Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic

Detalhes bibliográficos
Autor(a) principal: de Almeida, Nadia Rossi
Data de Publicação: 2014
Outros Autores: Danelli, Maria das Graças Miranda, Hagiwara, Mitika Kuribayashi, de Góes, Ana Carolina Magalhães Andrade, Medeiros, Marco Alberto, Mazur, Carlos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/663
Resumo: ABSTRACT. Almeida N.R., Danelli M.G.M., Hagiwara M.K., Góes A.C.M.A., Medeiros M.A. & Mazur C. Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic. [Avaliação de anticorpos extraídos de um novo antígeno recombinante para o diagnóstico da infecção pelo Vírus da Leucemia Felina (FeLV).] Revista Brasileira de Medicina Veterinária, 35(Supl.2):95-99, 2013. Programa de Pós-graduação em Ciências Veterinárias, Instituto de Veterinária, Anexo 1, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, Campus Universitário, Seropédica, RJ 23897-970, Brasil. E-mail: nadia.ufrrj@gmail.com A novel recombinant Feline Leukemia Virus (FeLV) capsid antigen was developed and inoculated in rabbits to elicit antibodies against it. These antibodies were evaluated by indirect immunoflourescence assay (IFA) and proved to react with the native FeLV antigen in agreement with the previous tests performed in animal samples. Considering its advantages to Brazilian scenario, the established IFA using recombinant antigen may be a practical and low cost diagnostic test to be incorpored as diagnosis of Feline Leukemia Virus.
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spelling Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnosticAvaliação de anticorpos extraídos de um novo antígeno recombinante para o diagnóstico da infecção pelo Vírus da Leucemia Felina (FeLV)FeLVgatosIFAleucemiaFeLVcatsIFAleukemiaABSTRACT. Almeida N.R., Danelli M.G.M., Hagiwara M.K., Góes A.C.M.A., Medeiros M.A. & Mazur C. Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic. [Avaliação de anticorpos extraídos de um novo antígeno recombinante para o diagnóstico da infecção pelo Vírus da Leucemia Felina (FeLV).] Revista Brasileira de Medicina Veterinária, 35(Supl.2):95-99, 2013. Programa de Pós-graduação em Ciências Veterinárias, Instituto de Veterinária, Anexo 1, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, Campus Universitário, Seropédica, RJ 23897-970, Brasil. E-mail: nadia.ufrrj@gmail.com A novel recombinant Feline Leukemia Virus (FeLV) capsid antigen was developed and inoculated in rabbits to elicit antibodies against it. These antibodies were evaluated by indirect immunoflourescence assay (IFA) and proved to react with the native FeLV antigen in agreement with the previous tests performed in animal samples. Considering its advantages to Brazilian scenario, the established IFA using recombinant antigen may be a practical and low cost diagnostic test to be incorpored as diagnosis of Feline Leukemia Virus.Um novo antígeno recombinante de capsídeo do Vírus da Leucemia Felina (FeLV) foi desenvolvido e inoculado em coelhos com o intuito de obter-se anticorpos. Estes anticorpos foram avaliados pela técnica de imunofluorescência indireta (IFA) e reagidos contra o antígeno nativo do FeLV, de acordo com os testes anteriores realizados em amostras de animais. Considerando suas vantagens no cenário brasileiro, o estabelecimento da IFA utilizando um antígeno recombinante pode ser um teste prático e de baixo custo, podendo ser incorporado como diagnóstico da Leucemia Viral Felina.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2014-03-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/663Brazilian Journal of Veterinary Medicine; Vol. 35 No. Supl.2 (2013); 95-99Revista Brasileira de Medicina Veterinária; v. 35 n. Supl.2 (2013); 95-992527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/663/523de Almeida, Nadia RossiDanelli, Maria das Graças MirandaHagiwara, Mitika Kuribayashide Góes, Ana Carolina Magalhães AndradeMedeiros, Marco AlbertoMazur, Carlosinfo:eu-repo/semantics/openAccess2020-12-23T17:31:17Zoai:ojs.rbmv.org:article/663Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:31:17Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
Avaliação de anticorpos extraídos de um novo antígeno recombinante para o diagnóstico da infecção pelo Vírus da Leucemia Felina (FeLV)
title Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
spellingShingle Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
de Almeida, Nadia Rossi
FeLV
gatos
IFA
leucemia
FeLV
cats
IFA
leukemia
title_short Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
title_full Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
title_fullStr Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
title_full_unstemmed Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
title_sort Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic
author de Almeida, Nadia Rossi
author_facet de Almeida, Nadia Rossi
Danelli, Maria das Graças Miranda
Hagiwara, Mitika Kuribayashi
de Góes, Ana Carolina Magalhães Andrade
Medeiros, Marco Alberto
Mazur, Carlos
author_role author
author2 Danelli, Maria das Graças Miranda
Hagiwara, Mitika Kuribayashi
de Góes, Ana Carolina Magalhães Andrade
Medeiros, Marco Alberto
Mazur, Carlos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv de Almeida, Nadia Rossi
Danelli, Maria das Graças Miranda
Hagiwara, Mitika Kuribayashi
de Góes, Ana Carolina Magalhães Andrade
Medeiros, Marco Alberto
Mazur, Carlos
dc.subject.por.fl_str_mv FeLV
gatos
IFA
leucemia
FeLV
cats
IFA
leukemia
topic FeLV
gatos
IFA
leucemia
FeLV
cats
IFA
leukemia
description ABSTRACT. Almeida N.R., Danelli M.G.M., Hagiwara M.K., Góes A.C.M.A., Medeiros M.A. & Mazur C. Evaluation of antibodies elicited by a new recombinant antigen for Feline Leukemia Virus (FeLV) infection diagnostic. [Avaliação de anticorpos extraídos de um novo antígeno recombinante para o diagnóstico da infecção pelo Vírus da Leucemia Felina (FeLV).] Revista Brasileira de Medicina Veterinária, 35(Supl.2):95-99, 2013. Programa de Pós-graduação em Ciências Veterinárias, Instituto de Veterinária, Anexo 1, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, Campus Universitário, Seropédica, RJ 23897-970, Brasil. E-mail: nadia.ufrrj@gmail.com A novel recombinant Feline Leukemia Virus (FeLV) capsid antigen was developed and inoculated in rabbits to elicit antibodies against it. These antibodies were evaluated by indirect immunoflourescence assay (IFA) and proved to react with the native FeLV antigen in agreement with the previous tests performed in animal samples. Considering its advantages to Brazilian scenario, the established IFA using recombinant antigen may be a practical and low cost diagnostic test to be incorpored as diagnosis of Feline Leukemia Virus.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/663
url https://rbmv.org/BJVM/article/view/663
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/663/523
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 35 No. Supl.2 (2013); 95-99
Revista Brasileira de Medicina Veterinária; v. 35 n. Supl.2 (2013); 95-99
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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