Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1135 |
Resumo: | This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride. |
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Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb woundsEfeitos do óleo de semente de girassol puro e ozonizado (Helianthus annuus) na formação de tecido de granulação, infecção e cicatrização de feridas nos membros locomotores de equinosferida, cicatrização, equino, fitoterápico, ozôniowound, healing, equine, phytotherapy, ozone.This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride.Este estudo foi realizado para avaliar os efeitos terapêuticos da aplicação tópica de óleo de semente de girassol puro (grupo óleo) e óleo de semente de girassol ozonizado (grupo ozônio) na cicatrização cutânea em oito equinos saudáveis. As feridas do grupo controle foram tratadas com solução de cloreto de sódio 0,9%. Duas feridas foram produzidas cirurgicamente em cada cavalo na epífise proximal do metacarpo no aspecto dorsal de cada membro anterior. A área da ferida e a contração foram medidas nos dias 0, 3, 7, 14 e 21. Foram coletadas amostras de biópsia para avaliar hiperplasia epitelial, inflamação, tecido de granulação exuberante, tipo de exsudato, angiogênese e fibroplasia. Swabs de cultura também foram coletadas. A contração da ferida aos 21 dias no grupo óleo foi de 52,68%, no grupo ozônio foi de 72,16% e no grupo controle (cloreto de sódio a 0,9%) foi de 34,80%. O tamanho e área residual da ferida, do grupo ozônio foram significativamente menores do que o grupo controle e óleo nos dias 14 e 21. As feridas do grupo controle e do óleo puro apresentaram exsudato supurativo e presença de Streptococcus zooepidemicus. Infiltrado inflamatório neutrofílico foi observado em todos os grupos. Tecido de granulação foi observado no dia 7 em todos os grupos, mas a presença de tecido de granulação exuberante foi observada apenas no grupo controle nos dias 14 e 21. Foi observada reepitelização no dia 14 no grupo ozônio. Em conclusão, esses resultados demonstram que a aplicação tópica de óleo de semente de girassol ozonizado acelera o reparo cutâneo agudo de feridas em equinos, prevenindo o excesso de tecido de granulação e infecções, mostrando-se superior ao tratamento com óleo de semente de girassol puro.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/113510.29374/2527-2179.bjvm1115321Brazilian Journal of Veterinary Medicine; Vol. 42 No. 1 (2020); e113520Revista Brasileira de Medicina Veterinária; v. 42 n. 1 (2020); e1135202527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1135/1019Copyright (c) 2020 Paula Alessandra Di Filippo, Luiza M.F. Ribeiro, Francielli P. Gobbi, Gabriela B. Lemos, Rachel Bittencourt, Hassan Jerdy, Larissa C. Silva, Inácio S. Viana, Célia R. Quirinohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDi Filippo, Paula AlessandraFeitosa Ribeiro, Luiza Maria Pereira Gobbi, Francielli Bravim Lemos, Gabriela Bittencourt Ribeiro, Rachel Jerdy, HassanCarvalho da Silva, Larissa Silva Viana, Inácio Raquel Quirino, Célia 2021-05-13T13:46:40Zoai:ojs.rbmv.org:article/1135Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2021-05-13T13:46:40Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds Efeitos do óleo de semente de girassol puro e ozonizado (Helianthus annuus) na formação de tecido de granulação, infecção e cicatrização de feridas nos membros locomotores de equinos |
title |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds |
spellingShingle |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds Di Filippo, Paula Alessandra ferida, cicatrização, equino, fitoterápico, ozônio wound, healing, equine, phytotherapy, ozone. |
title_short |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds |
title_full |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds |
title_fullStr |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds |
title_full_unstemmed |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds |
title_sort |
Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds |
author |
Di Filippo, Paula Alessandra |
author_facet |
Di Filippo, Paula Alessandra Feitosa Ribeiro, Luiza Maria Pereira Gobbi, Francielli Bravim Lemos, Gabriela Bittencourt Ribeiro, Rachel Jerdy, Hassan Carvalho da Silva, Larissa Silva Viana, Inácio Raquel Quirino, Célia |
author_role |
author |
author2 |
Feitosa Ribeiro, Luiza Maria Pereira Gobbi, Francielli Bravim Lemos, Gabriela Bittencourt Ribeiro, Rachel Jerdy, Hassan Carvalho da Silva, Larissa Silva Viana, Inácio Raquel Quirino, Célia |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Di Filippo, Paula Alessandra Feitosa Ribeiro, Luiza Maria Pereira Gobbi, Francielli Bravim Lemos, Gabriela Bittencourt Ribeiro, Rachel Jerdy, Hassan Carvalho da Silva, Larissa Silva Viana, Inácio Raquel Quirino, Célia |
dc.subject.por.fl_str_mv |
ferida, cicatrização, equino, fitoterápico, ozônio wound, healing, equine, phytotherapy, ozone. |
topic |
ferida, cicatrização, equino, fitoterápico, ozônio wound, healing, equine, phytotherapy, ozone. |
description |
This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1135 10.29374/2527-2179.bjvm1115321 |
url |
https://rbmv.org/BJVM/article/view/1135 |
identifier_str_mv |
10.29374/2527-2179.bjvm1115321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1135/1019 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 42 No. 1 (2020); e113520 Revista Brasileira de Medicina Veterinária; v. 42 n. 1 (2020); e113520 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313110691381248 |