Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/240 |
Resumo: | ABSTRACT. Bassuino D.M., Konradt G., Mari C., Cruz R.A.S., Boos G.S., Gomes D.C., Petinatti S.P. & Driemeier D. [Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus.] Achados clínicos, patológicos e imuno-histoquímicos em ovinos com raiva. Revista Brasileira de Medicina Veterinária, 38(1):86-90, 2016. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brasil. E-mail: davetpat@ufrgs.br In this article we described three cases of rabies in sheep in Rio Grande do Sul state, Brazil. Refrigerated samples of encephalon and spinal cord of two sheep, as well as one sheep sent to necropsy, with history in common of progressive neurological signs were referred to histopathological analyses. Samples from the brain and spinal cord were collected and routinely processed for hematoxylin and eosin (HE) and immunohistochemistry (IHC). To evaluate and to quantify the lesions, and also for immunohistochemistry, serial sections of the cerebral cortex, obex, thalamus, hippocampus, cerebellum and spinal cord were made. Refrigerated samples of brain were sent to direct immunofluorescence (DIF) exam for the detection of viral antigen. The histological findings were characterized by nonsuppurative meningoencephalitis and meningomyelitis with perivascular infiltrate, microgliosis, hemorrhage and congestion. Positive immunostaining was observed in the three sheep analyzed with signals ranging from discrete to accentuated. At the DIF examination, two sheep were positive and one was negative. It is concluded that rabies should be included in the differential diagnosis of diseases from the central nervous system of sheep, even if it is rarely described in this species. |
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Clinical, pathological and immunohistochemical findings of sheep infected with rabies virusAchados clínicos, patológicos e imuno-histoquímicos em ovinos com raivaRaivaovinosencéfalomedula espinhalimuno-histoquímicaRabiessheepencephalonspinal cordimmunohistochemistryABSTRACT. Bassuino D.M., Konradt G., Mari C., Cruz R.A.S., Boos G.S., Gomes D.C., Petinatti S.P. & Driemeier D. [Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus.] Achados clínicos, patológicos e imuno-histoquímicos em ovinos com raiva. Revista Brasileira de Medicina Veterinária, 38(1):86-90, 2016. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brasil. E-mail: davetpat@ufrgs.br In this article we described three cases of rabies in sheep in Rio Grande do Sul state, Brazil. Refrigerated samples of encephalon and spinal cord of two sheep, as well as one sheep sent to necropsy, with history in common of progressive neurological signs were referred to histopathological analyses. Samples from the brain and spinal cord were collected and routinely processed for hematoxylin and eosin (HE) and immunohistochemistry (IHC). To evaluate and to quantify the lesions, and also for immunohistochemistry, serial sections of the cerebral cortex, obex, thalamus, hippocampus, cerebellum and spinal cord were made. Refrigerated samples of brain were sent to direct immunofluorescence (DIF) exam for the detection of viral antigen. The histological findings were characterized by nonsuppurative meningoencephalitis and meningomyelitis with perivascular infiltrate, microgliosis, hemorrhage and congestion. Positive immunostaining was observed in the three sheep analyzed with signals ranging from discrete to accentuated. At the DIF examination, two sheep were positive and one was negative. It is concluded that rabies should be included in the differential diagnosis of diseases from the central nervous system of sheep, even if it is rarely described in this species.Descreveram-se três casos de raiva em ovinos no estado do Rio Grande do Sul. Amostras resfriadas de encéfalo e medula espinhal de dois ovinos e um ovino inteiro enviado para necropsia foram encaminhados para análise anatomopatológica, com histórico comum de apresentarem alterações neurológicas progressivas. Fragmentos dos órgãos foram colhidos e fixados em solução de formalina tamponada a 10%, processados rotineiramente para histologia e para o teste de imuno- -histoquímica (IHQ). Realizaram-se cortes seriados para avaliação e quantificação de lesões em córtex cerebral, óbex, tálamo, hipocampo, cerebelo e medula espinhal, estes também submetidos à técnica de IHQ. Amostras resfriadas de encéfalo foram enviadas para exame de imunofluorescência direta (IFD) para detecção do antígeno viral. Os achados histológicos caracterizaram-se por meningoencefalite e meningomielite não supurativa com infiltrado perivascular, microgliose, hemorragia e congestão. No exame de IHQ observou-se imunomarcação positiva nos três ovinos analisados que variou de intensidade discreta a acentuada. No exame de IFD para vírus da raiva, dois ovinos foram positivos e um resultou negativo. Conclui-se que a raiva deve ser incluída no diagnóstico diferencial das doenças do sistema nervoso central de ovinos, mesmo que seja raramente descrita nesta espécie.