BK virus salivary shedding and viremia in renal transplant recipients
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/158642 |
Resumo: | Objectives: This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia. Material and Methods: We have conducted a crosssectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman’s correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients. Results: The mean age of the study group was 51.11±12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80±6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86±5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman’s correlation coefficient=0.193). Conclusion: The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication. |
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Journal of applied oral science (Online) |
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BK virus salivary shedding and viremia in renal transplant recipientsTransplantationBK virusSalivaImmunocompromised hostPolymerase chain reactionObjectives: This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia. Material and Methods: We have conducted a crosssectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman’s correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients. Results: The mean age of the study group was 51.11±12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80±6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86±5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman’s correlation coefficient=0.193). Conclusion: The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication.Universidade de São Paulo. Faculdade de Odontologia de Bauru2019-06-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/15864210.1590/1678-7757-2018-0435Journal of Applied Oral Science; Vol. 27 (2019); e20180435Journal of Applied Oral Science; Vol. 27 (2019); e20180435Journal of Applied Oral Science; v. 27 (2019); e201804351678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/158642/153652Copyright (c) 2019 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSarmento, Dmitry José de SantanaPalmieri, MichelleGalvão, Gustavo SouzaTozetto-Mendoza, Tânia ReginaCanto, Cynthia Motta doPierrotti, Ligia CameraDavid-Neto, EliasAgena, FabianaGallottini, MarinaPannuti, Claudio SergioFink, Maria Cristina DominguesBraz-Silva, Paulo Henrique2019-06-06T16:06:27Zoai:revistas.usp.br:article/158642Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2019-06-06T16:06:27Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
BK virus salivary shedding and viremia in renal transplant recipients |
title |
BK virus salivary shedding and viremia in renal transplant recipients |
spellingShingle |
BK virus salivary shedding and viremia in renal transplant recipients Sarmento, Dmitry José de Santana Transplantation BK virus Saliva Immunocompromised host Polymerase chain reaction |
title_short |
BK virus salivary shedding and viremia in renal transplant recipients |
title_full |
BK virus salivary shedding and viremia in renal transplant recipients |
title_fullStr |
BK virus salivary shedding and viremia in renal transplant recipients |
title_full_unstemmed |
BK virus salivary shedding and viremia in renal transplant recipients |
title_sort |
BK virus salivary shedding and viremia in renal transplant recipients |
author |
Sarmento, Dmitry José de Santana |
author_facet |
Sarmento, Dmitry José de Santana Palmieri, Michelle Galvão, Gustavo Souza Tozetto-Mendoza, Tânia Regina Canto, Cynthia Motta do Pierrotti, Ligia Camera David-Neto, Elias Agena, Fabiana Gallottini, Marina Pannuti, Claudio Sergio Fink, Maria Cristina Domingues Braz-Silva, Paulo Henrique |
author_role |
author |
author2 |
Palmieri, Michelle Galvão, Gustavo Souza Tozetto-Mendoza, Tânia Regina Canto, Cynthia Motta do Pierrotti, Ligia Camera David-Neto, Elias Agena, Fabiana Gallottini, Marina Pannuti, Claudio Sergio Fink, Maria Cristina Domingues Braz-Silva, Paulo Henrique |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sarmento, Dmitry José de Santana Palmieri, Michelle Galvão, Gustavo Souza Tozetto-Mendoza, Tânia Regina Canto, Cynthia Motta do Pierrotti, Ligia Camera David-Neto, Elias Agena, Fabiana Gallottini, Marina Pannuti, Claudio Sergio Fink, Maria Cristina Domingues Braz-Silva, Paulo Henrique |
dc.subject.por.fl_str_mv |
Transplantation BK virus Saliva Immunocompromised host Polymerase chain reaction |
topic |
Transplantation BK virus Saliva Immunocompromised host Polymerase chain reaction |
description |
Objectives: This study aimed to verify the presence of polyomavirus BK (BKPyV) in the saliva of kidney transplant recipients and to correlate it with blood viremia. Material and Methods: We have conducted a crosssectional study with a sample involving 126 renal transplant recipients. 126 samples of saliva and 52 samples of blood were collected from these patients. Detection and quantification of BKPyV were performed using a real-time PCR. To compare the presence of BKPyV in blood and saliva, the binomial proportion test was used. To verify associations between salivary shedding BKPyV and post-transplant periods (in months), the Mann-Whitney test was used. Spearman’s correlation was used to correlate the viral load in the saliva with blood of kidney transplant recipients. Results: The mean age of the study group was 51.11±12.45 years old, and 69 participants (54.8%) were female, with a mean post-transplantation time of 4.80±6.04 months. BKPyV was quantified in several samples of saliva and blood, with medians of 1,108 cp/mL and 1,255 cp/mL, respectively. Only 16/52 (30.8%) participants presented BKPyV in blood, and 59/126 (46.8%) excreted the virus in saliva (p=0.004). BKPyV shedding was found in patients at a shorter post-transplantation period (3.86±5.25, p=0.100). A weak correlation was observed between viral quantification in saliva and blood (Spearman’s correlation coefficient=0.193). Conclusion: The results of this study suggested that, although saliva excretes more BKPyV than blood, there is no reliable correlation between salivary shedding and blood viremia, showing two independent compartments of viral replication. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/158642 10.1590/1678-7757-2018-0435 |
url |
https://www.revistas.usp.br/jaos/article/view/158642 |
identifier_str_mv |
10.1590/1678-7757-2018-0435 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/158642/153652 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 27 (2019); e20180435 Journal of Applied Oral Science; Vol. 27 (2019); e20180435 Journal of Applied Oral Science; v. 27 (2019); e20180435 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681152688128 |