Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083 |
Resumo: | : This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia. |
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Semina. Ciências Agrárias (Online) |
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Babesia vogeli in dogs from Rio Branco, South-west Amazonia, BrazilBabesia vogeli em cães de Rio Branco, sudoeste da Amazônia, BrasilDiseases transmitted by ticks. Hemoparasitosis. Piroplasmosis.Doenças transmitidas por carrapatosHemoparasitosePiroplasmose.: This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia.Este é o primeiro relato de detecção molecular de Babesia vogeli em cães do estado do Acre, norte do Brasil. Este estudo teve como objetivo realizar a detecção molecular de B. vogeli em amostras de sangue de 47 cães com sinais clínicos compatíveis com hemoparasitoses no município de Rio Branco, Acre. Tratavam-se de animais atendidos em clínicas veterinárias do município, sendo realizados exames físicos, determinação do volume globular (VG), estimativa do número de plaquetas, investigação de hemoparasitos no sangue (coletado da ponta da orelha e sangue periférico). Além disso, amostras de sangue foram submetidas a extração de DNA e reação em cadeia da polimerase (PCR) para amplificação de fragmentos do gene 18S rRNA de piroplasmas. Um cão (1/47, 2,1%; IC 95%: 0,1-11,3%) apresentou resultado positivo para B. vogeli na PCR para piroplasmas e a sequência resultante mostrou 100% de identidade com os isolados de B. vogeli depositados no GenBank®. Co-infecção com Ehrlichia spp. também foi observado por exame direto (esfregaço de sangue). As alterações clínicas e hematológicas observadas no animal positivo foram anorexia, desidratação, mucosas pálidas, anemia e trombocitopenia.UEL2021-08-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4108310.5433/1679-0359.2021v42n6p3527Semina: Ciências Agrárias; Vol. 42 No. 6 (2021); 3527-3534Semina: Ciências Agrárias; v. 42 n. 6 (2021); 3527-35341679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083/30073Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFernandes, Mayara Marques PereiraRodrigues, Marcelo Renan SerrateValente, Jessica Damiana MarinhoNogueira, Marcelli PascoalFurtuoso, Rosany da Silva SantosSantos, Mirlane da SilvaVieira, Thállitha Samih Wischral JaymeVieira, Rafael Felipe da CostaSouza, Soraia Figueiredo deMalavazi, Patrícia Fernandes Nunes da Silva2022-09-30T12:32:08Zoai:ojs.pkp.sfu.ca:article/41083Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T12:32:08Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil Babesia vogeli em cães de Rio Branco, sudoeste da Amazônia, Brasil |
title |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil |
spellingShingle |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil Fernandes, Mayara Marques Pereira Diseases transmitted by ticks. Hemoparasitosis. Piroplasmosis. Doenças transmitidas por carrapatos Hemoparasitose Piroplasmose. |
title_short |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil |
title_full |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil |
title_fullStr |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil |
title_full_unstemmed |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil |
title_sort |
Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil |
author |
Fernandes, Mayara Marques Pereira |
author_facet |
Fernandes, Mayara Marques Pereira Rodrigues, Marcelo Renan Serrate Valente, Jessica Damiana Marinho Nogueira, Marcelli Pascoal Furtuoso, Rosany da Silva Santos Santos, Mirlane da Silva Vieira, Thállitha Samih Wischral Jayme Vieira, Rafael Felipe da Costa Souza, Soraia Figueiredo de Malavazi, Patrícia Fernandes Nunes da Silva |
author_role |
author |
author2 |
Rodrigues, Marcelo Renan Serrate Valente, Jessica Damiana Marinho Nogueira, Marcelli Pascoal Furtuoso, Rosany da Silva Santos Santos, Mirlane da Silva Vieira, Thállitha Samih Wischral Jayme Vieira, Rafael Felipe da Costa Souza, Soraia Figueiredo de Malavazi, Patrícia Fernandes Nunes da Silva |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Mayara Marques Pereira Rodrigues, Marcelo Renan Serrate Valente, Jessica Damiana Marinho Nogueira, Marcelli Pascoal Furtuoso, Rosany da Silva Santos Santos, Mirlane da Silva Vieira, Thállitha Samih Wischral Jayme Vieira, Rafael Felipe da Costa Souza, Soraia Figueiredo de Malavazi, Patrícia Fernandes Nunes da Silva |
dc.subject.por.fl_str_mv |
Diseases transmitted by ticks. Hemoparasitosis. Piroplasmosis. Doenças transmitidas por carrapatos Hemoparasitose Piroplasmose. |
topic |
Diseases transmitted by ticks. Hemoparasitosis. Piroplasmosis. Doenças transmitidas por carrapatos Hemoparasitose Piroplasmose. |
description |
: This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083 10.5433/1679-0359.2021v42n6p3527 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083 |
identifier_str_mv |
10.5433/1679-0359.2021v42n6p3527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083/30073 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 6 (2021); 3527-3534 Semina: Ciências Agrárias; v. 42 n. 6 (2021); 3527-3534 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306083527819264 |