Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/352 |
Resumo: | ABSTRACT. Viscardi V., Lopes A.C.G., Beling J.C.F., Gioia G.V., Torres Filhos R.A., Lessa D.A.B. & Alencar N.X. Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines. [Efeito da infusão única e em duplicata de 250mL de solução salina na avaliação citológica do lavado broncoalveolar em equinos.] Revista Brasileira de Medicina Veterinária, 37(1):33-35, 2015. Departamento de Patologia e Clí- nica Veterinária, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil. E-mail: nayro@vm.uff.br The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Different volumes of infusions are employed to obtain BALs in horses, which can influence the cytology. Therein, the present paper intends to determine the effects of a single and a duplicate infusion of 250 mL of saline solution in BAL cell type count. BAL samples of 30 equines obtained with the infusion of saline solution divided into two aliquots of 250mL (A1 and A2) were used. The cytological preparations were done by cytocentrifugation and all glass microscope slides were fixed and stained with Giemsa for the differential cell count. As for the effect of different infusion volumes (A1 and A2) on the cell type count, there were significant differences only among neutrophil percentages (p=0,0236), that was lower in the latter samples (A2). Although the infusion of 250mL (A1) allows the obtaining of a representative sample of cell population in the lower respiratory tract, it is not appropriate to compare these results to currently accepted reference values, making it necessary to establish reference values for this volume. |
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Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equinesEfeito da infusão única e em duplicata de 250mL de solução salina na avaliação citológica do lavado broncoalveolar em equinosCavaloscitologia broncoalveolarvolume de infusãoHorsecytologybronchoalveolar lavageinfusion volumeABSTRACT. Viscardi V., Lopes A.C.G., Beling J.C.F., Gioia G.V., Torres Filhos R.A., Lessa D.A.B. & Alencar N.X. Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines. [Efeito da infusão única e em duplicata de 250mL de solução salina na avaliação citológica do lavado broncoalveolar em equinos.] Revista Brasileira de Medicina Veterinária, 37(1):33-35, 2015. Departamento de Patologia e Clí- nica Veterinária, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil. E-mail: nayro@vm.uff.br The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Different volumes of infusions are employed to obtain BALs in horses, which can influence the cytology. Therein, the present paper intends to determine the effects of a single and a duplicate infusion of 250 mL of saline solution in BAL cell type count. BAL samples of 30 equines obtained with the infusion of saline solution divided into two aliquots of 250mL (A1 and A2) were used. The cytological preparations were done by cytocentrifugation and all glass microscope slides were fixed and stained with Giemsa for the differential cell count. As for the effect of different infusion volumes (A1 and A2) on the cell type count, there were significant differences only among neutrophil percentages (p=0,0236), that was lower in the latter samples (A2). Although the infusion of 250mL (A1) allows the obtaining of a representative sample of cell population in the lower respiratory tract, it is not appropriate to compare these results to currently accepted reference values, making it necessary to establish reference values for this volume.O lavado broncoalveolar (LBA) é um método sensível de diagnóstico das enfermidades da porção distal do trato respiratório posterior e tem sido amplamente utilizado por diversos pesquisadores. Diferentes volumes de infusão são aplicados para obter o LBA de cavalos, o que pode interferir na contagem citologia diferencial. Dentro deste contexto, este trabalho estudou o efeito da infusão única e em duplicata de 250mL de solução salina na citologia do LBA de equinos. Amostras de LBA de 30 equinos adultos foram obtidas com a infusão de solução salina, dividida em duas alí- quotas de 250mL (A1 e A2). As preparações citológicas foram feitas por citocentrifugação e fixadas e coradas com Giemsa para a realização da contagem diferencial dos tipos celulares. Foi observada diferença estatisticamente significativa (p=0,0236) em relação aos neutrófilos, cuja percentagem foi menor nas amostras A2. Apesar da infusão única de 250mL (A1) possibilitar a obtenção de amostras representativas da população celular do trato respiratório posterior, não é apropriado comparar estes resultados com aqueles comumente utilizados como valores de referência, sendo necessário estabelecer novos valores de referência para este volume de infusão.