Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Jornal Brasileiro de Nefrologia |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002018000100059 |
Resumo: | Abstract Introduction: BK virus (BKV) infection in renal transplant patients may cause kidney allograft dysfunction and graft loss. Accurate determination of BKV viral load is critical to prevent BKV-associated nephropathy (BKVAN) but the cut-off that best predicts BKVAN remains controversial. Objective: To evaluate the performance of a commercial and an in-house qPCR test for quantitative detection of BK virus in kidney transplant recipients. Methods: This was a prospective study with kidney transplant recipients from two large university hospitals in Brazil. Patients were screened for BKV infection every 3 months in the first year post-transplant with a commercial and an in-house real time polymerase chain reaction (qPCR) test. BKVAN was confirmed based on histopathology. The area under the curve for plasma qPCR was determined from receiver operating characteristic analysis. Results: A total of 200 patients were enrolled. Fifty-eight percent were male, 19.5% had diabetes mellitus, and 82% had the kidney transplanted from a deceased donor. BKV viremia was detected in 32.5% and BKVAN was diagnosed in 8 patients (4%). BKVAN was associated with viremia of 4.1 log copies/mL, using a commercial kit. The cut-off for the in-house assay was 6.1 log copies/mL. The linearity between the commercial kit and the in-house assay was R2=0.83. Conclusion: Our study shows that marked variability occurs in BKV viral load when different qPCR methodologies are used. The in-house qPCR assay proved clinically useful, a cheaper option in comparison to commercial qPCR kits. There is an urgent need to make BKV standards available to the international community. |
id |
SBN-1_3f7b22ff2419ec340138e695e27780bd |
---|---|
oai_identifier_str |
oai:scielo:S0101-28002018000100059 |
network_acronym_str |
SBN-1 |
network_name_str |
Jornal Brasileiro de Nefrologia |
repository_id_str |
|
spelling |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation studyKidney TransplantationViremiaPolymerase Chain ReactionPolyomavirusAbstract Introduction: BK virus (BKV) infection in renal transplant patients may cause kidney allograft dysfunction and graft loss. Accurate determination of BKV viral load is critical to prevent BKV-associated nephropathy (BKVAN) but the cut-off that best predicts BKVAN remains controversial. Objective: To evaluate the performance of a commercial and an in-house qPCR test for quantitative detection of BK virus in kidney transplant recipients. Methods: This was a prospective study with kidney transplant recipients from two large university hospitals in Brazil. Patients were screened for BKV infection every 3 months in the first year post-transplant with a commercial and an in-house real time polymerase chain reaction (qPCR) test. BKVAN was confirmed based on histopathology. The area under the curve for plasma qPCR was determined from receiver operating characteristic analysis. Results: A total of 200 patients were enrolled. Fifty-eight percent were male, 19.5% had diabetes mellitus, and 82% had the kidney transplanted from a deceased donor. BKV viremia was detected in 32.5% and BKVAN was diagnosed in 8 patients (4%). BKVAN was associated with viremia of 4.1 log copies/mL, using a commercial kit. The cut-off for the in-house assay was 6.1 log copies/mL. The linearity between the commercial kit and the in-house assay was R2=0.83. Conclusion: Our study shows that marked variability occurs in BKV viral load when different qPCR methodologies are used. The in-house qPCR assay proved clinically useful, a cheaper option in comparison to commercial qPCR kits. There is an urgent need to make BKV standards available to the international community.Sociedade Brasileira de Nefrologia2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002018000100059Brazilian Journal of Nephrology v.40 n.1 2018reponame:Jornal Brasileiro de Nefrologiainstname:Sociedade Brasileira de Nefrologia (SBN)instacron:SBN10.1590/1678-4685-jbn-3776info:eu-repo/semantics/openAccessPinto,Gabriel GodinhoPoloni,José Antonio TesserPaskulin,Diego D'AvilaSpuldaro,FabioParis,Fernanda deBarth,Afonso LuísManfro,Roberto CerattiKeitel,ElizetePasqualotto,Alessandro C.eng2018-05-11T00:00:00Zoai:scielo:S0101-28002018000100059Revistahttp://www.bjn.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||jbn@sbn.org.br2175-82390101-2800opendoar:2018-05-11T00:00Jornal Brasileiro de Nefrologia - Sociedade Brasileira de Nefrologia (SBN)false |
