Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds

Detalhes bibliográficos
Autor(a) principal: Di Filippo, Paula Alessandra
Data de Publicação: 2021
Outros Autores: Feitosa Ribeiro, Luiza Maria, Pereira Gobbi, Francielli, Bravim Lemos, Gabriela, Bittencourt Ribeiro, Rachel, Jerdy, Hassan, Carvalho da Silva, Larissa, Silva Viana, Inácio, Raquel Quirino, Célia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/1135
Resumo: This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride.
id SBMV-1_3c7dfdbb4ccb3eb2bf12c6037721cc15
oai_identifier_str oai:ojs.rbmv.org:article/1135
network_acronym_str SBMV-1
network_name_str Brazilian Journal of Veterinary Medicine
repository_id_str
spelling Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb woundsEfeitos do óleo de semente de girassol puro e ozonizado (Helianthus annuus) na formação de tecido de granulação, infecção e cicatrização de feridas nos membros locomotores de equinosferida, cicatrização, equino, fitoterápico, ozôniowound, healing, equine, phytotherapy, ozone.This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride.Este estudo foi realizado para avaliar os efeitos terapêuticos da aplicação tópica de óleo de semente de girassol puro (grupo óleo) e óleo de semente de girassol ozonizado (grupo ozônio) na cicatrização cutânea em oito equinos saudáveis. As feridas do grupo controle foram tratadas com solução de cloreto de sódio 0,9%. Duas feridas foram produzidas cirurgicamente em cada cavalo na epífise proximal do metacarpo no aspecto dorsal de cada membro anterior. A área da ferida e a contração foram medidas nos dias 0, 3, 7, 14 e 21. Foram coletadas amostras de biópsia para avaliar hiperplasia epitelial, inflamação, tecido de granulação exuberante, tipo de exsudato, angiogênese e fibroplasia. Swabs de cultura também foram coletadas. A contração da ferida aos 21 dias no grupo óleo foi de 52,68%, no grupo ozônio foi de 72,16% e no grupo controle (cloreto de sódio a 0,9%) foi de 34,80%. O tamanho e área residual da ferida, do grupo ozônio foram significativamente menores do que o grupo controle e óleo nos dias 14 e 21. As feridas do grupo controle e do óleo puro apresentaram exsudato supurativo e presença de Streptococcus zooepidemicus. Infiltrado inflamatório neutrofílico foi observado em todos os grupos. Tecido de granulação foi observado no dia 7 em todos os grupos, mas a presença de tecido de granulação exuberante foi observada apenas no grupo controle nos dias 14 e 21. Foi observada reepitelização no dia 14 no grupo ozônio. Em conclusão, esses resultados demonstram que a aplicação tópica de óleo de semente de girassol ozonizado acelera o reparo cutâneo agudo de feridas em equinos, prevenindo o excesso de tecido de granulação e infecções, mostrando-se superior ao tratamento com óleo de semente de girassol puro.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/113510.29374/2527-2179.bjvm1115321Brazilian Journal of Veterinary Medicine; Vol. 42 No. 1 (2020); e113520Revista Brasileira de Medicina Veterinária; v. 42 n. 1 (2020); e1135202527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1135/1019Copyright (c) 2020 Paula Alessandra Di Filippo, Luiza M.F. Ribeiro, Francielli P. Gobbi, Gabriela B. Lemos, Rachel Bittencourt, Hassan Jerdy, Larissa C. Silva, Inácio S. Viana, Célia R. Quirinohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDi Filippo, Paula AlessandraFeitosa Ribeiro, Luiza Maria Pereira Gobbi, Francielli Bravim Lemos, Gabriela Bittencourt Ribeiro, Rachel Jerdy, HassanCarvalho da Silva, Larissa Silva Viana, Inácio Raquel Quirino, Célia 2021-05-13T13:46:40Zoai:ojs.rbmv.org:article/1135Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2021-05-13T13:46:40Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
Efeitos do óleo de semente de girassol puro e ozonizado (Helianthus annuus) na formação de tecido de granulação, infecção e cicatrização de feridas nos membros locomotores de equinos
title Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
spellingShingle Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
Di Filippo, Paula Alessandra
ferida, cicatrização, equino, fitoterápico, ozônio
wound, healing, equine, phytotherapy, ozone.
title_short Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
title_full Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
title_fullStr Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
title_full_unstemmed Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
title_sort Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds
author Di Filippo, Paula Alessandra
author_facet Di Filippo, Paula Alessandra
Feitosa Ribeiro, Luiza Maria
Pereira Gobbi, Francielli
Bravim Lemos, Gabriela
Bittencourt Ribeiro, Rachel
Jerdy, Hassan
Carvalho da Silva, Larissa
Silva Viana, Inácio
Raquel Quirino, Célia
author_role author
author2 Feitosa Ribeiro, Luiza Maria
Pereira Gobbi, Francielli
Bravim Lemos, Gabriela
Bittencourt Ribeiro, Rachel
Jerdy, Hassan
Carvalho da Silva, Larissa
Silva Viana, Inácio
Raquel Quirino, Célia
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Di Filippo, Paula Alessandra
Feitosa Ribeiro, Luiza Maria
Pereira Gobbi, Francielli
Bravim Lemos, Gabriela
Bittencourt Ribeiro, Rachel
Jerdy, Hassan
Carvalho da Silva, Larissa
Silva Viana, Inácio
Raquel Quirino, Célia
dc.subject.por.fl_str_mv ferida, cicatrização, equino, fitoterápico, ozônio
wound, healing, equine, phytotherapy, ozone.
topic ferida, cicatrização, equino, fitoterápico, ozônio
wound, healing, equine, phytotherapy, ozone.
description This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/1135
10.29374/2527-2179.bjvm1115321
url https://rbmv.org/BJVM/article/view/1135
identifier_str_mv 10.29374/2527-2179.bjvm1115321
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/1135/1019
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 42 No. 1 (2020); e113520
Revista Brasileira de Medicina Veterinária; v. 42 n. 1 (2020); e113520
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
_version_ 1798313110691381248