Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil

Detalhes bibliográficos
Autor(a) principal: Fernandes, Mayara Marques Pereira
Data de Publicação: 2021
Outros Autores: Rodrigues, Marcelo Renan Serrate, Valente, Jessica Damiana Marinho, Nogueira, Marcelli Pascoal, Furtuoso, Rosany da Silva Santos, Santos, Mirlane da Silva, Vieira, Thállitha Samih Wischral Jayme, Vieira, Rafael Felipe da Costa, Souza, Soraia Figueiredo de, Malavazi, Patrícia Fernandes Nunes da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083
Resumo: : This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia.
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spelling Babesia vogeli in dogs from Rio Branco, South-west Amazonia, BrazilBabesia vogeli em cães de Rio Branco, sudoeste da Amazônia, BrasilDiseases transmitted by ticks. Hemoparasitosis. Piroplasmosis.Doenças transmitidas por carrapatosHemoparasitosePiroplasmose.: This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia.Este é o primeiro relato de detecção molecular de Babesia vogeli em cães do estado do Acre, norte do Brasil. Este estudo teve como objetivo realizar a detecção molecular de B. vogeli em amostras de sangue de 47 cães com sinais clínicos compatíveis com hemoparasitoses no município de Rio Branco, Acre. Tratavam-se de animais atendidos em clínicas veterinárias do município, sendo realizados exames físicos, determinação do volume globular (VG), estimativa do número de plaquetas, investigação de hemoparasitos no sangue (coletado da ponta da orelha e sangue periférico). Além disso, amostras de sangue foram submetidas a extração de DNA e reação em cadeia da polimerase (PCR) para amplificação de fragmentos do gene 18S rRNA de piroplasmas. Um cão (1/47, 2,1%; IC 95%: 0,1-11,3%) apresentou resultado positivo para B. vogeli na PCR para piroplasmas e a sequência resultante mostrou 100% de identidade com os isolados de B. vogeli depositados no GenBank®. Co-infecção com Ehrlichia spp. também foi observado por exame direto (esfregaço de sangue). As alterações clínicas e hematológicas observadas no animal positivo foram anorexia, desidratação, mucosas pálidas, anemia e trombocitopenia.UEL2021-08-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4108310.5433/1679-0359.2021v42n6p3527Semina: Ciências Agrárias; Vol. 42 No. 6 (2021); 3527-3534Semina: Ciências Agrárias; v. 42 n. 6 (2021); 3527-35341679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083/30073Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFernandes, Mayara Marques PereiraRodrigues, Marcelo Renan SerrateValente, Jessica Damiana MarinhoNogueira, Marcelli PascoalFurtuoso, Rosany da Silva SantosSantos, Mirlane da SilvaVieira, Thállitha Samih Wischral JaymeVieira, Rafael Felipe da CostaSouza, Soraia Figueiredo deMalavazi, Patrícia Fernandes Nunes da Silva2022-09-30T12:32:08Zoai:ojs.pkp.sfu.ca:article/41083Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T12:32:08Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
Babesia vogeli em cães de Rio Branco, sudoeste da Amazônia, Brasil
title Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
spellingShingle Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
Fernandes, Mayara Marques Pereira
Diseases transmitted by ticks. Hemoparasitosis. Piroplasmosis.
Doenças transmitidas por carrapatos
Hemoparasitose
Piroplasmose.
title_short Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
title_full Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
title_fullStr Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
title_full_unstemmed Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
title_sort Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil
author Fernandes, Mayara Marques Pereira
author_facet Fernandes, Mayara Marques Pereira
Rodrigues, Marcelo Renan Serrate
Valente, Jessica Damiana Marinho
Nogueira, Marcelli Pascoal
Furtuoso, Rosany da Silva Santos
Santos, Mirlane da Silva
Vieira, Thállitha Samih Wischral Jayme
Vieira, Rafael Felipe da Costa
Souza, Soraia Figueiredo de
Malavazi, Patrícia Fernandes Nunes da Silva
author_role author
author2 Rodrigues, Marcelo Renan Serrate
Valente, Jessica Damiana Marinho
Nogueira, Marcelli Pascoal
Furtuoso, Rosany da Silva Santos
Santos, Mirlane da Silva
Vieira, Thállitha Samih Wischral Jayme
Vieira, Rafael Felipe da Costa
Souza, Soraia Figueiredo de
Malavazi, Patrícia Fernandes Nunes da Silva
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Mayara Marques Pereira
Rodrigues, Marcelo Renan Serrate
Valente, Jessica Damiana Marinho
Nogueira, Marcelli Pascoal
Furtuoso, Rosany da Silva Santos
Santos, Mirlane da Silva
Vieira, Thállitha Samih Wischral Jayme
Vieira, Rafael Felipe da Costa
Souza, Soraia Figueiredo de
Malavazi, Patrícia Fernandes Nunes da Silva
dc.subject.por.fl_str_mv Diseases transmitted by ticks. Hemoparasitosis. Piroplasmosis.
Doenças transmitidas por carrapatos
Hemoparasitose
Piroplasmose.
topic Diseases transmitted by ticks. Hemoparasitosis. Piroplasmosis.
Doenças transmitidas por carrapatos
Hemoparasitose
Piroplasmose.
description : This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083
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url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083
identifier_str_mv 10.5433/1679-0359.2021v42n6p3527
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41083/30073
dc.rights.driver.fl_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 42 No. 6 (2021); 3527-3534
Semina: Ciências Agrárias; v. 42 n. 6 (2021); 3527-3534
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
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instname_str Universidade Estadual de Londrina (UEL)
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reponame_str Semina. Ciências Agrárias (Online)
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