Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro

Detalhes bibliográficos
Autor(a) principal: Assad, Renata Quintela
Data de Publicação: 2021
Outros Autores: Junior, Eloy Seabra, Lambert, Monique Moraes, Correa, Carmen Beatriz Seti, Lemos, Tatiana Didonet, Toma, Helena Keiko, Scott, Fabio Barbour, Almosny, Nadia Regina Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/321
Resumo: The canine babesiosis has a worldwide distribution, its one of the most important tick-borne disease. It is endemic in Brazil, where the prevalence is increasing in several áreas. Typically, anemia and thrombocytopenia are found in dogs with babesiosis, such as hematological changes. The aim of this study was to evaluate the piroplasms prevalence and hematologic changes in dogs at Small Animals Veterinary Hospital of Universidade Federal Rural do Rio de Janeiro (UFRRJ), through the amplification of the 18S rRNA gene using Polymerase Chain Reaction (PCR) and genetic description using sequencing. Blood samples of 250 dogs were randomly selected. From all samples, nine (3.6%) were positive for Babesia spp. using conventional PCR. The sequencing was performed on products from PCR and the positives samples were identified as Babesia vogeli. The main hematological alterations were anemia (66.7%) and thrombocytopenia (89%). The piroplasm that most affect dogs in the region is Babesia vogeli and it can develop anemia and thrombocytopenia.
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spelling Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de JaneiroDetecção molecular e alterações hematológicas de amostras de cães naturalmente infectados por Babesia vogeli na região metropolitana do Rio de JaneiropiroplasmosecarrapatoPRCprotozoarioanalise molecularpiroplasmosis, ticks, PCR, protozoon, molecular analysis.The canine babesiosis has a worldwide distribution, its one of the most important tick-borne disease. It is endemic in Brazil, where the prevalence is increasing in several áreas. Typically, anemia and thrombocytopenia are found in dogs with babesiosis, such as hematological changes. The aim of this study was to evaluate the piroplasms prevalence and hematologic changes in dogs at Small Animals Veterinary Hospital of Universidade Federal Rural do Rio de Janeiro (UFRRJ), through the amplification of the 18S rRNA gene using Polymerase Chain Reaction (PCR) and genetic description using sequencing. Blood samples of 250 dogs were randomly selected. From all samples, nine (3.6%) were positive for Babesia spp. using conventional PCR. The sequencing was performed on products from PCR and the positives samples were identified as Babesia vogeli. The main hematological alterations were anemia (66.7%) and thrombocytopenia (89%). The piroplasm that most affect dogs in the region is Babesia vogeli and it can develop anemia and thrombocytopenia.A babesiose canina é, no mundo inteiro, uma das mais importantes doenças transmitidas por carrapatos. Ela é uma doença endêmica no Brasil e sua presença tem sido detectada em várias localidades. As alterações hematológicas mais comumente encontradas em cães com babesiose são anemia e trombocitopenia. O objetivo desse trabalho foi avaliar a prevalência de piroplasmas e as alterações hematológicas encontradas em cães, da região metropolitana do Rio de Janeiro, que foram atendidos no setor de Pequenos Animais do Hospital Veterinário da Universidade Federal Rural do Rio de Janeiro (UFRRJ) através da amplificação do gene 18S rRNA pela Reação em Cadeia de Polimerase (PCR) e caracterização genética através do sequenciamento. Foram utilizadas, aleatoriamente, 250 amostras de sangue de cães. Do total de amostras, nove (3,6%) foram positivas para Babesia spp. pela PCR para piroplasmas. As amostras positivas foram sequenciadas e identificadas como Babesia vogeli. As alterações hematológicas predominantes nos animais positvos na PCR foram anemia em seis (66,7%) e trombocitopenia em oito (89%) cães. Conclui-se que Babesia vogeli é o piroplasma que mais acomete cães naquela região, podendo causar alterações como anemia e trombocitopenia nos animais acometidos.