Low density lipoprotein and egg yolk to preserve cooled donkey sêmen

• Autor(a) principal: de Melo, Maria Isabel Vaz
• Data de Publicação: 2012
• Outros Autores: Resende, Yolanda de Fátima, Rezende, Elton Pavão, Snoeck, Paola Pereira das Neves, Gomes, Daniel de Moura Lima, Carvalho, Tharley Teixeira Cardoso, Silva, Alan Vinícius Duarte, Neves, Mariana Machado, Bastos, Danilo Gonçalves, Henry, Marc Roger Jean Marie
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Brazilian Journal of Veterinary Medicine
• Texto Completo: https://rbmv.org/BJVM/article/view/748
• topic: Sêmen asinino; gema de ovo; leite; LDL; resfriamento; Donkey semen; egg yolk; milk; LDL; cooled

ABSTRACT. de Melo M.I.V., Resende Y. de F., Rezende E.P., Snoeck P.P. das N., Gomes D. de M.L., Carvalho Th.T.C., Silva A.V.D., Neves M.M., Bastos D.G. & Henry M.R.J.M.[Low density lipoprotein and egg yolk to preserve cooled donkey sêmen]. Uso de lipoproteínas de baixa densidade e de gema de ovo na preservação do sêmen asinino resfriado a 6o C. Revista Brasileira de Medicina Veterinária, 34(4):288-294, 2012. Departamento de Medicina Veterinária, Pontifícia Universidade Católica de Minas Gerais, Unidade Betim, Rua do Rosário, 1081, Bairro Angola, Betim, MG 32604- 115, Brasil, E-mail: [e-mail] The aim of this study was to evaluate the characteristics of cooled asinine sperm. Six Pêga donkeys were used. Semen was diluted 1:3 in extenders: D1 - Botusêmen ®; D2 - Botusêmen ® + 10% egg yolk; D3 - Botusêmen ® + 10% egg yolk, centrifuged at 800 X g for 15’; D4 - Botusêmen ® + 6.8% low density lipoprotein of egg yolk. The diluted semen was submitted to the cooling rate of 0.65° C / minute to achieve 5o C. It was transported and kept in a trade refrigerator at 6° C for three days. It was used the computerized system sperm analysis (CASA) to assess the characteristics of sperm every 24 hours of the cooling period. D3 (Botusêmen + egg yolk - centrifuged) was superior compared to other extenders studied the preservation of sperm characteristics, increasing storage time, mainly distinguished themselves in the percentage of motile sperm, rapidly speed, rectilinear and curvilinear velocity of displacement.