Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology

Detalhes bibliográficos
Autor(a) principal: Viscardi, Vanessa
Data de Publicação: 2013
Outros Autores: Belling, Joana de Castro Faria, Lopes, Aline Del Carmen Garcia, Gioia, Gisela Vasconcelos, Torres Filho, Rodolpho de Almeida, Alencar, Nayro Xavier de, Lessa, Daniel Augusto Barroso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/55840
Resumo: The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Cytocentrifugation is the choice cytological preparation technique, but demands specific and costly equipment. Therefore, the present paper intends to verify the applicability of the linear smear technique to evaluate BAL samples. For this, BAL samples of 30 equines were used and the cytological preparations were done by cytocentrifugation and linear smear techniques. All glass microscope slides were fixed and stained with Giemsa for the differential cell count. Regarding the effect of the preparation technique on differential counts, no significant difference in any cell type was found. The linear smear is a reliable alternative and can be recommended as a substitution to cytocentrifugation.
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spelling Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytologyComparação entre as técnicas de esfregaço linear e citocentrifugação para avaliação citológica do lavado broncoalveolar equinoCavaloLavado broncoalveolarCitocentrifugaçãoEsfregaço linearHorseBronchoalveolar lavageCytocentrifugationLinear smearThe bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Cytocentrifugation is the choice cytological preparation technique, but demands specific and costly equipment. Therefore, the present paper intends to verify the applicability of the linear smear technique to evaluate BAL samples. For this, BAL samples of 30 equines were used and the cytological preparations were done by cytocentrifugation and linear smear techniques. All glass microscope slides were fixed and stained with Giemsa for the differential cell count. Regarding the effect of the preparation technique on differential counts, no significant difference in any cell type was found. The linear smear is a reliable alternative and can be recommended as a substitution to cytocentrifugation.O lavado broncoalveolar (LBA) é um método sensível para diagnosticar doenças do trato respiratório posterior e vem sendo utilizado por diversos esquisadores. A citocentrifugação, técnica de escolha para processar amostras citológicas de LBA, exige equipamentos específicos e caros. Por isso, este trabalho verificou a aplicabilidade da técnica de esfregaço linear para avaliação citológica do LBA. Foram utilizadas amostras de LBA de 30 equinos adultos. As preparações citológicas foram realizadas tanto por citocentrifugação quanto por esfregaço linear. Todas as lâminas foram fixadas e coradas com Giemsa para realização da contagem celular diferencial. Não foram encontradas alterações morfológicas significativas e nem diferenças estatísticas entre nenhum dos tipos celulares processados pelos dois métodos, o que permite afirmar que o método de esfregaço linear é uma alternativa segura para avaliação morfológica celular do LBA de equinos, podendo ser utilizado no lugar da citocentrifugação quando esta não estiver disponível.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2013-02-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5584010.11606/issn.2318-3659.v50i1p68-70Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 1 (2013); 68-70Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 1 (2013); 68-70Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 1 (2013); 68-70Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 1 (2013); 68-701678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/55840/59245Viscardi, VanessaBelling, Joana de Castro FariaLopes, Aline Del Carmen GarciaGioia, Gisela VasconcelosTorres Filho, Rodolpho de AlmeidaAlencar, Nayro Xavier deLessa, Daniel Augusto Barrosoinfo:eu-repo/semantics/openAccess2020-06-23T04:07:34Zoai:revistas.usp.br:article/55840Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:32.360869Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
Comparação entre as técnicas de esfregaço linear e citocentrifugação para avaliação citológica do lavado broncoalveolar equino
title Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
spellingShingle Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
Viscardi, Vanessa
Cavalo
Lavado broncoalveolar
Citocentrifugação
Esfregaço linear
Horse
Bronchoalveolar lavage
Cytocentrifugation
Linear smear
title_short Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
title_full Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
title_fullStr Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
title_full_unstemmed Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
title_sort Comparison between linear smear concentration and cytocentrifugation techniques to evaluate equine bronchoalveolar lavage cytology
author Viscardi, Vanessa
author_facet Viscardi, Vanessa
Belling, Joana de Castro Faria
Lopes, Aline Del Carmen Garcia
Gioia, Gisela Vasconcelos
Torres Filho, Rodolpho de Almeida
Alencar, Nayro Xavier de
Lessa, Daniel Augusto Barroso
author_role author
author2 Belling, Joana de Castro Faria
Lopes, Aline Del Carmen Garcia
Gioia, Gisela Vasconcelos
Torres Filho, Rodolpho de Almeida
Alencar, Nayro Xavier de
Lessa, Daniel Augusto Barroso
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Viscardi, Vanessa
Belling, Joana de Castro Faria
Lopes, Aline Del Carmen Garcia
Gioia, Gisela Vasconcelos
Torres Filho, Rodolpho de Almeida
Alencar, Nayro Xavier de
Lessa, Daniel Augusto Barroso
dc.subject.por.fl_str_mv Cavalo
Lavado broncoalveolar
Citocentrifugação
Esfregaço linear
Horse
Bronchoalveolar lavage
Cytocentrifugation
Linear smear
topic Cavalo
Lavado broncoalveolar
Citocentrifugação
Esfregaço linear
Horse
Bronchoalveolar lavage
Cytocentrifugation
Linear smear
description The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Cytocentrifugation is the choice cytological preparation technique, but demands specific and costly equipment. Therefore, the present paper intends to verify the applicability of the linear smear technique to evaluate BAL samples. For this, BAL samples of 30 equines were used and the cytological preparations were done by cytocentrifugation and linear smear techniques. All glass microscope slides were fixed and stained with Giemsa for the differential cell count. Regarding the effect of the preparation technique on differential counts, no significant difference in any cell type was found. The linear smear is a reliable alternative and can be recommended as a substitution to cytocentrifugation.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/55840
10.11606/issn.2318-3659.v50i1p68-70
url https://www.revistas.usp.br/bjvras/article/view/55840
identifier_str_mv 10.11606/issn.2318-3659.v50i1p68-70
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/55840/59245
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 1 (2013); 68-70
Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 1 (2013); 68-70
Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 1 (2013); 68-70
Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 1 (2013); 68-70
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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