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2021-05-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/32110.29374/2527-2179.bjvm032120Brazilian Journal of Veterinary Medicine; Vol. 42 No. 1 (2020); e032120Revista Brasileira de Medicina Veterinária; v. 42 n. 1 (2020); e0321202527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/321/998Copyright (c) 2020 Renata Quintela Assad, Eloy Seabra Junior, Monique Moraes Lambert, Carmen Beatriz Seti Correa, Tatiana Didonet Lemos, Helena Keiko Toma, Fabio Barbour Scott, Nadia Regina Pereira Almosnyhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAssad, Renata QuintelaJunior, Eloy SeabraLambert, Monique MoraesCorrea, Carmen Beatriz SetiLemos, Tatiana DidonetToma, Helena KeikoScott, Fabio BarbourAlmosny, Nadia Regina Pereira2021-05-13T13:46:40Zoai:ojs.rbmv.org:article/321Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2021-05-13T13:46:40Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
Detecção molecular e alterações hematológicas de amostras de cães naturalmente infectados por Babesia vogeli na região metropolitana do Rio de Janeiro
title Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
spellingShingle Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
Assad, Renata Quintela
piroplasmose
carrapato
PRC
protozoario
analise molecular
piroplasmosis, ticks, PCR, protozoon, molecular analysis.
title_short Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
title_full Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
title_fullStr Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
title_full_unstemmed Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
title_sort Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro
author Assad, Renata Quintela
author_facet Assad, Renata Quintela
Junior, Eloy Seabra
Lambert, Monique Moraes
Correa, Carmen Beatriz Seti
Lemos, Tatiana Didonet
Toma, Helena Keiko
Scott, Fabio Barbour
Almosny, Nadia Regina Pereira
author_role author
author2 Junior, Eloy Seabra
Lambert, Monique Moraes
Correa, Carmen Beatriz Seti
Lemos, Tatiana Didonet
Toma, Helena Keiko
Scott, Fabio Barbour
Almosny, Nadia Regina Pereira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Assad, Renata Quintela
Junior, Eloy Seabra
Lambert, Monique Moraes
Correa, Carmen Beatriz Seti
Lemos, Tatiana Didonet
Toma, Helena Keiko
Scott, Fabio Barbour
Almosny, Nadia Regina Pereira
dc.subject.por.fl_str_mv piroplasmose
carrapato
PRC
protozoario
analise molecular
piroplasmosis, ticks, PCR, protozoon, molecular analysis.
topic piroplasmose
carrapato
PRC
protozoario
analise molecular
piroplasmosis, ticks, PCR, protozoon, molecular analysis.
description The canine babesiosis has a worldwide distribution, its one of the most important tick-borne disease. It is endemic in Brazil, where the prevalence is increasing in several áreas. Typically, anemia and thrombocytopenia are found in dogs with babesiosis, such as hematological changes. The aim of this study was to evaluate the piroplasms prevalence and hematologic changes in dogs at Small Animals Veterinary Hospital of Universidade Federal Rural do Rio de Janeiro (UFRRJ), through the amplification of the 18S rRNA gene using Polymerase Chain Reaction (PCR) and genetic description using sequencing. Blood samples of 250 dogs were randomly selected. From all samples, nine (3.6%) were positive for Babesia spp. using conventional PCR. The sequencing was performed on products from PCR and the positives samples were identified as Babesia vogeli. The main hematological alterations were anemia (66.7%) and thrombocytopenia (89%). The piroplasm that most affect dogs in the region is Babesia vogeli and it can develop anemia and thrombocytopenia.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/321
10.29374/2527-2179.bjvm032120
url https://rbmv.org/BJVM/article/view/321
identifier_str_mv 10.29374/2527-2179.bjvm032120
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/321/998
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 42 No. 1 (2020); e032120
Revista Brasileira de Medicina Veterinária; v. 42 n. 1 (2020); e032120
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
_version_ 1798313107483787264

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