Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/637 |
Resumo: | ABSTRACT. de Moraes E.P.B.X., Freitas A.C., Costa M.M., PinheiroJr J.W. & Mota R.A. [Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep]. Utilização da PCR nested para detecção de Toxoplasma gondii no sangue de ovelhas experimentalmente infectadas. Revista Brasileira de Medicina Veterinária, 35(4):329-334, 2013. Laboratório de Doenças Infecto-Contagiosas dos Animais Domésticos, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, PE 52171-900, Brasil. E-mail: ericaxmoraes@globo.com The aim of this study was to evaluate the sensitivity of the PCR and nested PCR for T. gondii detection in the blood of experimentally infected sheep with tachyzoites through the semen. Blood samples of 41 sheep were taken, which were infected with different dosages: G1 - 6.5 X 104 tachyzoites; G2- 4 X 107 tachyzoites and G3- control group. For the anti-T.gondii antibodies research the Indirect Immunofluorescence (IIF) technique was used. For the PCR and nested PCR, primers derived from B1 gene were used. Seroconversion was observed only for the infected group. In the PCR, T. gondii DNA was detected in 40% of the infected sheep blood samples, and in the nested PCR, in 93.3% of the samples. In the G3, no antibodies were detected, as well as parasitic DNA in neither of the animals. It is possible to conclude that the nested PCR is more sensitive for the T. gondii detection in the blood of animals experimentally infected through the semen, presenting superior results in comparison to those of the PCR and might be successfully used in studies like this one. |
id |
SBMV-1_65933177bd17732efee7eaf3a99be8e0 |
---|---|
oai_identifier_str |
oai:ojs.rbmv.org:article/637 |
network_acronym_str |
SBMV-1 |
network_name_str |
Brazilian Journal of Veterinary Medicine |
repository_id_str |
|
spelling |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheepUTILIZAÇÃO DA PCR NESTED PARA DETECÇÃO DE Toxoplasma gondii NO SANGUE DE OVELHAS EXPERIMENTALMENTE INFECTADASDiagnósticotoxoplasmose ovinatécnica molecularDiagnosetoxoplasmosis in sheepmolecular techniqueABSTRACT. de Moraes E.P.B.X., Freitas A.C., Costa M.M., PinheiroJr J.W. & Mota R.A. [Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep]. Utilização da PCR nested para detecção de Toxoplasma gondii no sangue de ovelhas experimentalmente infectadas. Revista Brasileira de Medicina Veterinária, 35(4):329-334, 2013. Laboratório de Doenças Infecto-Contagiosas dos Animais Domésticos, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, PE 52171-900, Brasil. E-mail: ericaxmoraes@globo.com The aim of this study was to evaluate the sensitivity of the PCR and nested PCR for T. gondii detection in the blood of experimentally infected sheep with tachyzoites through the semen. Blood samples of 41 sheep were taken, which were infected with different dosages: G1 - 6.5 X 104 tachyzoites; G2- 4 X 107 tachyzoites and G3- control group. For the anti-T.gondii antibodies research the Indirect Immunofluorescence (IIF) technique was used. For the PCR and nested PCR, primers derived from B1 gene were used. Seroconversion was observed only for the infected group. In the PCR, T. gondii DNA was detected in 40% of the infected sheep blood samples, and in the nested PCR, in 93.3% of the samples. In the G3, no antibodies were detected, as well as parasitic DNA in neither of the animals. It is possible to conclude that the nested PCR is more sensitive for the T. gondii detection in the blood of animals experimentally infected through the semen, presenting superior results in comparison to those of the PCR and might be successfully used in studies like this one.Objetivou-se com esse estudo avaliar a sensibilidade da PCR e PCR nested na detecção de T. gondiino sangue de ovelhas experimentalmente infectadas com taquizoítos via sêmen. Foram colhidas amostras de sangue de 30 ovelhas infectadas com diferentes doses: G1- 6,5 X 104 taquizoítos; G2- 4 X 107 taquizoítos e G3- grupo controle. Para a pesquisa de anticorpos anti-T. gondii foi utilizada a técnica de Imunofluorescência Indireta (IFI). Para o PCR e PCR nested foram utilizados iniciadores derivados do gene B1. Observou-se soroconversão apenas dos grupos infectados. Na PCR, detectou-se o DNA do T. gondii em 40% e na PCR nested em 93,3% amostras de sangue das ovelhas infectadas. No G3 não foi detectado anticorpos e DNA parasitário em nenhum animal.Conclui-se que a PCR nested é mais sensível na detecção de T. gondii no sangue de animais experimentalmente infectados via sêmen, mostrando resultado superior ao da PCR e podendo ser utilizada com êxito em estudos dessa natureza.