In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/1014 |
Resumo: | The present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflammation, being most often infected cells neurons. The neuronal degeneration involved 100% of cases, in addition to a diffuse lymphocytic Infiltration and gliosis, characterized by vasculitis and perivasculitis. The presence of Negri bodies was in most cases in discreet, and the fragments with higher concentrations of antigen by both techniques employed were the cerebellum and the brain stem. Immunohistochemistry test (IHC) demonstrated greater sensitivity when applied to samples of bovines. Our results showed that in 37.5% of the total number of fragments analyzed, viral inclusions were not observed, however, there was an inflammatory process. In relation to the species, the fragments from bovine’s animals showed a slight increase when examined under this feature. These findings highlight the importance of submitting samples from suspected animals for laboratory diagnostic, even when there are no apparent abnormal histological findings. |
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Brazilian Journal of Veterinary Medicine |
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In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovinesCaracterização histopatológica e imuno-histoquímica In situ do vírus da raiva em cérebros de equinos e bovinos naturalmente infectadosPalavras-chave: raiva, equinos, bovinos, Imuno-histoquímica, IFD, alterações histopatológicasKeywords: rabies, equines, bovines, Immunohistochemistry, FAT, histopathological changesThe present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflammation, being most often infected cells neurons. The neuronal degeneration involved 100% of cases, in addition to a diffuse lymphocytic Infiltration and gliosis, characterized by vasculitis and perivasculitis. The presence of Negri bodies was in most cases in discreet, and the fragments with higher concentrations of antigen by both techniques employed were the cerebellum and the brain stem. Immunohistochemistry test (IHC) demonstrated greater sensitivity when applied to samples of bovines. Our results showed that in 37.5% of the total number of fragments analyzed, viral inclusions were not observed, however, there was an inflammatory process. In relation to the species, the fragments from bovine’s animals showed a slight increase when examined under this feature. These findings highlight the importance of submitting samples from suspected animals for laboratory diagnostic, even when there are no apparent abnormal histological findings. O presente estudo buscou caracterizar os fenômenos envolvidos na histopatologia da raiva e avaliar a presença e quantidade de antígeno viral in situ nas diferentes regiões cerebrais de equinos e bovinos naturalmente infectados. O exame histopatológico demonstrou várias mudanças devido à inflamação, sendo mais frequentemente infectadas as células neuronais. A degeneração neuronal foi observada em 100% dos casos, além de uma infiltração linfocitária difusa e gliose, caracterizada por vasculite e perivasculite. A presença de corpúsculos de Negri foi observada na maioria dos casos de maneira discreta, e os fragmentos com maior concentração de antígeno, por ambos os testes empregadas foram o cerebelo e o tronco encefálico. O teste de Imuno-histoquímica (IHC) demonstrou maior sensibilidade quando aplicada em amostras de bovinos. Nossos resultados demostraram que em 37,5% do número total de fragmentos analisados, inclusões virais não foram observadas, no entanto, havia um processo inflamatório. Em relação à espécie, os fragmentos de bovinos demonstraram um ligeiro aumento quando examinado sob este aspecto. Esses achados destacam a importância de submeter amostras de animais suspeitos para diagnóstico laboratorial, mesmo quando não houver nenhum achado histopatológico anormal. Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2019-09-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/101410.29374/2527-2179.bjvm101419Brazilian Journal of Veterinary Medicine; Vol. 41 No. 1 (2019); e101419Revista Brasileira de Medicina Veterinária; v. 41 n. 1 (2019); e1014192527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVenghttps://rbmv.org/BJVM/article/view/1014/958Copyright (c) 2019 Samira Maria Achkar, Elaine Raniero Fernandes, Fernanda Guedes, Enio Mori, Carla Isabel Macedo, Maria Irma e Seixas Duarteinfo:eu-repo/semantics/openAccessAchkar, Samira MariaFernandes, Elaine RanieroGuedes, FernandaMori, EnioMacedo, Carla IsabelDuarte, Maria Irma e Seixas2020-12-23T17:24:50Zoai:ojs.rbmv.org:article/1014Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:24:50Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines Caracterização histopatológica e imuno-histoquímica In situ do vírus da raiva em cérebros de equinos e bovinos naturalmente infectados |
title |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
spellingShingle |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines Achkar, Samira Maria Palavras-chave: raiva, equinos, bovinos, Imuno-histoquímica, IFD, alterações histopatológicas Keywords: rabies, equines, bovines, Immunohistochemistry, FAT, histopathological changes |
title_short |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
title_full |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
title_fullStr |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
title_full_unstemmed |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
title_sort |
In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines |
author |
Achkar, Samira Maria |
author_facet |
Achkar, Samira Maria Fernandes, Elaine Raniero Guedes, Fernanda Mori, Enio Macedo, Carla Isabel Duarte, Maria Irma e Seixas |
author_role |
author |
author2 |
Fernandes, Elaine Raniero Guedes, Fernanda Mori, Enio Macedo, Carla Isabel Duarte, Maria Irma e Seixas |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Achkar, Samira Maria Fernandes, Elaine Raniero Guedes, Fernanda Mori, Enio Macedo, Carla Isabel Duarte, Maria Irma e Seixas |
dc.subject.por.fl_str_mv |
Palavras-chave: raiva, equinos, bovinos, Imuno-histoquímica, IFD, alterações histopatológicas Keywords: rabies, equines, bovines, Immunohistochemistry, FAT, histopathological changes |
topic |
Palavras-chave: raiva, equinos, bovinos, Imuno-histoquímica, IFD, alterações histopatológicas Keywords: rabies, equines, bovines, Immunohistochemistry, FAT, histopathological changes |
description |
The present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflammation, being most often infected cells neurons. The neuronal degeneration involved 100% of cases, in addition to a diffuse lymphocytic Infiltration and gliosis, characterized by vasculitis and perivasculitis. The presence of Negri bodies was in most cases in discreet, and the fragments with higher concentrations of antigen by both techniques employed were the cerebellum and the brain stem. Immunohistochemistry test (IHC) demonstrated greater sensitivity when applied to samples of bovines. Our results showed that in 37.5% of the total number of fragments analyzed, viral inclusions were not observed, however, there was an inflammatory process. In relation to the species, the fragments from bovine’s animals showed a slight increase when examined under this feature. These findings highlight the importance of submitting samples from suspected animals for laboratory diagnostic, even when there are no apparent abnormal histological findings. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/1014 10.29374/2527-2179.bjvm101419 |
url |
https://rbmv.org/BJVM/article/view/1014 |
identifier_str_mv |
10.29374/2527-2179.bjvm101419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/1014/958 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 41 No. 1 (2019); e101419 Revista Brasileira de Medicina Veterinária; v. 41 n. 1 (2019); e101419 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313110567649280 |