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2015-03-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfapplication/pdfhttps://rbmv.org/BJVM/article/view/352Brazilian Journal of Veterinary Medicine; Vol. 37 No. 1 (2015); 33-35Revista Brasileira de Medicina Veterinária; v. 37 n. 1 (2015); 33-352527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/352/250https://rbmv.org/BJVM/article/view/352/835Viscardi, VanessaLopes, Aline del Carmen GarciaBeling, Joana de Castro FariaGioia, Gisela VasconcelosFilho, Rodolpho de Almeida TorresLessa, Daniel Augusto Barrosode Alencar, Nayro Xavierinfo:eu-repo/semantics/openAccess2020-12-23T17:33:00Zoai:ojs.rbmv.org:article/352Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:33Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines Efeito da infusão única e em duplicata de 250mL de solução salina na avaliação citológica do lavado broncoalveolar em equinos |
title |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines |
spellingShingle |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines Viscardi, Vanessa Cavalos citologia broncoalveolar volume de infusão Horse cytology bronchoalveolar lavage infusion volume |
title_short |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines |
title_full |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines |
title_fullStr |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines |
title_full_unstemmed |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines |
title_sort |
Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines |
author |
Viscardi, Vanessa |
author_facet |
Viscardi, Vanessa Lopes, Aline del Carmen Garcia Beling, Joana de Castro Faria Gioia, Gisela Vasconcelos Filho, Rodolpho de Almeida Torres Lessa, Daniel Augusto Barroso de Alencar, Nayro Xavier |
author_role |
author |
author2 |
Lopes, Aline del Carmen Garcia Beling, Joana de Castro Faria Gioia, Gisela Vasconcelos Filho, Rodolpho de Almeida Torres Lessa, Daniel Augusto Barroso de Alencar, Nayro Xavier |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Viscardi, Vanessa Lopes, Aline del Carmen Garcia Beling, Joana de Castro Faria Gioia, Gisela Vasconcelos Filho, Rodolpho de Almeida Torres Lessa, Daniel Augusto Barroso de Alencar, Nayro Xavier |
dc.subject.por.fl_str_mv |
Cavalos citologia broncoalveolar volume de infusão Horse cytology bronchoalveolar lavage infusion volume |
topic |
Cavalos citologia broncoalveolar volume de infusão Horse cytology bronchoalveolar lavage infusion volume |
description |
ABSTRACT. Viscardi V., Lopes A.C.G., Beling J.C.F., Gioia G.V., Torres Filhos R.A., Lessa D.A.B. & Alencar N.X. Effects of single and duplicate infusions of 250mL of saline solution in the cytological evaluation of bronchoalveolar lavage in equines. [Efeito da infusão única e em duplicata de 250mL de solução salina na avaliação citológica do lavado broncoalveolar em equinos.] Revista Brasileira de Medicina Veterinária, 37(1):33-35, 2015. Departamento de Patologia e Clí- nica Veterinária, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho 64, Niterói, RJ 24230-340, Brazil. E-mail: nayro@vm.uff.br The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Different volumes of infusions are employed to obtain BALs in horses, which can influence the cytology. Therein, the present paper intends to determine the effects of a single and a duplicate infusion of 250 mL of saline solution in BAL cell type count. BAL samples of 30 equines obtained with the infusion of saline solution divided into two aliquots of 250mL (A1 and A2) were used. The cytological preparations were done by cytocentrifugation and all glass microscope slides were fixed and stained with Giemsa for the differential cell count. As for the effect of different infusion volumes (A1 and A2) on the cell type count, there were significant differences only among neutrophil percentages (p=0,0236), that was lower in the latter samples (A2). Although the infusion of 250mL (A1) allows the obtaining of a representative sample of cell population in the lower respiratory tract, it is not appropriate to compare these results to currently accepted reference values, making it necessary to establish reference values for this volume. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/352 |
url |
https://rbmv.org/BJVM/article/view/352 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/352/250 https://rbmv.org/BJVM/article/view/352/835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 37 No. 1 (2015); 33-35 Revista Brasileira de Medicina Veterinária; v. 37 n. 1 (2015); 33-35 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313107550896128 |