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2013-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/637Brazilian Journal of Veterinary Medicine; Vol. 35 No. 4 (2013); 329-334Revista Brasileira de Medicina Veterinária; v. 35 n. 4 (2013); 329-3342527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/637/502de Moraes, Érica Paes Barreto Xavierde Freitas, Antônio Carlosda Costa, Mateus MatiuzziJúnior, José Wilton PinheiroMota, Rinaldo Aparecidoinfo:eu-repo/semantics/openAccess2020-12-23T17:30:52Zoai:ojs.rbmv.org:article/637Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:30:52Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep UTILIZAÇÃO DA PCR NESTED PARA DETECÇÃO DE Toxoplasma gondii NO SANGUE DE OVELHAS EXPERIMENTALMENTE INFECTADAS |
title |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep |
spellingShingle |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep de Moraes, Érica Paes Barreto Xavier Diagnóstico toxoplasmose ovina técnica molecular Diagnose toxoplasmosis in sheep molecular technique |
title_short |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep |
title_full |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep |
title_fullStr |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep |
title_full_unstemmed |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep |
title_sort |
Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep |
author |
de Moraes, Érica Paes Barreto Xavier |
author_facet |
de Moraes, Érica Paes Barreto Xavier de Freitas, Antônio Carlos da Costa, Mateus Matiuzzi Júnior, José Wilton Pinheiro Mota, Rinaldo Aparecido |
author_role |
author |
author2 |
de Freitas, Antônio Carlos da Costa, Mateus Matiuzzi Júnior, José Wilton Pinheiro Mota, Rinaldo Aparecido |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
de Moraes, Érica Paes Barreto Xavier de Freitas, Antônio Carlos da Costa, Mateus Matiuzzi Júnior, José Wilton Pinheiro Mota, Rinaldo Aparecido |
dc.subject.por.fl_str_mv |
Diagnóstico toxoplasmose ovina técnica molecular Diagnose toxoplasmosis in sheep molecular technique |
topic |
Diagnóstico toxoplasmose ovina técnica molecular Diagnose toxoplasmosis in sheep molecular technique |
description |
ABSTRACT. de Moraes E.P.B.X., Freitas A.C., Costa M.M., PinheiroJr J.W. & Mota R.A. [Nested PCR utilization for Toxoplasma gondii detection in the blood of experimentally infected sheep]. Utilização da PCR nested para detecção de Toxoplasma gondii no sangue de ovelhas experimentalmente infectadas. Revista Brasileira de Medicina Veterinária, 35(4):329-334, 2013. Laboratório de Doenças Infecto-Contagiosas dos Animais Domésticos, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, PE 52171-900, Brasil. E-mail: ericaxmoraes@globo.com The aim of this study was to evaluate the sensitivity of the PCR and nested PCR for T. gondii detection in the blood of experimentally infected sheep with tachyzoites through the semen. Blood samples of 41 sheep were taken, which were infected with different dosages: G1 - 6.5 X 104 tachyzoites; G2- 4 X 107 tachyzoites and G3- control group. For the anti-T.gondii antibodies research the Indirect Immunofluorescence (IIF) technique was used. For the PCR and nested PCR, primers derived from B1 gene were used. Seroconversion was observed only for the infected group. In the PCR, T. gondii DNA was detected in 40% of the infected sheep blood samples, and in the nested PCR, in 93.3% of the samples. In the G3, no antibodies were detected, as well as parasitic DNA in neither of the animals. It is possible to conclude that the nested PCR is more sensitive for the T. gondii detection in the blood of animals experimentally infected through the semen, presenting superior results in comparison to those of the PCR and might be successfully used in studies like this one. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/637 |
url |
https://rbmv.org/BJVM/article/view/637 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/637/502 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 35 No. 4 (2013); 329-334 Revista Brasileira de Medicina Veterinária; v. 35 n. 4 (2013); 329-334 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313108824915968 |