observed for M. anisopliae s.l. ARSEF 3641 and M. pingshaense ARSEF 552. The isolates ARSEF 729 and ARSEF 3643 are considered promising candidates for field applications against R. microplus ticks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1163 10.29374/2527-2179.bjvm002020 |
url |
https://rbmv.org/BJVM/article/view/1163 |
identifier_str_mv |
10.29374/2527-2179.bjvm002020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1163/1066 https://rbmv.org/BJVM/article/view/1163/1205 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/xml |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 43 No. 1 (2021); e002020 Revista Brasileira de Medicina Veterinária; v. 43 n. 1 (2021); e002020 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313110735421440 |