dc.title.none.fl_str_mv |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
title |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
spellingShingle |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study Pinto,Gabriel Godinho Kidney Transplantation Viremia Polymerase Chain Reaction Polyomavirus |
title_short |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
title_full |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
title_fullStr |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
title_full_unstemmed |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
title_sort |
Quantitative detection of BK virus in kidney transplant recipients: a prospective validation study |
author |
Pinto,Gabriel Godinho |
author_facet |
Pinto,Gabriel Godinho Poloni,José Antonio Tesser Paskulin,Diego D'Avila Spuldaro,Fabio Paris,Fernanda de Barth,Afonso Luís Manfro,Roberto Ceratti Keitel,Elizete Pasqualotto,Alessandro C. |
author_role |
author |
author2 |
Poloni,José Antonio Tesser Paskulin,Diego D'Avila Spuldaro,Fabio Paris,Fernanda de Barth,Afonso Luís Manfro,Roberto Ceratti Keitel,Elizete Pasqualotto,Alessandro C. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pinto,Gabriel Godinho Poloni,José Antonio Tesser Paskulin,Diego D'Avila Spuldaro,Fabio Paris,Fernanda de Barth,Afonso Luís Manfro,Roberto Ceratti Keitel,Elizete Pasqualotto,Alessandro C. |
dc.subject.por.fl_str_mv |
Kidney Transplantation Viremia Polymerase Chain Reaction Polyomavirus |
topic |
Kidney Transplantation Viremia Polymerase Chain Reaction Polyomavirus |
description |
Abstract Introduction: BK virus (BKV) infection in renal transplant patients may cause kidney allograft dysfunction and graft loss. Accurate determination of BKV viral load is critical to prevent BKV-associated nephropathy (BKVAN) but the cut-off that best predicts BKVAN remains controversial. Objective: To evaluate the performance of a commercial and an in-house qPCR test for quantitative detection of BK virus in kidney transplant recipients. Methods: This was a prospective study with kidney transplant recipients from two large university hospitals in Brazil. Patients were screened for BKV infection every 3 months in the first year post-transplant with a commercial and an in-house real time polymerase chain reaction (qPCR) test. BKVAN was confirmed based on histopathology. The area under the curve for plasma qPCR was determined from receiver operating characteristic analysis. Results: A total of 200 patients were enrolled. Fifty-eight percent were male, 19.5% had diabetes mellitus, and 82% had the kidney transplanted from a deceased donor. BKV viremia was detected in 32.5% and BKVAN was diagnosed in 8 patients (4%). BKVAN was associated with viremia of 4.1 log copies/mL, using a commercial kit. The cut-off for the in-house assay was 6.1 log copies/mL. The linearity between the commercial kit and the in-house assay was R2=0.83. Conclusion: Our study shows that marked variability occurs in BKV viral load when different qPCR methodologies are used. The in-house qPCR assay proved clinically useful, a cheaper option in comparison to commercial qPCR kits. There is an urgent need to make BKV standards available to the international community. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002018000100059 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002018000100059 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-jbn-3776 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Nefrologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Nefrologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Nephrology v.40 n.1 2018 reponame:Jornal Brasileiro de Nefrologia instname:Sociedade Brasileira de Nefrologia (SBN) instacron:SBN |
instname_str |
Sociedade Brasileira de Nefrologia (SBN) |
instacron_str |
SBN |
institution |
SBN |
reponame_str |
Jornal Brasileiro de Nefrologia |
collection |
Jornal Brasileiro de Nefrologia |
repository.name.fl_str_mv |
Jornal Brasileiro de Nefrologia - Sociedade Brasileira de Nefrologia (SBN) |
repository.mail.fl_str_mv |
||jbn@sbn.org.br |
_version_ |
1752122064638050304 |