Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen

Detalhes bibliográficos
Autor(a) principal: Alonso, Carolina Natalia
Data de Publicação: 2021
Outros Autores: Castañeira, Catalina, Bragulat, Ana Flores, Losinno, Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/174301
Resumo: Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed to evaluate the effect on sperm kinetic parameters and membrane integrity in cooled donkey semen diluted with defined milk proteins extender with 1% or 2% of egg yolk and the removal of seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into four aliquots that were diluted in extender with 1% (EY1) or 2% (EY2) egg yolk. One sample from each group was centrifuged, seminal plasma was removed (CEY1, CEY2 groups, respectively), and the samples were then refrigerated at 5 °C for 24 h. Fresh and cooled semen samples were assessed for sperm motility, morphology, and plasma membrane integrity. Total motility, progressive motility, sperm kinetic parameters, or live sperm cells were not statistically different when semen was cooled with an extender supplemented with 1% or 2% of egg yolk. Seminal plasma removal does not affect total motility or sperm kinetic parameters. However, progressive motility decreased (P<0.05) when semen was extended with 2% of egg yolk and seminal plasma was removed. Membrane integrity was affected (P<0.05) in centrifuged samples. In conclusion, the obtained results suggest that there is no difference in sperm kinetics and membrane integrity when 1% or 2% of egg yolk was added to the Equiplus(R) extender. Also, the removal of seminal plasma by centrifugation did not have any beneficial effect on cooled donkey semen. Further studies are needed to relate these results with in vivo fertility tests with cooled donkey semen.
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spelling Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semenEfeito do diluente à base de gema de ovo e remoção do plasma seminal na viabilidade espermática do sêmen refrigerado de jumentoDonkeyCooled semenSemen preservationSeminal plasmaEgg yolk extenderJumentoSêmen resfriadoPreservação de sêmenPlasma seminalDiluente de gema de ovoDeveloping effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed to evaluate the effect on sperm kinetic parameters and membrane integrity in cooled donkey semen diluted with defined milk proteins extender with 1% or 2% of egg yolk and the removal of seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into four aliquots that were diluted in extender with 1% (EY1) or 2% (EY2) egg yolk. One sample from each group was centrifuged, seminal plasma was removed (CEY1, CEY2 groups, respectively), and the samples were then refrigerated at 5 °C for 24 h. Fresh and cooled semen samples were assessed for sperm motility, morphology, and plasma membrane integrity. Total motility, progressive motility, sperm kinetic parameters, or live sperm cells were not statistically different when semen was cooled with an extender supplemented with 1% or 2% of egg yolk. Seminal plasma removal does not affect total motility or sperm kinetic parameters. However, progressive motility decreased (P<0.05) when semen was extended with 2% of egg yolk and seminal plasma was removed. Membrane integrity was affected (P<0.05) in centrifuged samples. In conclusion, the obtained results suggest that there is no difference in sperm kinetics and membrane integrity when 1% or 2% of egg yolk was added to the Equiplus(R) extender. Also, the removal of seminal plasma by centrifugation did not have any beneficial effect on cooled donkey semen. Further studies are needed to relate these results with in vivo fertility tests with cooled donkey semen.O desenvolvimento de protocolos de sêmen resfriado eficazes é essencial para aumentar as taxas de prenhez e eficiência reprodutiva em jumentos. O objetivo desse estudo foi avaliar o efeito do diluente à base de proteínas do leite com 1 ou 2% de gema de ovo sobre os parâmetros cinéticos do sêmen e integridade da membrana em sêmen resfriado de jumento, com ou sem a remoção do plasma seminal. Vinte e quatro ejaculados de seis jumentos foram coletados. Cada ejaculado foi dividido em quatro alíquotas e diluído em diluente com 1% (EY1) ou 2% (EY2) de gema de ovo. Uma amostra por grupo foi centrifugada e o plasma seminal removido (grupos CEY1 e CEY2, respectivamente). Os pellets foram novamente ressuspendidos nas mesmas concentrações e diluentes. Em seguida, as quatro alíquotas foram refrigeradas a 5°C por 24 horas. Amostras de sêmen fresco e refrigerado foram avaliadas quanto à motilidade espermática e integridade da membrana plasmática. Motilidade total, motilidade progressiva, parâmetros de cinética espermática ou células espermáticas vivas não apresentaram diferença significativa quando o sêmen foi resfriado com diluente suplementado com 1% ou 2% de gema de ovo. A remoção do plasma seminal não afetou a motilidade total ou os parâmetros de cinética espermática; entretanto, a motilidade progressiva diminuiu (P<0,05) quando o sêmen foi diluído com 2% de gema de ovo e o plasma seminal removido. Nas amostras centrifugadas, a integridade da membrana foi afetada (P<0,05). Em conclusão, os resultados sugerem que não há diferença na cinética espermática e na integridade da membrana quando 1% ou 2% de gema de ovo são adicionados ao diluente Equiplus(R) e a remoção do plasma seminal por centrifugação não teve nenhum efeito benéfico no resfriamento de sêmen de jumento. Mais estudos são necessários para relacionar esses resultados com testes de fertilidade in vivo com sêmen resfriado em jumentos.  Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2021-04-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/17430110.11606/issn.1678-4456.bjvras.2021.174301Brazilian Journal of Veterinary Research and Animal Science; Vol. 58 (2021): Special Issue: Donkeys and Mules; e174301Brazilian Journal of Veterinary Research and Animal Science; Vol. 58 (2021): Special Issue: Donkeys and Mules; e174301Brazilian Journal of Veterinary Research and Animal Science; v. 58 (2021): Número especial: Asininos e Muares; e174301Brazilian Journal of Veterinary Research and Animal Science; V. 58 (2021): Special Issue: Donkeys and Mules; e1743011678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/174301/170878Copyright (c) 2021 Brazilian Journal of Veterinary Research and Animal Sciencehttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessAlonso, Carolina NataliaCastañeira, CatalinaBragulat, Ana FloresLosinno, Luis2021-07-07T11:31:34Zoai:revistas.usp.br:article/174301Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:44:07.065906Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
Efeito do diluente à base de gema de ovo e remoção do plasma seminal na viabilidade espermática do sêmen refrigerado de jumento
title Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
spellingShingle Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
Alonso, Carolina Natalia
Donkey
Cooled semen
Semen preservation
Seminal plasma
Egg yolk extender
Jumento
Sêmen resfriado
Preservação de sêmen
Plasma seminal
Diluente de gema de ovo
title_short Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
title_full Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
title_fullStr Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
title_full_unstemmed Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
title_sort Effect of egg yolk-based extender and seminal plasma removal on sperm viability of cooled donkey semen
author Alonso, Carolina Natalia
author_facet Alonso, Carolina Natalia
Castañeira, Catalina
Bragulat, Ana Flores
Losinno, Luis
author_role author
author2 Castañeira, Catalina
Bragulat, Ana Flores
Losinno, Luis
author2_role author
author
author
dc.contributor.author.fl_str_mv Alonso, Carolina Natalia
Castañeira, Catalina
Bragulat, Ana Flores
Losinno, Luis
dc.subject.por.fl_str_mv Donkey
Cooled semen
Semen preservation
Seminal plasma
Egg yolk extender
Jumento
Sêmen resfriado
Preservação de sêmen
Plasma seminal
Diluente de gema de ovo
topic Donkey
Cooled semen
Semen preservation
Seminal plasma
Egg yolk extender
Jumento
Sêmen resfriado
Preservação de sêmen
Plasma seminal
Diluente de gema de ovo
description Developing effective cooled semen protocols is essential to increase pregnancy rates and reproductive efficiency in donkeys. This study aimed to evaluate the effect on sperm kinetic parameters and membrane integrity in cooled donkey semen diluted with defined milk proteins extender with 1% or 2% of egg yolk and the removal of seminal plasma. Twenty-four ejaculates from six jackasses were collected. Each ejaculate was divided into four aliquots that were diluted in extender with 1% (EY1) or 2% (EY2) egg yolk. One sample from each group was centrifuged, seminal plasma was removed (CEY1, CEY2 groups, respectively), and the samples were then refrigerated at 5 °C for 24 h. Fresh and cooled semen samples were assessed for sperm motility, morphology, and plasma membrane integrity. Total motility, progressive motility, sperm kinetic parameters, or live sperm cells were not statistically different when semen was cooled with an extender supplemented with 1% or 2% of egg yolk. Seminal plasma removal does not affect total motility or sperm kinetic parameters. However, progressive motility decreased (P<0.05) when semen was extended with 2% of egg yolk and seminal plasma was removed. Membrane integrity was affected (P<0.05) in centrifuged samples. In conclusion, the obtained results suggest that there is no difference in sperm kinetics and membrane integrity when 1% or 2% of egg yolk was added to the Equiplus(R) extender. Also, the removal of seminal plasma by centrifugation did not have any beneficial effect on cooled donkey semen. Further studies are needed to relate these results with in vivo fertility tests with cooled donkey semen.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/174301
10.11606/issn.1678-4456.bjvras.2021.174301
url https://www.revistas.usp.br/bjvras/article/view/174301
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2021.174301
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/174301/170878
dc.rights.driver.fl_str_mv Copyright (c) 2021 Brazilian Journal of Veterinary Research and Animal Science
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Brazilian Journal of Veterinary Research and Animal Science
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 58 (2021): Special Issue: Donkeys and Mules; e174301
Brazilian Journal of Veterinary Research and Animal Science; Vol. 58 (2021): Special Issue: Donkeys and Mules; e174301
Brazilian Journal of Veterinary Research and Animal Science; v. 58 (2021): Número especial: Asininos e Muares; e174301
Brazilian Journal of Veterinary Research and Animal Science; V. 58 (2021): Special Issue: Donkeys and Mules; e174301
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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