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2016-03-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/24010.2430/00000000000000Brazilian Journal of Veterinary Medicine; Vol. 38 No. 1 (2016); 86-90Revista Brasileira de Medicina Veterinária; v. 38 n. 1 (2016); 86-902527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/240/161Bassuino, Daniele MariathKonradt, GuilhermeMari, Cristineda Cruz, Raquel Aparecida SalesBoos, Gisele SilvaGomes, Danilo CarlotoPavarini, Saulo PetinattiDriemeier, Davidinfo:eu-repo/semantics/openAccess2020-12-23T17:26:24Zoai:ojs.rbmv.org:article/240Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:26:24Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus Achados clínicos, patológicos e imuno-histoquímicos em ovinos com raiva |
title |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus |
spellingShingle |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus Bassuino, Daniele Mariath Raiva ovinos encéfalo medula espinhal imuno-histoquímica Rabies sheep encephalon spinal cord immunohistochemistry |
title_short |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus |
title_full |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus |
title_fullStr |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus |
title_full_unstemmed |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus |
title_sort |
Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus |
author |
Bassuino, Daniele Mariath |
author_facet |
Bassuino, Daniele Mariath Konradt, Guilherme Mari, Cristine da Cruz, Raquel Aparecida Sales Boos, Gisele Silva Gomes, Danilo Carloto Pavarini, Saulo Petinatti Driemeier, David |
author_role |
author |
author2 |
Konradt, Guilherme Mari, Cristine da Cruz, Raquel Aparecida Sales Boos, Gisele Silva Gomes, Danilo Carloto Pavarini, Saulo Petinatti Driemeier, David |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Bassuino, Daniele Mariath Konradt, Guilherme Mari, Cristine da Cruz, Raquel Aparecida Sales Boos, Gisele Silva Gomes, Danilo Carloto Pavarini, Saulo Petinatti Driemeier, David |
dc.subject.por.fl_str_mv |
Raiva ovinos encéfalo medula espinhal imuno-histoquímica Rabies sheep encephalon spinal cord immunohistochemistry |
topic |
Raiva ovinos encéfalo medula espinhal imuno-histoquímica Rabies sheep encephalon spinal cord immunohistochemistry |
description |
ABSTRACT. Bassuino D.M., Konradt G., Mari C., Cruz R.A.S., Boos G.S., Gomes D.C., Petinatti S.P. & Driemeier D. [Clinical, pathological and immunohistochemical findings of sheep infected with rabies virus.] Achados clínicos, patológicos e imuno-histoquímicos em ovinos com raiva. Revista Brasileira de Medicina Veterinária, 38(1):86-90, 2016. Programa de Pós-Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brasil. E-mail: davetpat@ufrgs.br In this article we described three cases of rabies in sheep in Rio Grande do Sul state, Brazil. Refrigerated samples of encephalon and spinal cord of two sheep, as well as one sheep sent to necropsy, with history in common of progressive neurological signs were referred to histopathological analyses. Samples from the brain and spinal cord were collected and routinely processed for hematoxylin and eosin (HE) and immunohistochemistry (IHC). To evaluate and to quantify the lesions, and also for immunohistochemistry, serial sections of the cerebral cortex, obex, thalamus, hippocampus, cerebellum and spinal cord were made. Refrigerated samples of brain were sent to direct immunofluorescence (DIF) exam for the detection of viral antigen. The histological findings were characterized by nonsuppurative meningoencephalitis and meningomyelitis with perivascular infiltrate, microgliosis, hemorrhage and congestion. Positive immunostaining was observed in the three sheep analyzed with signals ranging from discrete to accentuated. At the DIF examination, two sheep were positive and one was negative. It is concluded that rabies should be included in the differential diagnosis of diseases from the central nervous system of sheep, even if it is rarely described in this species. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/240 10.2430/00000000000000 |
url |
https://rbmv.org/BJVM/article/view/240 |
identifier_str_mv |
10.2430/00000000000000 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/240/161 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 38 No. 1 (2016); 86-90 Revista Brasileira de Medicina Veterinária; v. 38 n. 1 (2016); 86-90 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313107000393728 |